2011
DOI: 10.1507/endocrj.k11e-131
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Fasting reduces the kiss1 mRNA levels in the caudal hypothalamus of gonadally intact adult female rats

Abstract: Kisspeptin, which is the product of the kiss1 gene and its receptor kiss1r, have emerged as the essential gatekeepers of reproduction. The present study used gonadally intact female rats to evaluate fasting-induced suppression of the KiSS-1 system of anteroventral periventricular nucleus (AVPV) and arcuate nucleus (ARC) under normal physiological conditions. Starting on the day of estrous, one group of rats was subjected to 72 h of food deprivation, while the other group of rats was able to continue feeding a… Show more

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Cited by 51 publications
(56 citation statements)
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“…The PCR conditions were as follows: initial denaturation and enzyme activation at 95 °C for 20 s, followed by 45 cycles of denaturation at 95 °C for 3 s and annealing at 65 °C for 30 s (Kiss1) or 64 °C for 30 s (GAPDH), and then extension at 72 °C for 1 min. The copy numbers of the transcripts were normalized against those of GAPDH transcripts for each sample [18,19].…”
Section: Real Time Reverse-transcription Pcr Analysismentioning
confidence: 99%
See 1 more Smart Citation
“…The PCR conditions were as follows: initial denaturation and enzyme activation at 95 °C for 20 s, followed by 45 cycles of denaturation at 95 °C for 3 s and annealing at 65 °C for 30 s (Kiss1) or 64 °C for 30 s (GAPDH), and then extension at 72 °C for 1 min. The copy numbers of the transcripts were normalized against those of GAPDH transcripts for each sample [18,19].…”
Section: Real Time Reverse-transcription Pcr Analysismentioning
confidence: 99%
“…These sampling were done on diestrus day in control rats during 12 weeks of age. Anterior and posterior hypothalamic tissue blocks were cut as reported previously and based on rat brain atlas [17,18]. In this cutting method, the anteroventral periventricular nucleus (AVPV) and the arcuate nucleus (ARC) are included in the anterior and posterior hypothalamic blocks, respectively.…”
mentioning
confidence: 99%
“…Energydeprived rats and sheep had reduced Kiss1 mRNA in their hypothalami, reduced LH levels and pubertal arrest. (47,(124)(125)(126)(127)(128)(129)(130)(131) With kisspeptin administration, puberty resumed, while gonadotrophin and androgen levels normalised. (57,124,125) Interactions between kisspeptins and leptin may account for these observations.…”
Section: Kisspeptins and Energy Homeostasismentioning
confidence: 99%
“…They also have less body fat and low serum leptin concentration. In rodent, fasting reduces the kiss1 mRNA expression level in ARC and serum LH concentration and prolonged estrous cycle [17]. In this study, we tried to determine whether stimulation of NKB/neurokinin 3 receptor (NK3R) signaling and inhibition of Dyn/kappa-opioid receptor (KOR) signaling recover LH secretion suppressed by negative energy in male rats in order to evaluate the efficacy of such drugs in the treatment for the ovulatory disorder.…”
Section: Originalmentioning
confidence: 99%