Fatal Effects of a Neonatal High-Protein Diet in Low-Birth-Weight Piglets Used as a Model of Intrauterine Growth Restriction

Jamin, Agnès; d'Inca, Romain; Floc'H, Nathalie Le; Küster, Alice; Orsonneau, Jean-Luc; Darmaun, Dominique; Boudry, Gaëlle; Huërou‐Luron, Isabelle Le; Sève, Bernard; Guen, C. Gras-Le

doi:10.1159/000260135

Cited by 35 publications

(32 citation statements)

References 47 publications

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“…Thirty four cross-bred full-term IUGR piglets were obtained from the experimental herd of Institut National de la Recherche Agronomique (INRA, Saint-Gilles, France). They were chosen at birth, as described previously (25), so that their mean birth body weight (BW) was 0.92 Ϯ 0.02 kg (i.e., around the 10th percentile of the herd birth weight). Both entire male and female piglets were allowed to suckle colostrum from their dams for the first 2 days.…”

Section: Animals and Dietsmentioning

confidence: 99%

“…Pairs of littermates of the same sex were then randomly assigned to one of the two dietary groups. They received milk replacers formulated to provide an adequate protein (AP; 1.05 g of protein/100 kJ mimicking sow's milk) or a high protein (HP; 1.5 g of protein/100 kJ) protein supply (Table 1; 25). After separation from their dam, they were placed individually in incubators (33°C, 60% humidity) and were bottle-fed every 2 h from 0700 to 2300 and once during the night at 0300 from day 2 to day 7 (25).…”

Section: Animals and Dietsmentioning

confidence: 99%

“…They received milk replacers formulated to provide an adequate protein (AP; 1.05 g of protein/100 kJ mimicking sow's milk) or a high protein (HP; 1.5 g of protein/100 kJ) protein supply (Table 1; 25). After separation from their dam, they were placed individually in incubators (33°C, 60% humidity) and were bottle-fed every 2 h from 0700 to 2300 and once during the night at 0300 from day 2 to day 7 (25). At day 7, piglets were transferred into stainless-steel metabolic cages in a temperaturecontrolled room (30°C), and they were then fed with an automatic formula feeder (37) up to day 28.…”

Section: Animals and Dietsmentioning

confidence: 99%

“…The HP powder was formulated to provide more protein and amino acids per NE than the AP powder but with the same fat to carbohydrate ratio. A formula was prepared by mixing AP and HP powders (30/70 vol/vol) from day 2 to day 7 to offer 30% more proteins in HP piglets than in those fed AP powder (25). Then, HP piglets were fed reconstituted HP powder from day 8 onward, so that the amount of proteins received was 41% more in this group than in the AP group.…”

Section: Animals and Dietsmentioning

confidence: 99%

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A high-protein neonatal formula induces a temporary reduction of adiposity and changes later adipocyte physiology

Sarr

Gondret

Jamin

et al. 2011

American Journal of Physiology-Regulatory, Integrative and Comp

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Sarr O, Gondret F, Jamin A, Le Huërou-Luron I, Louveau I. A high-protein neonatal formula induces a temporary reduction of adiposity and changes later adipocyte physiology. Am J Physiol Regul Integr Comp Physiol 300: R387-R397, 2011. First published December 1, 2010; doi:10.1152/ajpregu.00459.2010.-The high-protein content of formula offered to low-birth weight babies is suspected to increase the risk of obesity later in life. This study assesses the immediate and subsequent effects of a protein intake in excess during suckling on hormonal and metabolic status and adipose tissue features in a porcine model of intrauterine growth restriction. Piglets were fed milk replacers formulated to provide an adequate (AP) or a high (HP) protein supply from day 2 to day 28. A subset of piglets was killed at day 28. After weaning, the remaining piglets had free access to the same solid high-fat diet until day 160. From day 2 to day 28, HP piglets had a greater daily weight gain (P Ͻ 0.05). Relative weight of perirenal adipose tissue (PAT), adipocyte mean diameters, activities of lipogenic enzymes in PAT and subcutaneous adipose tissue (SCAT), and leptinemia were lower (P Ͻ 0.05) in HP piglets than in AP piglets. Genes related to glucose utilization and lipid anabolism in PAT and SCAT were (P Ͻ 0.05) or tended (P Ͻ 0.1) to be downregulated in HP piglets. At day 160, adipocytes were enlarged, whereas lipogenic rates in adipocytes were reduced (P Ͻ 0.05) in SCAT of HP compared with AP pigs. Percent body fat, mRNA levels of genes controlling lipid metabolism, and plasma concentrations of hormones and metabolites were similar in HP and AP pigs. In conclusion, a HP neonatal formula induced a temporary reduction of adiposity and changed adipocyte physiology at peripubertal age.

