Fast liquid chromatography for the determination of drugs in plasma and combination with liquid—solid extraction in a fully automated system

Rouan, Marie-Claude; Duigou, F. Le; Campestrini, Joelle; Lecaillon, J.B.; Godbillon, J.

doi:10.1016/0378-4347(92)80474-5

Cited by 8 publications

(5 citation statements)

References 9 publications

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“…Concentrations of TCBZ and metabolites in plasma were measured by h.p.l.c. [7]. The limit of quantification was 0.06 μmol l −1 (0.02 μg ml −1 ) for TCBZ, 0.13 μmol l −1 (0.05 μg ml −1 ) for TCBZ‐SO and 0.05 μmol l −1 (0.02 μg ml −1 ) for TCBZ‐SO2.…”

Section: Methodsmentioning

confidence: 99%

Effect of food on the bioavailability of triclabendazole in patients with fascioliasis

Lecaillon

Godbillon

Campestrini

et al. 1998

Brit J Clinical Pharma

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Aims Preliminary results indicate higher absorption of triclabendazole (TCBZ) administered postprandially. Therefore, the influence of food on the pharmacokinetics of TCBZ and its active sulphoxide (TCBZ-SO) and sulphone (TCBZ-SO2) metabolites was investigated. Methods Two single doses (10 mg kg −1 ) of TCBZ were administered to 20 patients with fascioliasis. Ten patients were first given the drug after a high energy breakfast and then, 48 h later, after an overnight fast. The other 10 patients first received the drug in fasting state and then, 48 h later, after breakfast. A low energy breakfast was served 2 h after drug administration for fasting state.Results Compared with the fasting state, an increased AUC and C max after food intake (significant, P<0 . Tolerability to the treatment among the patients was good. Conclusions The administration of triclabendazole with food is recommended for improved systemic availability in patients with fascioliasis or paragonimiasis.

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Section: Methodsmentioning

confidence: 99%

Effect of food on the bioavailability of triclabendazole in patients with fascioliasis

Lecaillon

Godbillon

Campestrini

et al. 1998

Brit J Clinical Pharma

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“…Rouan et al [154] described an automated C 18 clean-up procedure for isolation of TCB residues on an ASPEC system. They demonstrated, with a limited number of samples, that recovery values greater than 90% could be obtained under optimal conditions.…”

Section: Plasma Serum or Other Biological Fluidsmentioning

confidence: 99%

“…Rouan et al separated TCB, TCB-SO, TCB-SO 2 and an internal standard on a short C 18 column (33 mm × 4.6 mm) in less than 10 min [154]. Porter and Johnston [232] evaluated narrow bore columns (2.1 mm) for determination of TBZ, MBZ, ABZ and FBZ residues.…”

Section: Liquid Chromatography Based Separationsmentioning

confidence: 99%

Review of methodology for the determination of benzimidazole residues in biological matrices

Danaher

Ruyck

Crooks

et al. 2007

Journal of Chromatography B

186

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“…The robotic method was adopted from a manual HPLC method (Rouan et al, 1991). Utilization of a laboratory robotic system eliminates the tedium of the sample preparation procedure, improves safety by ensuring minimal exposure to biolo- gical samples, and frees the analyst to perform other duties, by leaving only the replenishment of disposables (tubes, caps and pipette tips) and filling of reagent reservoirs.…”

Section: Discussionmentioning

confidence: 99%

“…The manual method involving liquid-liquid extraction previously described for the assay of CGP 33101 in plasma (Rouan et al, 1991) has been reproduced by the robotic system with minor modifications. The entire system provides unattended sample preparation and analysis, as well as automated retrieval and computation of the chromatographic results. )…”

Section: Introductionmentioning

confidence: 99%

An automated method for the determination of a new potential antiepileptic agent (CGP 33101) in human plasma using high performance liquid chromatography

Brunner¹,

Powell²

1992

Biomedical Chromatography

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An automated analytical method utilizing laboratory robotics has been developed and validated for quantifying concentrations of a new antiepileptic drug candidate (CGP 33101) in human plasma. The robotic system aliquots the biological sample, adds the internal standard (CGP 23901) and pH 12 buffer, extracts the compounds from the basified matrix into an organic phase (methyl-t-butyl ether:dichloromethane, 2:1) and concentrates the extracts for reversed-phase, high performance liquid chromatographic (HPLC) analysis. The robotic system is directly interfaced with the HPLC system. Separation is achieved on a Hypersil 3 microns C18 column (4.6 x 50 mm) with ultraviolet detection of the analytes at 230 nm. Specificity was demonstrated by the lack of interfering peaks at the retention times for both the drug and internal standard. Recovery and reproducibility assessments indicated good accuracy (overall mean relative recovery of 102.7%) and precision (coefficient of variation of 4.4 to 7.7%) for CGP 33101 over the concentration range of 50-4000 ng/mL. The limit of quantification (LOQ) is 50 ng/mL. The method has been successfully applied to a clinical study in which normal volunteers received single oral doses of 400-1200 mg of this new drug candidate.

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Fast liquid chromatography for the determination of drugs in plasma and combination with liquid—solid extraction in a fully automated system

Cited by 8 publications

References 9 publications

Effect of food on the bioavailability of triclabendazole in patients with fascioliasis

Effect of food on the bioavailability of triclabendazole in patients with fascioliasis

Review of methodology for the determination of benzimidazole residues in biological matrices

An automated method for the determination of a new potential antiepileptic agent (CGP 33101) in human plasma using high performance liquid chromatography

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