Fate of [14C]photoheptachlor in rabbits

Abstract

Tissue distribution, elimination, and metabolism of intraperitoneally administered photoheptachlor was studied in male rabbits. Biological half-life of the compound at 0.8 mg/kg dose level was about 70 days. The radioactivity was excreted almost exclusively in urine. Analyses of the urine extracts revealed that all the radioactivity was in the form of four metabolites, none of which corresponded to the parent compound. Gas chromatography-mass spectrometry analysis of one of the metabolites indicates that this metabolite arises by oxidative dechlorination of photoheptachlor and is eliminated both in free and conjugated form. Tissue distribution indicated that maximal amounts of the radiocarbon were present in visceral fat and the residues declined with the passage of time.The observation that cyclodiene insecticides undergo isomerization (Rosen et al., 1966) has resulted in numerous reports which show that these photoconversions are fairly common and take place not only under laboratory conditions but also in simulated and natural environments

Metabolic fate of cis-photochlordane in the rat. 1. Excretion, tissue distribution, and preliminary characterization of metabolites

“…Operation temperatures were as follows: inlet 220 °C, column 190 °C, and detector 220 °C. Purified metabolites were tested for the presence or absence of hydroxyl groups by treatment with Tri-Sil Z (Pierce Chemical Co., Rockford, IL) as described previously (Feroz and Khan, 1979a).…”

“…to be produced. Khan, 1977b); photoheptachlor in rabbit (Feroz and Khan, 1979b)], information on cts-photochlordane, a possible terminal residue of chlordane, is totally lacking. This report describes the results of excretion, storage, and biotransformations of cis-photochlordane in the rat.…”

Opinion of the Scientific Panel on contaminants in the food chain [CONTAM] related heptachlor as an undesirable substance in animal feed

“…The metabolism of intraperitoneally administered 14 C-photoheptachlor was studied in male rabbits by Feroz and Khan (1979). These authors found that radioactivity was excreted almost exclusively in urine.…”

“…However, much higher values were reported for lactating dairy cows (6 to 8 weeks, Bruce et al, 1965;Cook and Wilson, 1971) for pigs (10 to 12 weeks, Raisbeck et al, 1986) and for non lactating cattle (>20 weeks, Raisbeck et al, 1986). Feroz and Khan (1979) reported a half-life value of about 10 weeks for photoheptachlor intraperitoneally administered to rabbits.…”

Metabolism of cis- [14C]chlordane and cis-[14C]photochlordane in bluegill fish

“…Residues of chlordanes and their bioalteration products have been found in fish (Miles and Harris, 1973), pelicans (Blus et al, 1977), and aquatic invertebrates (Bugg et al, 1967;Godsil and Johnson, 1968;Tanita et al, 1976). Chlordanes are refractive to bioalteration in rats (Barnett and Borough, 1974;Brimfield et al, 1978) but more so in freshwater goldfish (Feroz and Khan, 1979a) and cichlid (Feroz and Khan, 1979b). Since cyclodienes are extremely toxic to fish (Tarzwell, 1963;Podowski et al, 1979), the knowledge of their fate (persistence, metabolism, and disposition) and effects on various fish species has been needed for a long time.…”