Fasting induced kisspeptin signaling suppression is regulated by glutamate mediated cues in adult male rhesus macaque (Macaca mulatta)

Shamas, Shazia; Khan, Salman; Khan, Muhammad Yousaf; Shabbir, Nadia; Zubair, Hira; Shafqat, Saira; Wahab, Fazal; Shahab, Muhammad

doi:10.1016/j.npep.2015.06.005

Cited by 15 publications

(7 citation statements)

References 45 publications

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“…One of the key stimulants is glutamate, the main excitatory neurotransmitter in the brain, while GABA has an inhibitory effect (Clarkson et al, 2006;Watanabe et al, 2014). Studies conducted on monkeys confirmed the opposite role of these two neurotransmitters, showing that GABA levels gradually decrease before puberty, while glutamate levels are high at the beginning of puberty, before GnRH secretion (Shamas et al, 2015;Brann et al, 2002;Kurian et al, 2012). In particular, Kurian and co-authors demonstrated that an infusion of a GABAa receptor antagonist (bicuculline) within the stalk-median eminence of pre-pubertal monkeys could cause an increased release of kisspeptin, and consequently of GnRH secretion.…”

Section: Onset Of Puberty: the Gnrh Pulse Generator The Kndy System Excitatory And Inhibitory Neuromodulatorsmentioning

confidence: 99%

Hypothalamo-Pituitary axis and puberty

Spaziani

Tarantino

Tahani

et al. 2021

Molecular and Cellular Endocrinology

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Section: Onset Of Puberty: the Gnrh Pulse Generator The Kndy System Excitatory And Inhibitory Neuromodulatorsmentioning

confidence: 99%

Hypothalamo-Pituitary axis and puberty

Spaziani

Tarantino

Tahani

et al. 2021

Molecular and Cellular Endocrinology

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“…These neurons have been documented to contain receptors for metabolic hormones, and their activities are modulated by metabolic cues ( 44 , 67 , 92 – 94 ). In a recent study, we checked changes in the hypothalamic glutamate and GABA systems in fed and 48 hours fasted monkeys via checking transcripts levels of Kiss1, Kiss1r, NR1 ( N -methyl- d -aspartate receptor subunit 1) and GAD67 (glutamic acid decarboxylase67) in the mediobasal hypothalamus (MBH) and pre-optic area (POA) of the adult male rhesus macaque ( Macaca mulatta ) ( 95 ). The expression of Kiss1, Kiss1r , and NR1 mRNA was greatly decreased in fasted macaques as compared to ad libitum fed monkeys.…”

Section: Mechanism Of Metabolic Impact On the Hypothalamic Kp Systemmentioning

confidence: 99%

“…Taken together, these observations indicate that a reduction in inputs of glutamate-containing neurons to KPergic neurons may be responsible for the reduction in the hypothalamic KP signaling in the fasted monkey. However, no obvious change in expression of GAD67 mRNA between fed and fasted monkey was observed, suggesting that the fasting-induced reduction in the hypothalamic KP signaling is not mediated through GABAergic neurons ( 95 ).…”

Section: Mechanism Of Metabolic Impact On the Hypothalamic Kp Systemmentioning

confidence: 99%

Metabolic Impact on the Hypothalamic Kisspeptin-Kiss1r Signaling Pathway

et al. 2018

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A large body of data has established the hypothalamic kisspeptin (KP) and its receptor, KISS1R, as major players in the activation of the neuroendocrine reproductive axis at the time of puberty and maintenance of reproductive capacity in the adult. Due to its strategic location, this ligand-receptor pair acts as an integrator of cues from gonadal steroids as well as of circadian and seasonal variation-related information on the reproductive axis. Besides these cues, the activity of the hypothalamic KP signaling is very sensitive to the current metabolic status of the body. In conditions of energy imbalance, either positive or negative, a number of alterations in the hypothalamic KP signaling pathway have been documented in different mammalian models including nonhuman primates and human. Deficiency of metabolic fuels during fasting causes a marked reduction of Kiss1 gene transcript levels in the hypothalamus and, hence, decreases the output of KP-containing neurons. Food intake or exogenous supply of metabolic cues, such as leptin, reverses metabolic insufficiency-related changes in the hypothalamic KP signaling. Likewise, alterations in Kiss1 expression have also been reported in other situations of energy imbalance like diabetes and obesity. Information related to the body’s current metabolic status reaches to KP neurons both directly as well as indirectly via a complex network of other neurons. In this review article, we have provided an updated summary of the available literature on the regulation of the hypothalamic KP-Kiss1r signaling by metabolic cues. In particular, the potential mechanisms of metabolic impact on the hypothalamic KP-Kiss1r signaling, in light of available evidence, are discussed.

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“…97 In sheep, KISS1 cells send projections to, and receive contacts from, both Npy/AgRP and Pomc/Cart neurons. 92,98 Whether the impact of food-related cues be direct or indirect, through Npy/AgRP and/or Pomc/Cart neurons, fasting has been found to potently inhibit Kiss1 expression in the ARC of rats, [99][100][101][102][103] mice, 104 macaque, 105,106 and also decreases Kiss1 mRNA and KISS1 protein levels in the ARC of sheep, 92,[107][108][109][110] even though several studies failed to find an impact of FRes upon ARC Kiss1 mRNA levels in rats, 111 mice 112 or gilts. 113 Our data show a very minor (~12%), non-statistically-significant decrease in Kiss1 mRNA levels in FRes ewes, which contrasts with prior findings in this species.…”

Section: Discussionmentioning

confidence: 99%

Impact of food restriction on the medio‐basal hypothalamus of intact ewes as revealed by a large‐scale transcriptomics study

Dardente

Lomet

Desmarchais

et al. 2022

J Neuroendocrinology

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In mammals, the medio-basal hypothalamus (MBH) integrates photoperiodic and food-related cues to ensure timely phasing of physiological functions, including seasonal reproduction. The current human epidemics of obesity and associated reproductive disorders exemplifies the tight link between metabolism and reproduction.Yet, how food-related cues impact breeding at the level of the MBH remains unclear.In this respect, the sheep, which is a large diurnal mammal with a marked dual photoperiodic/metabolic control of seasonal breeding, is a relevant model. Here, we present a large-scale study in ewes (n = 120), which investigated the impact of food restriction (FRes) on the MBH transcriptome using unbiased RNAseq, followed by RT-qPCR. Few genes (~100) were impacted by FRes and the transcriptional impact was very modest (<2-fold increase or < 50% decrease for most genes). As anticipated, FRes increased expression of Npy/AgRP/LepR and decreased expression of Pomc/Cartpt, while Kiss1 expression was not impacted. Of particular interest, Eya3, Nmu and Dio2, genes involved in photoperiodic decoding within the MBH, were also affected by FRes. Finally, we also identified a handful of genes not known to be regulated by food-related cues (e.g., RNase6, HspA6, Arrdc2). In conclusion, our transcriptomics study provides insights into the impact of metabolism on the MBH in sheep, which may be relevant to human, and identifies possible molecular links between metabolism and (seasonal) reproduction.

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Fasting induced kisspeptin signaling suppression is regulated by glutamate mediated cues in adult male rhesus macaque (Macaca mulatta)

Cited by 15 publications

References 45 publications

Hypothalamo-Pituitary axis and puberty

Hypothalamo-Pituitary axis and puberty

Metabolic Impact on the Hypothalamic Kisspeptin-Kiss1r Signaling Pathway

Impact of food restriction on the medio‐basal hypothalamus of intact ewes as revealed by a large‐scale transcriptomics study

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