Fate of Escherichia coli O26 in Corn Silage Experimentally Contaminated at Ensiling, at Silo Opening, or after Aerobic Exposure, and Protective Effect of Various Bacterial Inoculants

Dunière, Lysiane; Gleizal, A.; Chaucheyras‐Durand, Frédérique; Chevallier, Isabelle; Thevenot-Sergentet, Delphine

doi:10.1128/aem.06320-11

Cited by 18 publications

(36 citation statements)

References 61 publications

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“…; Duniére et al . ). Die‐off of undesirable microbial populations in ensiled materials results from the production of weak organic acids (OAs, including lactic, acetic and propionic) and concomitant decreases in pH (around pH 4·0).…”

Section: Introductionmentioning

confidence: 97%

Sensitivity ofMycobacterium aviumsubspparatuberculosis,Escherichia coliandSalmonella entericaserotype Typhimurium to low pH, high organic acids and ensiling

2013

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Aims To evaluate the persistence of Mycobacterium avium subsp paratuberculosis (Myco. paratuberculosis), Salmonella enterica serotype Typhimurium (Salm.Typhimurium) and a commensal Escherichia coli (E. coli) isolate under the low pH and high organic acid (OA) conditions of ensiling of forages. Methods and Results Decay rates and the time required to obtain a 90% reduction in cell concentration were calculated following (i) exposure to buffered OA (pH 4·0, 5·0, 6·0 or 7·0) (ii) exposure to silage exudates and (iii) survival through ensiling of forage materials. Salm. Typhimurium had higher decay rates in silage exudates (−0·5601 day−1) than did E. coli (−0·1265 day−1), but both exhibited lower decay rates in silage than in OA or silage exudates. Myco. paratuberculosis showed no decrease in silage and decay rates in silage exudates were significantly lower (2–12 times) than for the other two organisms. Conclusions Escherichia coli, Salm. Typhimurium and Myco. paratuberculosis exhibit marked differences in response to acidity. All three organisms show acid resistance, but Myco. paratuberculosis in particular, if present in manure and applied to forage grasses, may survive the low pH and high OA of the ensilaging process; silage may therefore be a potential route of infection if ingested by a susceptible animal. Significance and Impact of Study This information contributes to the understanding of potential risks associated with silage preservation and contamination of livestock feed with manure‐borne pathogens.

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Section: Introductionmentioning

confidence: 97%

Sensitivity ofMycobacterium aviumsubspparatuberculosis,Escherichia coliandSalmonella entericaserotype Typhimurium to low pH, high organic acids and ensiling

2013

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“…Thus introduced, the microbes multiply in the green fodder and in silages produced from it (Davies et al, 1996;Holley et al, 2006;You et al, 2006). Ruminants are recognized to be a major reservoir of Shiga toxinproducing E. coli (Duniere et al, 2011). The combination of proper ensiling techniques and the utilization of selected bacterial inoculants appears to represent a good strategy to guarantee nutritional qualities of cattle feed while at the same time limiting the entry of pathogenic bacteria e.g.…”

Section: Alfalfa Before Ensilingmentioning

confidence: 99%

“…The combination of proper ensiling techniques and the utilization of selected bacterial inoculants appears to represent a good strategy to guarantee nutritional qualities of cattle feed while at the same time limiting the entry of pathogenic bacteria e.g. E. coli into the epidemiological cycle to improve the microbial safety of the food chain (Duniere et al, 2011). The addition of three bacterial inoculants, Propionibacterium sp., L. buchneri, and Leuconostoc mesenteroides (10 6 CFU g -1 ), was evaluated by Duniere et al (2011) for their abilities to control E. coli pathogen in corn silages.…”

Section: Alfalfa Before Ensilingmentioning

confidence: 99%

“…The addition of three bacterial inoculants, Propionibacterium sp., L. buchneri, and Leuconostoc mesenteroides (10 6 CFU g -1 ), was evaluated by Duniere et al (2011) for their abilities to control E. coli pathogen in corn silages. During ensiling, bacterial treatments did not significantly affect E. coli O26 elimination compared with the negative control, except for the L. buchneri, which showed E. coli strain-dependent inhibition (Duniere et al, 2011).…”

Section: Alfalfa Before Ensilingmentioning

confidence: 99%

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Different aspects of Lactobacillus inoculants on the improvement of quality and safety of alfalfa silage

Zielińska

Fabiszewska

Stefańska

2015

Chilean J. Agric. Res.