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Section: Animals and Dietsmentioning

confidence: 99%

Section: Animals and Dietsmentioning

confidence: 99%

Section: Animals and Dietsmentioning

confidence: 99%

Section: Animals and Dietsmentioning

confidence: 99%

See 2 more Smart Citations

A high-protein neonatal formula induces a temporary reduction of adiposity and changes later adipocyte physiology

Sarr

Gondret

Jamin

et al. 2011

American Journal of Physiology-Regulatory, Integrative and Comp

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“…2008; Jamin et al. 2010). In a similar study design, HP feeding of IUGR piglets has been shown to induce metabolic and electrolyte disturbances mimicking refeeding syndrome, a surrogate of active anabolic response to HP intake (Jamin et al.…”

Section: Discussionmentioning

confidence: 99%

Neonatal high protein intake enhances neonatal growth without significant adverse renal effects in spontaneous IUGR piglets

Boubred

Jamin

Buffat³

et al. 2017

Physiol Rep

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In humans, early high protein (HP) intake has been recommended to prevent postnatal growth restriction and complications of intrauterine growth restriction (IUGR). However, the impact of such a strategy on the kidneys remains unknown, while significant renal hypertrophy, proteinuria, and glomerular sclerosis have been demonstrated in few experimental studies. The objective of this study was to evaluate the effects of a neonatal HP formula on renal structure in IUGR piglets. Spontaneous IUGR piglets were randomly allocated to normal protein (NP, n = 10) formula or to HP formula (+50% protein content, n = 10) up to day 28 after birth. Body weight, body composition, renal functions, and structure were assessed at the end of the neonatal period. While birth weights were similar, 28‐day‐old HP piglets were 18% heavier than NP piglets (P < 0.01). Carcass protein content was 22% higher in HP than in NP offspring (P < 0.01). Despite a HP intake, kidney weight and glomerular fibrosis were unaltered in HP piglets. Only a 20% increase in glomerular volume was noted in HP piglets (P < 0.05) and restricted to the inner cortical area nephrons (P = 0.03). Plasma urea/creatinine ratio and proteinuria were unchanged in HP piglets. In conclusion, neonatal HP feeding in IUGR piglets significantly enhanced neonatal growth and tissue protein deposition but mildly affected glomerular volume. It can be speculated that a sustained tissue protein anabolism in response to HP intake have limited single nephron glomerular hyperfiltration.

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Neonatal Refeeding Syndrome and Clinical Outcome in Extremely Low‐Birth‐Weight Babies: Secondary Cohort Analysis From the ProVIDe Trial

Cormack

Jiang

Harding

et al. 2020

J Parenter Enteral Nutr

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Background Refeeding syndrome (RS) following preterm birth has been linked to high intravenous (IV) protein intake in the presence of low electrolyte supply. In extremely low‐birth‐weight (ELBW) babies, we aimed to determine the incidence of RS and associations with birth characteristics and clinical outcomes. Method Prospective cohort study of ELBW ProVIDe Trial participants in 6 New Zealand neonatal intensive care units. RS was defined as serum phosphate < 1.4 mmol.L−1 and total calcium > 2.8 mmol.L−1. Relationships between RS and other factors were explored using 2‐sample tests and logistic regression adjusted for sex, gestation, and birth‐weight z‐score. Results Of 338 babies (mean [SD] birth‐weight, 780 (134) g, gestational age, 25.9 [1.7] weeks), 68 (20%) had RS. Mortality was greater in babies with RS (32% vs 11%; P < .0001). More small‐ than appropriate‐for‐gestational‐age babies developed RS (22% vs 8%; P = .001). Growth from birth to 36 weeks’ corrected age was not different between babies who did and did not have RS. In logistic regression, the odds of RS decreased by 70% for each 1 mmol per kg−1.d−1 IV phosphate intake (odds ratio [OR], 0.3; CI, 0.1–0.6; P = .002) and increased by 80% for each 1 g.kg−1.d−1 IV protein intake (OR, 1.8; CI, 1.3–2.7; P = .002). Conclusions Neonatal RS is common in this cohort of ELBW babies and is associated with increased morbidity and mortality. Optimizing phosphate and calcium intakes in IV nutrition solutions may reduce RS and its consequences.

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Fatal Effects of a Neonatal High-Protein Diet in Low-Birth-Weight Piglets Used as a Model of Intrauterine Growth Restriction

Cited by 35 publications

References 47 publications

A high-protein neonatal formula induces a temporary reduction of adiposity and changes later adipocyte physiology

A high-protein neonatal formula induces a temporary reduction of adiposity and changes later adipocyte physiology

Neonatal high protein intake enhances neonatal growth without significant adverse renal effects in spontaneous IUGR piglets

Neonatal Refeeding Syndrome and Clinical Outcome in Extremely Low‐Birth‐Weight Babies: Secondary Cohort Analysis From the ProVIDe Trial

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