Fast simultaneous LC/MS/MS determination of 10 active compounds in human serum for therapeutic drug monitoring in psychiatric medication

Sistik, P.; Urinovska, R.; Brozmanová, Hana; Kacířová, Ivana; Šilhán, Petr; Lemr, Karel

doi:10.1002/bmc.3538

Cited by 23 publications

(10 citation statements)

References 35 publications

(61 reference statements)

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“…To the best of our knowledge, just one paper has been published on SNP chiral separation, but using CE with unmodified β‐cyclodextrin and without biological application . On the other hand, some analytical methods have been published for the non‐enantioselective determination of SNP in biological matrices among which those designed for patient monitoring are very few and only one dealing with micromatrices . None of them was applied to real samples from psychiatric patients, nor have they compared different and novel dried microsampling approaches.…”

Section: Introductionmentioning

confidence: 99%

Enantioseparation and determination of asenapine in biological fluid micromatrices by HPLC with diode array detection

Protti

Vignali

Blanco

et al. 2018

J of Separation Science

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Asenapine is a recent drug approved in the European Union for the treatment of bipolar disorder. An original approach has been developed for asenapine analysis in patients treated with the drug, including miniaturized microsampling procedures, separation and quantitation of drug enantiomers. An original enantioselective method based on high-performance liquid chromatography with diode array detection was developed and applied to the determination of asenapine enantiomer levels in innovative haematic samples: four micromatrices have been tested, two based on dried matrix spots (dried blood spots and dried plasma spots) and two based on volumetric absorptive microsampling (from blood and plasma). Chiral separation was achieved on a cellulose-tris(3,5 dimethylphenylcarbamate) column, with a mobile phase containing bicarbonate buffer and acetonitrile. The method was validated with satisfactory results of linearity and precision on all matrices that showed also a significant performance in terms of stability, feasibility and reliability, when compared to fluid plasma sampling, handling and processing. Among micromatrices, both volumetric absorptive microsampling types were superior to dried matrix spots in terms of data reproducibility and correspondence with plasma levels. The bioanalytical approach proposed herein provides for the first time a chiral high-performance liquid chromatographic method for the determination of asenapine enantiomers, coupled to a very effective microsampling strategy.

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Section: Introductionmentioning

confidence: 99%

Enantioseparation and determination of asenapine in biological fluid micromatrices by HPLC with diode array detection

Protti

Vignali

Blanco

et al. 2018

J of Separation Science

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“…To date, the lowest LLOQs were obtained by Patel and co-workers [34] with the value of 0.05 ng/ml for ARI and by Song and colleagues [23] with the value of 0.01 ng/ml for DARI. While not reaching the before-mentioned values, we still outperformed other UPLC-and LC-MS/MS methods developed by different authors [20,[24][25][26][27][28][29][30][31][32]32,33,38]. Table 2 summarizes the results for precision and accuracy with standard deviation (SD), CV and accuracy calculated for each sample.…”

Section: Calibration Curve Selectivity and Lloqmentioning

confidence: 79%

“…Moreover, in spite of large impact of matrix effects in LC-MS/MS analysis [41,42], it has not been taken into account in some of described methods [19][20][21]. Usually matrix effects' evaluation is based on post-column infusion method [24,28,31,38,43] or post-extraction addition method [30,41] Table 4 summarizes the results of the stability assays at low and high QC for ARI (0.5 and 110 ng/ml) and DARI (0.7 and 90 ng/ml). The stability of both analytes after 24…”

Section: Extraction Recovery Matrix Effect and Phospholipids Removalmentioning

confidence: 99%

“…Nonetheless, the majority of the methods of LC-MS/MS and ultra-LC-MS/MS (UPLC-MS/MS) with ESI have used protein precipitation (PPT) or liquid liquid extraction (LLE) in addition to non IL-IS instead of solid phase extraction (SPE) and stable IL-IS [19][20][21][21][22][23][24][25][26][27][28][29][30][31]. PPT is the fastest, but the least effective sample preparation technique, often resulting in significant matrix effects due mainly to the presence of endogenous phospholipidss [17].…”

Section: Introductionmentioning

confidence: 99%

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Effective phospholipids removing microelution-solid phase extraction LC-MS/MS method for simultaneous plasma quantification of aripiprazole and dehydro-aripiprazole: Application to human pharmacokinetic studies