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There is a significant range of bacterial inoculants for forage ensiling, but there is still a need for formulations to improve the safety of feed. The objective of the study was to evaluate the usefulness of three lactobacilli strains in alfalfa (Medicago sativa L.) The following bacterial inoculants were used: Lactocacillus plantarum K KKP 593p (LPK), L. plantarum C KKP 788p (LPC), L. buchneri KKP 907p (LB), and mix of all three strains (LPK+LPC+LB). The application of bacterial inoculants in alfalfa ensiling resulted in a reduction of the total number of molds, Clostridium perfringens and Listeria sp. (up to 5, 7, and 5 times respectively for LB inoculant in comparison to untreated silage). Total inhibition of Salmonella sp. and Escherichia coli growth was achieved in silages treated with all inoculants except for LPC. Aerobic stability in the control silage was the lowest (77 h) and doubled under the influence of bacterial inoculants. The most stable according to aerobic stability was silage treated with LB inoculant (175 h), where the highest concentrations of acetic acid (4.8 g kg -1 ), propionic acid (0.7 g kg -1 ) and 1,2-propanediol (526 mg kg -1 ) were reported. The study discussed that it is important to evaluate not only the effect of bacterial inoculants on physicochemical and microbiological silage properties, as the presence and expression of antibiotic resistance genes in lactic acid bacteria have been reported. The results of antimicrobial susceptibility testing of the strains showed that almost all minimum inhibitory concentrations values for eight antibiotics were equal to or below the corresponding breakpoints proposed by the European Food Safety Authority, Additives and Products or Substances used in Animal Feed Panel.

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“…In the work presented here, the butyric acid contents of five types of silages were all lower than 0.5 % during 170 days of storage, indicating good preservation. It has been reported that propionic acid is produced from lactic acid, and the presence of butyric acid in silage is a sign of fermentation by undesirable microorganisms, which should be avoided at all costs (34). Furthermore, the ratio of lactic to acetic acid and the ratio of lactic to total organic acids in all five silages decreased with the prolonged storage, and the three silages (negative control and those treated with low dose of formic or acetic acid) had higher ratio of lactic to acetic acid than those treated with high dose of formic and acetic acids.…”

Section: Dynamic Analysis Of the Fermentation Quality During 170 Daysmentioning

confidence: 99%

Effects of Formic Acid or Acetic Acid on the Mixed-Storage Quality of Air-Dried Maize Stover and Cabbage Wastes and Microbial Community Analysis

Ren

Wang

Fan

et al. 2018

Food Technol. Biotechnol.

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SUMMARYA mixture of air-dried corn stover and cabbage waste was ensiled to preserve lignocellulosic biomass for use as biofuel. Furthermore, the effects of different fresh mass fractions (0.3 and 0.6 %) of formic or acetic acid on the mixed silage quality were evaluated to guarantee its quality. The application of formic or acetic acid prior to mixing the silage led to higher water-soluble carbohydrate fractions than the negative control, indicating that both acids contributed to preservation of water-soluble carbohydrates during storage for 170 days. The dry matter content was also increased after storage from 90 to 170 days. It was found that the content of neutral and acid detergent fibre, cellulose and holocellulose (the sum of cellulose and hemicellulose) in mixed silage treated with formic or acetic acid was significantly lower than that obtained in the negative control. The pH and the ratio of ammoniacal nitrogen to total nitrogen in mixed silage treated with acetic acid also significantly decreased. Furthermore, the addition of formic or acetic acid significantly weakened the fermentation intensity of lactic acid, depending on the ratio of lactic to acetic acid, as well as the ratio of lactic acid to total organic acids. The number of bacterial species and their relative abundance shifted during silage mixing, wherein microbial communities at phylum level mainly consisted of Proteobacteria and Firmicutes. The dominant bacteria were also observed to shift from Lactobacillus and Enterobacter in presilage biomass to Lactobacillus and Paralactobacillus. Specifically, Enterobacter disappeared after 130 days of storage. In conclusion, the addition of a low dose of acetic acid to fresh mass (0.3 %) could effectively improve the fermentation quality and is conducive to the preservation of the organic components.

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Fate of Escherichia coli O26 in Corn Silage Experimentally Contaminated at Ensiling, at Silo Opening, or after Aerobic Exposure, and Protective Effect of Various Bacterial Inoculants

Cited by 18 publications

References 61 publications

Sensitivity ofMycobacterium aviumsubspparatuberculosis,Escherichia coliandSalmonella entericaserotype Typhimurium to low pH, high organic acids and ensiling

Sensitivity ofMycobacterium aviumsubspparatuberculosis,Escherichia coliandSalmonella entericaserotype Typhimurium to low pH, high organic acids and ensiling

Different aspects of Lactobacillus inoculants on the improvement of quality and safety of alfalfa silage

Effects of Formic Acid or Acetic Acid on the Mixed-Storage Quality of Air-Dried Maize Stover and Cabbage Wastes and Microbial Community Analysis

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