Fast Stiffness Mapping of Cells Using High-Bandwidth Atomic Force Microscopy

Wang, Andrew; Vijayraghavan, K.; Solgaard, Olav; Butte, Manish J.

doi:10.1021/acsnano.5b03959

Cited by 24 publications

(25 citation statements)

References 42 publications

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“…Here, we present a design of the force volume AFM experiment, aimed to perform a fast tissue characterization with a standard, non-high-speed AFM setup 6,8,[18][19][20][21][22] . In our experiments, about 30 min are required for characterizing one tissue section, i.e.for the tip positioning in relevant areas of the sample and the mapping of 10 areas of the section, 5 for CE and 5 for CL locations.…”

Section: Discussionmentioning

confidence: 99%

Spatial mapping of the collagen distribution in human and mouse tissues by force volume atomic force microscopy

Calò

Romin

Srouji

et al. 2020

Sci Rep

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Changes in the elastic properties of living tissues during normal development and in pathological processes are often due to modifications of the collagen component of the extracellular matrix at various length scales. Force volume AFM can precisely capture the mechanical properties of biological samples with force sensitivity and spatial resolution. The integration of AFM data with data of the molecular composition contributes to understanding the interplay between tissue biochemistry, organization and function. The detection of micrometer-size, heterogeneous domains at different elastic moduli in tissue sections by AFM has remained elusive so far, due to the lack of correlations with histological, optical and biochemical assessments. In this work, force volume AFM is used to identify collagen-enriched domains, naturally present in human and mouse tissues, by their elastic modulus. Collagen identification is obtained in a robust way and affordable timescales, through an optimal design of the sample preparation method and AFM parameters for faster scan with micrometer resolution. The choice of a separate reference sample stained for collagen allows correlating elastic modulus with collagen amount and position with high statistical significance. The proposed preparation method ensures safe handling of the tissue sections guarantees the preservation of their micromechanical characteristics over time and makes it much easier to perform correlation experiments with different biomarkers independently.

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Section: Discussionmentioning

confidence: 99%

Spatial mapping of the collagen distribution in human and mouse tissues by force volume atomic force microscopy

Calò

Romin

Srouji

et al. 2020

Sci Rep

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“…Since the atomic force microscope (AFM) was introduced in 1986 [ 1 ], it has been an important tool in nanotechnology to simultaneously achieve high-resolution topography imaging [ 2 , 3 ] and quantify the physical properties of samples [ 4 , 5 ] in various environments. For example, the AFM can be used to image and manipulate biological samples on atomic even submicroscopic scales in buffer solution [ 6 ] and explore the nature of the ionic liquids [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

A High-Q AFM Sensor Using a Balanced Trolling Quartz Tuning Fork in the Liquid

Zhang

Song

et al. 2018

Sensors

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A quartz tuning fork (QTF) has been widely used as a force sensor of the frequency modulation atomic force microscope due to its ultrahigh stiffness, high quality factor and self-sensing nature. However, due to the bulky structure and exposed surface electrode arrangement, its application is limited, especially in liquid imaging of in situ biological samples, ionic liquids, electrochemical reaction, etc. Although the complication can be resolved by coating insulating materials on the QTF surface and then immersing the whole QTF into the liquid, it would result in a sharp drop of the quality factor, which will reduce the sensitivity of the QTF. To solve the problem, a novel method, called the balanced trolling quartz tuning fork (BT-QTF), is introduced here. In this method, two same probes are glued on both prongs of the QTF separately while only one probe immersed in the liquid. With the method, the hydrodynamic interaction can be reduced, thus the BT-QTF can retain a high quality factor and constant resonance frequency. The stable small vibration of the BT-QTF can be achieved in the liquid. Initially, a theoretical model is presented to analyze the sensing performance of the BT-QTF in the liquid. Then, the sensing performance analysis experiments of the BT-QTF have been performed. At last, the proposed method is applied to atomic force microscope imaging different samples in the liquid, which proves its feasibility.

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“…roughness, height, friction and surface rheology), 31 molecular biology (e.g. DNA and chromosome studies and monitoring biological processes), 32,33 cell biology (cell imaging & mechanical properties) 34 and medicine (e.g. virology and tissue/organ imaging).…”

Section: Introductionmentioning

confidence: 99%