Fast measurement of serum amyloid A in different specimens from swine by using a new one-step time-resolved fluorescent immunoassay

Inflammation is a facilitating process for multiple cancer types. It is believed to affect cancer development and progression through several etiologic pathways including increased levels of DNA adduct formation, increased angiogenesis and altered anti-apoptotic signaling. This review highlights the application of inflammatory biomarkers in epidemiologic studies and discusses the various cellular mediators of inflammation characterizing the innate immune system response to infection and chronic insult from environmental factors. Included is a review of six classes of inflammation-related biomarkers: cytokines/chemokines, immune-related effectors, acute phase proteins, reactive oxygen and nitrogen species, prostaglandins and cyclooxygenase-related factors, and mediators such as transcription factors and growth factors. For each of these biomarkers we provide a brief overview of the etiologic role in the inflammation response and how they have been related to cancer etiology and progression within the literature. We provide a discussion of the common techniques available for quantification of each marker including strengths, weaknesses and potential pitfalls. Subsequently, we highlight a few under-studied measures to characterize the inflammatory response and their potential utility in epidemiologic studies of cancer. Finally, we suggest integrative methods for future studies to apply multi-faceted approaches to examine the relationship between inflammatory markers and their roles in cancer development.

show abstract

“…Levels can also be detected in saliva using different techniques including fluorescent immunoassays (157). …”

Section: Acute Phase Proteinsmentioning

confidence: 99%

A Review of the Application of Inflammatory Biomarkers in Epidemiologic Cancer Research

Brenner

Scherer

Muir

et al. 2014

Cancer Epidemiology, Biomarkers &Amp; Prevention

146

123

View full text Add to dashboard Cite

show abstract

“…In fact, the use of saliva as an analytical tool in porcine research has increased exponentially over the last years according to the PubMed database. Several studies have indicated that salivary acute phase protein (APP) concentrations can be utilized as early indicators of the health status of pigs (2, 3). This statement is of great value taking into account that some diseased pigs did not develop leucocytosis in spite of having clinical signs and pathological lesions but experienced an evident increase in APPs (4, 5).…”

Section: Introductionmentioning

confidence: 99%

Porcine Breed, Sex, and Production Stage Influence the Levels of Health Status Biomarkers in Saliva Samples

Sánchez¹,

García

Ruiz

et al. 2019

Front. Vet. Sci.

View full text Add to dashboard Cite

In this study, the influence of several factors such as breed, sex, and production stage over the normal range values of salivary biomarkers of health status was evaluated in pigs. A total of 409 pigs of 2 different breeds (conventional Large White × Duroc and Iberian pigs) were included in the study. Animals were divided into different groups according to their sex (male or female) and the stage of the production cycle they were in (post-weaning, nursery, fattening, and finishing). The levels of an inflammatory marker, adenosine deaminase (ADA), and two acute phase proteins, C-reactive protein (CRP) and haptoglobin (Hp) were measured in saliva samples. Moreover, the total antioxidant capacity level (TAC) was quantified for the first time in porcine saliva; therefore, an analytical validation and stability analysis during storage at −80°C were also performed. Differences according to breed were observed for all the markers studied; thus, the influence of age and sex on the normal range values were studied separately for conventional and Iberian pigs. In Large White × Duroc pigs the overall median values of ADA, CRP, Hp and TAC were 282 U/L, 10.49 ng/mL, 0.88 μg/mL, and 21.73 μM Trolox equivalents, respectively. However, higher values of inflammatory marker and acute phase proteins were observed in males at the initial stages of the production cycle, while females presented higher values when they had reached sexual maturity. In Iberian pigs the overall median values observed were 585 U/L, 4.81 ng/mL, 0.63 μg/mL, and 21.21 μM Trolox equivalents for ADA, CRP, Hp, and TAC respectively with slight differences in the influence of the studied factors. Sex differences were not observed in the levels of acute phase proteins in Iberian pigs, probably due to the castration of males during the first days of life; however, ADA levels were found to be higher in male pigs at the end of the production cycle. It could be concluded that breed, sex, and production stage influence the range values of salivary markers of health status in pigs and should be taken into account to further establish reference intervals.

show abstract

“…While the concentration of SAA in the serum is typically 1.0–10 μg/mL in healthy individuals, it has been observed to increase up to 1 mg/mL during illness . Previous studies in the literature have determined the measurements of SAA in swine samples of serum and saliva using a time-resolved fluorescent immunoassay (TR-FIA) …”

Section: Resultsmentioning

confidence: 99%

Electrospun Poly-ε-caprolactone/Poly-l-lysine (PCL/PLL) Nanofibers as an Emergent Material for the Preparation of Electrochemical Immunosensor to Detect Serum Amyloid A

Ozturk Kirbay,

Odaci

2024

ACS Appl. Polym. Mater.

View full text Add to dashboard Cite

Serum amyloid (SAA), one of the acute-phase reactants, plays a significant role in acute and chronic inflammatory diseases. It is widely used in the clinical field as a biomarker of inflammation. Developing rapid and sensitive measurement systems is crucial to precisely detect the presence of SAA, even at very low levels. Therefore, in this study, we developed an electrochemical immunosensor using electrospun nanofibers (ESNFs) based on poly-ε-caprolactone (PCL) and poly-L-Lysine (PLL) as an immobilization matrix. ESNFs covered the surfaces of screenprinted carbon electrodes (SPCEs) for enhanced performance. The PCL/PLL ESNFs were characterized using water contact angle measurements, scanning electron microscopy (SEM), Brunauer−Emmett−Teller (BET) method, X-ray photoelectron spectroscopy (XPS), and Fourier transform infrared (FTIR) spectroscopy techniques. Cyclic voltammetry (CV), differential pulse voltammetry (DPV), and impedance spectroscopy (EIS) were employed to characterize the electrochemical behavior of the developed electrode surface. In conjunction with the analytical characterizations of the proposed immunosensor, linear range, limit of detection (LOD), interfering effect of some potential chemicals, and real sample applications were carried out. The sensor exhibited a working range and the linear range of 0.1−50 and 0.1−10 ng/mL with a calculated LOD of 0.061 ng/mL for SAA, respectively. Additionally, the sensor demonstrated advanced specificity, showing no response to the interfering species such as urea, glucose, insulin, and Creactive protein. Validation of the sensor yielded excellent recovery for the artificial serum (100.04%), human real serum (103.32%), and artificial saliva (99.5%) samples containing 1 ng/mL SAA. Therefore, we believe that the sensor, with its high specificity, and sensitivity, can effectively contribute to the detection of SAA at low levels in biological fluids during clinical tests.

show abstract

Fast measurement of serum amyloid A in different specimens from swine by using a new one-step time-resolved fluorescent immunoassay

Cited by 12 publications

References 16 publications

A Review of the Application of Inflammatory Biomarkers in Epidemiologic Cancer Research

A Review of the Application of Inflammatory Biomarkers in Epidemiologic Cancer Research

Porcine Breed, Sex, and Production Stage Influence the Levels of Health Status Biomarkers in Saliva Samples

Electrospun Poly-ε-caprolactone/Poly-l-lysine (PCL/PLL) Nanofibers as an Emergent Material for the Preparation of Electrochemical Immunosensor to Detect Serum Amyloid A

Contact Info

Product

Resources

About