Plant phenotyping has been recognized as a bottleneck for improving the efficiency of breeding programs, understanding plant-environment interactions, and managing agricultural systems. In the past five years, imaging approaches have shown great potential for high-throughput plant phenotyping, resulting in more attention paid to imaging-based plant phenotyping. With this increased amount of image data, it has become urgent to develop robust analytical tools that can extract phenotypic traits accurately and rapidly. The goal of this review is to provide a comprehensive overview of the latest studies using deep convolutional neural networks (CNNs) in plant phenotyping applications. We specifically review the use of various CNN architecture for plant stress evaluation, plant development, and postharvest quality assessment. We systematically organize the studies based on technical developments resulting from imaging classification, object detection, and image segmentation, thereby identifying state-of-the-art solutions for certain phenotyping applications. Finally, we provide several directions for future research in the use of CNN architecture for plant phenotyping purposes.