Fast method for identifying inter- and intra-species <i>Saccharomyces</i>
 hybrids in extensive genetic improvement programs based on yeast breeding

Solieri, Lisa; Verspohl, Alexandra; Bonciani, Tommaso; Caggia, Cinzia; Giudici, Paolo

doi:10.1111/jam.12827

Cited by 13 publications

(8 citation statements)

References 74 publications

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“…A major problem in direct mating programmes that apply spore‐to‐spore mating is the low yield of hybrids; this yield is even lower for interspecific hybrids than for intraspecific hybrids (Solieri et al, ). In this study, we obtained 83 hybrids from 754 crosses, resulting in a rate of 11% (Figure ).…”

Section: Resultsmentioning

confidence: 99%

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The inheritance of mitochondrial DNA in interspecific Saccharomyces hybrids and their properties in winemaking

Verspohl

Pignedoli

Giudici

2017

Yeast

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The inheritance of mitochondrial DNA (mtDNA) in yeast hybrids is heteroplasmic, turning into homoplasmic after few cell cycles. Mitotype restoration is reported to be biparental and random. This study shows that mitotype restoration follows fixed schemata. We created isogenic, interspecific Saccharomyces cerevisiae × Saccharomyces uvarum hybrids through direct mating and analysed their mating frequency and mitotype. The mating frequency increased for most crosses in staggered mating. Mitotyping revealed that breeding lines with the same parental strain of S. cerevisiae and different parental strains of S. uvarum give rise to the same mitotype. According to our results, we postulate that the inheritance of mtDNA is dominated by one parental strain and that the superior mitotype is specific to each breeding line. Technological tests showed typical hybrid heterosis.

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Section: Resultsmentioning

confidence: 99%

“…Both colonies were isolated in the event that different morphologies were found after streaking. The isolated colonies were screened with a colony PCR method on the ITS1 or ITS2 region as described previously (Solieri et al, ). In cases with a positive result, we randomly isolated three more hybrids to verify that the mitotype was homogenous.…”

Section: Methodsmentioning

confidence: 99%

The inheritance of mitochondrial DNA in interspecific Saccharomyces hybrids and their properties in winemaking

Verspohl

Pignedoli

Giudici

2017

Yeast

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“…Although the mating frequency for crossings between S. cerevisiae and S. uvarum is low (Solieri et al . ); this limit can be reduced by applying the technique of staggered mating, which results in higher hybridation yields (Verspohl et al . ).…”

Section: Resultsmentioning

confidence: 99%

Qualitative and quantitative screening of theβ-glucosidase activity inSaccharomyces cerevisiaeandSaccharomyces uvarumstrains isolated from refrigerated must

Bonciani

Vero

Giannuzzi

et al. 2018

Lett Appl Microbiol

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In yeasts, β-glucosidase activity has been extensively described, especially in non-Saccharomyces species, while there is only little evidence of this activity in strains belonging to the Saccharomyces species. In winemaking, β-glucosidase plays essential roles in the hydrolysis of glyco-conjugated precursors and the release of active aromatic compounds. This study provides new insights into the β-glucosidase activity in strains belonging to Saccharomyces cerevisiae and Saccharomyces uvarum species, which are the most important strains in wine industry. Our results point out a marked enzymatic activity for the tested S. uvarum strains. These strains could be exploited for their potential ability to enhance the aroma profiles of wine. In addition, they could be potential sources for the commercial production of enzymes to be applied in winemaking.

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“…Then, mating is performed among the different obtained lines. Such a strategy allows a more thorough exploration of the phenotypic space, although suffering from an overall lower time-efficiency [50] compared to “direct mating”, which is thus the most suitable for the rapid improvement of traits associated to quantitative trait loci (QTLs). In any case, genetic improvement programs based on monosporic cultures should comprise the extensive screening of desired phenotypic and genotypic strains in the progenitors [51].…”

Section: Genetic Strategies To Improve Glutathione Production In Yeastsmentioning

confidence: 99%

High-glutathione producing yeasts obtained by genetic improvement strategies: a focus on adaptive evolution approaches for novel wine strains

et al. 2017

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Glutathione (GSH) is the most abundant non-protein thiol in living organisms. Due to its\ud important antioxidant role, it is widely used in medicine, as a food additive, and in the cosmetic\ud industry. Recently, GSH has received growing attention in winemaking because of its ability to\ud control oxidative spoilage damage and to protect various aromatic compounds. Indeed, GSH\ud concentration in wine is highly variable and several factors are involved in its regulation, ranging\ud from grape must to yeast fermentation activity. This short review aims at highlighting the common\ud genetic strategies, useful for obtaining wine yeasts with enhanced GSH production, paying particular\ud attention to the adaptive evolution approaches. Moreover, other strategies, such as random\ud mutagenesis, metabolic engineering and hybridization have been briefly reviewed with a stress on\ud both their strengths and weaknesses in terms of actual feasibility and acceptance by wine consumers

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Fast method for identifying inter- and intra-species Saccharomyces hybrids in extensive genetic improvement programs based on yeast breeding

Abstract: The study provides a method for the marker-assisted selection of several inter- and intra-species yeast hybrids in a cost-effective, rapid and reproducible manner.

Cited by 13 publications

References 74 publications

The inheritance of mitochondrial DNA in interspecific Saccharomyces hybrids and their properties in winemaking

The inheritance of mitochondrial DNA in interspecific Saccharomyces hybrids and their properties in winemaking

Qualitative and quantitative screening of theβ-glucosidase activity inSaccharomyces cerevisiaeandSaccharomyces uvarumstrains isolated from refrigerated must

High-glutathione producing yeasts obtained by genetic improvement strategies: a focus on adaptive evolution approaches for novel wine strains

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