Fatal Amebiasis: Report of 148 Fatal Cases From the Armed Forces Institutes of Pathology

Kean, B. H.; Gilmore, Hugh R.; Stone, William W. Van

doi:10.7326/0003-4819-44-5-831

Cited by 51 publications

(9 citation statements)

References 11 publications

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“…The total lysate of HA-EhGEF expressing trophozoites were incubated with PI lipid array and probed with antibodies specific to HA tag. The results showed that the HA-GEF was able to bind PtdIns (3,4,5)P3 and some cross reactivity was also observed for PtdIns (3,4)P2 which is well documented in literature for this domain (Fig 1C). The HA-tagged PH domain alone (ΔEhGEF) also showed the same pattern of PI binding (Fig 1D).…”

Section: Ehgef Binds Phosphoinositides (Pi)supporting

confidence: 84%

“…The HA-tagged PH domain alone (ΔEhGEF) also showed the same pattern of PI binding (Fig 1D). The results indicate that PH domain of EhGEF is responsible for PtdIns (3,4,5)P3 binding in the plasma membrane and also localize the recruitment of EhRho1 to the site of phagocytosis.…”

Section: Ehgef Binds Phosphoinositides (Pi)mentioning

confidence: 84%

“…Following activation of EhRho1 by EhGEF, we focussed on involvement of EhGEF in recruitment of EhRho1 and its downstream partners, EhFormin1 and EhProfilin1 to the phagocytic cups. As PH domain of EhGEF binds PtdIns (3,4,5)P3 which is well known to be generated during endocytic processes, EhGEF might also play role in recruiting EhRho1 to phagocytic site. In order to demonstrate this, we visualized the sub-cellular localization of EhRho1, EhFormin1 and EhProfilin1 in RBC phagocytosing cells transfected with antisense constructs of EhGEF in the presence and absence of tetracycline.…”

Section: Plos Pathogensmentioning

confidence: 99%

“…Entamoeba histolytica is the causative agent of amoebic dysentery or amoebiasis, a major public health problem throughout the world, particularly in developing countries and is one of the major causes of morbidity and mortality [1,2]. The parasite can invade both intestinal as well as extra intestinal sites and in the absence of proper diagnosis or treatment, it can be fatal [3,4]. E. histolytica is a highly phagocytic cell, and phagocytosis plays a key role in amoebic pathogenesis.…”

Section: Introductionmentioning

confidence: 99%

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An atypical EhGEF regulates phagocytosis in Entamoeba histolytica through EhRho1

et al. 2021

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The parasite Entamoeba histolytica is the etiological agent of amoebiasis, a major cause of morbidity and mortality due to parasitic diseases in developing countries. Phagocytosis is an essential mode of obtaining nutrition and has been associated with the virulence behaviour of E. histolytica. Signalling pathways involved in activation of cytoskeletal dynamics required for phagocytosis remains to be elucidated in this parasite. Our group has been studying initiation of phagocytosis and formation of phagosomes in E. histolytica and have described some of the molecules that play key roles in the process. Here we showed the involvement of non-Dbl Rho Guanine Nucleotide Exchange Factor, EhGEF in regulation of amoebic phagocytosis by regulating activation of EhRho1. EhGEF was found in the phagocytic cups during the progression of cups, until closure of phagosomes, but not in the phagosomes themselves. Our observation from imaging, pull down experiments and down regulating expression of different molecules suggest that EhGEF interacts with EhRho1 and it is required during initiation of phagocytosis and phagosome formation. Also, biophysical, and computational analysis reveals that EhGEF mediates GTP exchange on EhRho1 via an unconventional pathway. In conclusion, we describe a non-Dbl EhGEF of EhRho1 which is involved in endocytic processes of E. histolytica.

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Section: Ehgef Binds Phosphoinositides (Pi)supporting

confidence: 84%

Section: Ehgef Binds Phosphoinositides (Pi)mentioning

confidence: 84%

Section: Plos Pathogensmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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An atypical EhGEF regulates phagocytosis in Entamoeba histolytica through EhRho1

et al. 2021

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“…If fulminant colitis develops, the outcome is poor with mortality ranging from 55% to 87.5% [17], peritonitis being the commonest cause of death [18]. Early diagnosis and surgical treatment significantly decrease mortality [8,9].…”

Section: Discussionmentioning

confidence: 99%

Acute fulminant necrotizing amoebic colitis: a rare and fatal complication of amoebiasis: a case report

Gupta

Singh

Shukla

et al. 2009

Cases J

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Acute Fulminant Necrotizing Amoebic Colitis is a rare complication of amoebiasis that is associated with high mortality. Only one to four such cases are seen per year in large hospitals of India, and only few such cases have been reported in the literature. The condition requires early diagnosis and surgical intervention. We recently cared for a patient who presented with acute abdomen with history of intermittent abdominal pain and diarrhea. Before presenting to our institution he was misdiagnosed as a case of inflammatory bowel disease and had been treated with steroids. On emergency exploration, extensive necrosis and multiple perforations in retroperitoneum involving entire colon were seen. Total colectomy with ileostomy was performed. Postoperative course was marked by septicaemia and multi-organ failure followed by death. This case report emphasizes the importance of early diagnosis and treatment of acute FAC, and associated high mortality.

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Infectious Diseases

Reimann¹

1957

AMA Arch Intern Med

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Fatal Amebiasis: Report of 148 Fatal Cases From the Armed Forces Institutes of Pathology

Cited by 51 publications

References 11 publications

An atypical EhGEF regulates phagocytosis in Entamoeba histolytica through EhRho1

An atypical EhGEF regulates phagocytosis in Entamoeba histolytica through EhRho1

Acute fulminant necrotizing amoebic colitis: a rare and fatal complication of amoebiasis: a case report

Infectious Diseases

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