“…Tested parameters were NO 3 - , SO 4 2- , O 2 , Cl - , Na + , Mn (total) , Fe (total) , Br - , Mg 2+ , K + , Ca 2+ , Cu 2+ , Zn 2+ , PO 4 2- , and total organic carbon concentrations, temperature, conductivity, redox potential, pH, groundwater residence time, and depth. To address more directly how environmental parameters and individual OTUs were related, we performed a regularized Canonical Correlation Analysis (rCCA) between relative frequencies of OTUs and environmental parameters with the R package FRCC ( Cruz-Cano and Lee, 2014 ). We visualized results of the rCCA analysis using the approach developed by Patel et al (2010) A Network was built from the rCCA results.…”