Wojnicz

Belmonte

Koller

et al. 2018

Journal of Pharmaceutical and Biomedical Analysis

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A simple liquid chromatography-tandem mass spectrometry (LC-MS/MS) method has been developed and validated for simultaneous quantification of aripiprazole and its active metabolite dehydro-aripiprazole in human plasma. Isotopically labeled aripiprazole, aripiprazole-D8, has been used as the internal standard (IS) for both analytes. Only 200 µl of human plasma was needed for analyte extraction, using effective phospholipids-eliminating three-steps microelution-solid-phase extraction (SPE, Oasis PRiME HLB 96-well µElution Plate). An ACE C18-PFP column was applied for chromatographic separation at 25°C, protected by a 0.2-μm on-line filter. A combination of ammonium formate (5 mM)-acetonitrile (pH 4.0; 65:35, v/v) was used as mobile phase and the chromatogram was run under gradient conditions at a flow rate of 0.6 ml/min. Run time lasted for 5 minutes-followed by a re-equilibration time of 3 minutes, to give a total run time of 8 minutes. 5 μl of the sample was injected into the chromatographic system. Aripiprazole, dehydro-aripiprazole and IS were detected using the mode multiple reaction monitoring in the positive ionization mode. The method was linear in the concentration range of 0.18-110 ng/ml and 0.35-100 ng/ml for ARI and DARI, respectively. Our method has been validated according to the recommendations of regulatory agencies through tests of precision, accuracy, recovery, matrix effect, stability, sensitivity, selectivity and carry-over. Our microelution-SPE method removes more than 99% of main plasma phospholipids compared to protein precipitation and was successfully applied to several bioequivalence studies.

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“…Currently, a number of advanced quantitative technologies-high-performance liquid chromatography, liquid chromatography tandem-mass spectrometry (LC-MS/MS), and liquid chromatography-electrospray ionization tandem-mass spectrometry (LC-ESI-MS/MS)-are being widely used as analysis methods in research on biological mechanisms. [1][2][3][4][5][6] However, although LC-MS is a highly sensitive and fast method for profiling compounds, it does not provide information on the spatial distribution of target analytes on the sample surface.…”

Section: Introductionmentioning

confidence: 99%

Comparison between thaw-mounting and use of conductive tape for sample preparation in ToF-SIMS imaging of lipids in Drosophila microRNA-14 model

Son

Shon

et al. 2018

Biointerphases

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Time-of-flight secondary ion mass spectrometry (ToF-SIMS) imaging elucidates molecular distributions in tissue sections, providing useful information about the metabolic pathways linked to diseases. However, delocalization of the analytes and inadequate tissue adherence during sample preparation are among some of the unfortunate phenomena associated with this technique due to their role in the reduction of the quality, reliability, and spatial resolution of the ToF-SIMS images. For these reasons, ToF-SIMS imaging requires a more rigorous sample preparation method in order to preserve the natural state of the tissues. The traditional thaw-mounting method is particularly vulnerable to altered distributions of the analytes due to thermal effects, as well as to tissue shrinkage. In the present study, the authors made comparisons of different tissue mounting methods, including the thaw-mounting method. The authors used conductive tape as the tissue-mounting material on the substrate because it does not require heat from the finger for the tissue section to adhere to the substrate and can reduce charge accumulation during data acquisition. With the conductive-tape sampling method, they were able to acquire reproducible tissue sections and high-quality images without redistribution of the molecules. Also, the authors were successful in preserving the natural states and chemical distributions of the different components of fat metabolites such as diacylglycerol and fatty acids by using the tape-supported sampling in microRNA-14 (miR-14) deleted Drosophila models. The method highlighted here shows an improvement in the accuracy of mass spectrometric imaging of tissue samples.

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Fast simultaneous LC/MS/MS determination of 10 active compounds in human serum for therapeutic drug monitoring in psychiatric medication

Cited by 23 publications

References 35 publications

Enantioseparation and determination of asenapine in biological fluid micromatrices by HPLC with diode array detection

Enantioseparation and determination of asenapine in biological fluid micromatrices by HPLC with diode array detection

Effective phospholipids removing microelution-solid phase extraction LC-MS/MS method for simultaneous plasma quantification of aripiprazole and dehydro-aripiprazole: Application to human pharmacokinetic studies

Comparison between thaw-mounting and use of conductive tape for sample preparation in ToF-SIMS imaging of lipids in Drosophila microRNA-14 model

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