2012
DOI: 10.1002/jssc.201200339
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Fast separations of intact proteins by liquid chromatography

Abstract: Scientists working in many fields require fast separations of intact proteins using liquid chromatography. The fast separations here concern not only the separation step alone but also the complete chromatographic process, including column regeneration, system equilibration, and buffer exchange, in one- and two-dimensional liquid chromatography in addition to fast purification technologies predominantly on the analytical scale with some unique examples on the preparative and industrial scales. This comprehensi… Show more

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Cited by 24 publications
(16 citation statements)
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“…Since the resolution and selectivity of this MMC column were comparable to the conventional IEC and HIC columns, and it also had much higher bioactivity recovery and mass recovery for protein under both HIC and WCX modes, the single MMC column could replace two individual IEC and HIC columns to separate proteins in both WCX and HIC modes. In addition, as HIC and WCX modes are orthorhombic, this dual-retention mechanism MMC column can be called a '2D column' Wang et al, 2012), which can be used to set up 2DLC with a single '2D column' as 2DLC-1C.…”
Section: The Reproducibility Of the Wcx/hic Dual-retention Mechanism mentioning
confidence: 99%
See 1 more Smart Citation
“…Since the resolution and selectivity of this MMC column were comparable to the conventional IEC and HIC columns, and it also had much higher bioactivity recovery and mass recovery for protein under both HIC and WCX modes, the single MMC column could replace two individual IEC and HIC columns to separate proteins in both WCX and HIC modes. In addition, as HIC and WCX modes are orthorhombic, this dual-retention mechanism MMC column can be called a '2D column' Wang et al, 2012), which can be used to set up 2DLC with a single '2D column' as 2DLC-1C.…”
Section: The Reproducibility Of the Wcx/hic Dual-retention Mechanism mentioning
confidence: 99%
“…Recently, our group synthesized a novel IEC/HIC (Zhao et al, 2012) dual-retention mechanism MMC column with the ligand containing the ion-exchange and hydrophobic groups. A column was reported to separate proteins with a single column in IEC and HIC modes, which was called a two-dimensional column (2D column; Geng et al, 2009;Wang et al, 2012). Later, Yang et al (2013) employed the 2D column for on-line fast separation and purification of cytochrome c (Cyt C) from bovine pancreas.…”
Section: Introductionmentioning
confidence: 99%
“…Intact proteins have been traditionally analyzed on conventional C 18 packed silica particle columns; however, due to the complex structure of biomolecules, many issues have limited the kinetic performances such as the low diffusion coefficient, the irreversible adsorption onto column, the secondary ionic interactions and/or the high backpressure values [12,13]. Compared with silica-based stationary phases, protein recovery from monolithic columns are certainly superior.…”
Section: Introductionmentioning
confidence: 99%
“…20 However, the separation speed depends on the complexity of the sample. 2,[21][22][23][24][25][26][27][28][29] This choice is particularly important in developing proteomics in which intact proteins are fragmented directly in the mass spectrometer to achieve both protein identification and characterization, even capturing information on combinatorial post-translational modifications. 2,[21][22][23][24][25][26][27][28][29] This choice is particularly important in developing proteomics in which intact proteins are fragmented directly in the mass spectrometer to achieve both protein identification and characterization, even capturing information on combinatorial post-translational modifications.…”
Section: Introductionmentioning
confidence: 99%