Fasting and refeeding modulate neutral amino acid transport activity in the basolateral membrane of the rat exocrine pancreatic epithelium: fasting-induced insulin insensitivity

Mann, Giovanni E.; Muñoz, Manuel J.; Peran, Suzana

doi:10.1016/0005-2736(86)90475-x

Cited by 12 publications

(2 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, it suggests that changes in dietary composition induce selective adaptive responses in the transport of the different AAs to the pancreas, as has been described previously. 109 , 110 , 111 , 112 , 113 , 114 , 115 Interestingly, glycine levels increase (approximately double) in the 0% Prot and No Leu groups ( Table 2 ).…”

Section: Discussionmentioning

confidence: 94%

Dietary Protein and Amino Acid Deficiency Inhibit Pancreatic Digestive Enzyme mRNA Translation by Multiple Mechanisms

Sans

Crozier

Vogel

et al. 2021

Cellular and Molecular Gastroenterology and Hepatology

View full text Add to dashboard Cite

Background & Aims Chronic amino acid (AA) deficiency, as in kwashiorkor, reduces the size of the pancreas through an effect on mammalian target of rapamycin complex 1 (mTORC1). Because of the physiological importance of AAs and their role as a substrate, a stimulant of mTORC1, and protein synthesis, we studied the effect of acute protein and AA deficiency on the response to feeding. Methods ICR/CD-1 mice were fasted overnight and refed for 2 hours with 4 different isocaloric diets: control (20% Prot); Protein-free (0% Prot); control (AA-based diet), and a leucine-free (No Leu). Protein synthesis, polysomal profiling, and the activation of several protein translation factors were analyzed in pancreas samples. Results All diets stimulated the Protein Kinase-B (Akt)/mTORC1 pathway, increasing the phosphorylation of the kinase Akt, the ribosomal protein S6 (S6) and the formation of the eukaryotic initiation factor 4F (eIF4F) complex. Total protein synthesis and polysome formation were inhibited in the 0% Prot and No Leu groups to a similar extent, compared with the 20% Prot group. The 0% Prot diet partially reduced the Akt/mTORC1 pathway and the activity of the guanine nucleotide exchange factor eIF2B, without affecting eIF2α phosphorylation. The No Leu diet increased the phosphorylation of eIF2α and general control nonderepressible 2, and also inhibited eIF2B activity, without affecting mTORC1. Essential and nonessential AA levels in plasma and pancreas indicated a complex regulation of their cellular transport mechanisms and their specific effect on the synthesis of digestive enzymes. Conclusions These studies show that dietary AAs are important regulators of postprandial digestive enzyme synthesis, and their deficiency could induce pancreatic insufficiency and malnutrition.

show abstract

Section: Discussionmentioning

confidence: 94%

Dietary Protein and Amino Acid Deficiency Inhibit Pancreatic Digestive Enzyme mRNA Translation by Multiple Mechanisms

Sans

Crozier

Vogel

et al. 2021

Cellular and Molecular Gastroenterology and Hepatology

View full text Add to dashboard Cite

show abstract

“…In a previous study we reported that pancreatic L-phenylalanine transport was saturable (Kt = 10 mm, Vmax = 7 jtmol/min. g) and modulated by fasting and reseeding (Mann et al 1986). We have now investigated whether transport is rate-limiting for exocrine protein synthesis in perfused pancreata isolated from anaesthetized (60 mg/kg Sagatal, i.P.)…”

Section: W8 7ahmentioning

confidence: 99%

Proceedings of the Physiological Society, 15‐16 December 1989, King's College London Meeting: Communications

1990

The Journal of Physiology

View full text Add to dashboard Cite

Application of Rapid Dual Tracer Dilution Techniques for the Study of Endothelial Cell Amino Acid Transport in Perfused Microcarrier Cultures

Mann

Sheriff

Toothill

et al. 1991

Cell Membrane Transport

View full text Add to dashboard Cite

Fasting and refeeding modulate neutral amino acid transport activity in the basolateral membrane of the rat exocrine pancreatic epithelium: fasting-induced insulin insensitivity

Cited by 12 publications

References 17 publications

Dietary Protein and Amino Acid Deficiency Inhibit Pancreatic Digestive Enzyme mRNA Translation by Multiple Mechanisms

Dietary Protein and Amino Acid Deficiency Inhibit Pancreatic Digestive Enzyme mRNA Translation by Multiple Mechanisms

Proceedings of the Physiological Society, 15‐16 December 1989, King's College London Meeting: Communications

Application of Rapid Dual Tracer Dilution Techniques for the Study of Endothelial Cell Amino Acid Transport in Perfused Microcarrier Cultures

Contact Info

Product

Resources

About