Fate of hamster oviductin in the oviduct and uterus during early gestation

Roux, Emmanuelle; Bleau, G.; Kan, Frederick W. K.

doi:10.1002/(sici)1098-2795(199703)46:3<306::aid-mrd9>3.0.co;2-t

Cited by 10 publications

(12 citation statements)

References 65 publications

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“…It remains unclear what function the very rapidly migrating proteins detected in the hamster may exert. However, recent studies suggested that oviductal fluid glycoprotein with high molecular weight, referred to as oviductin, can bind the endometrium and that it may have a function in the implantation of developing blastocysts in the hamster [17,18]. Further biophysical or biochemical investigations may ultimately allow us to discover the physiological importance of each protein component, thereby elucidating the suitable milieu for early development of hamster embryos.…”

Section: Discussionsupporting

confidence: 65%

Pregnancy- and Age-dependent Changes in Protein Profiles of Uterine Luminal Fluid in the Hamster.

Suzuki

Toyokawa

2000

Journal of Reproduction and Development

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Abstract. The protein compositions of uterine secretions in young and aged hamsters during early pregnancy were studied by polyacrylamide slab-gel electrophoresis. Uterine luminal fluids were collected by flushing with a small amount of saline. A broad area of very rapidly migrating proteins, which was undetectable in plasma samples, appeared in the uterine fluids on Days 4 and 5 of pregnancy, which is when implantation occurs in this species. More protein bands were noted in this region in aged females in contrast to those of young females. Further, in the uterine fluids of aged hamsters, a post-albumin protein band disappeared at estrus and on Day 1 and the protein compositions in the post-transferrin region differed between the young and aged females. The qualitative changes in the protein content of the uterine fluids may account for the high rate of embryonic loss in aged hamsters.

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Section: Discussionsupporting

confidence: 65%

Pregnancy- and Age-dependent Changes in Protein Profiles of Uterine Luminal Fluid in the Hamster.

Suzuki

Toyokawa

2000

Journal of Reproduction and Development

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“…The presence of oviductin on the surface of the uterine epithelium was attributed to the flow of oviduct fluid from the oviduct into the lumen of the uterus. A subsequent study on the fate of hamster oviductin carried out in our laboratory using the monoclonal antibody showed that, during early gestation, immunoreactivity to oviductin in the uterus diminished to an almost total disappearance at the time of implantation (Roux et al 1997). The current results suggest that oviductin is taken up by the uterine epithelium, although the reason for this is unknown and requires further investigation.…”

Section: Discussionmentioning

confidence: 99%

Detection of Nascent and/or Mature Forms of Oviductin in the Female Reproductive Tract and Post-ovulatory Oocytes by Use of a Polyclonal Antibody Against Recombinant Hamster Oviductin

McBride

Boisvert

Bleau

et al. 2004

J Histochem Cytochem.

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S U M M A R YOviductins belong to a family of glycoproteins that have been suggested to play several roles during the early processes of reproduction. Recently, a polyclonal antibody was raised against recombinant hamster oviductin (rhaOv m ). Here the anti-rhaOv m antibody was used to investigate the sites of localization of oviductin in the female golden hamster. In the hamster oviduct, immunolabeling was restricted to the content of the Golgi saccules and secretory granules of the non-ciliated oviduct cells. After its release into the lumen, oviductin becomes associated with the zona pellucida of post-ovulatory oocytes. In unfertilized oocytes, oviductin was also detected in membrane invaginations along the oolemma and in some vesicles within the ooplasm. Furthermore, oviductin was detected over the microvilli and within multivesicular bodies of uterine epithelial cells. Western blotting analysis revealed the presence of oviductin in the hamster oviduct but not in the uterus or ovary. In the oviduct, the anti-rhaOv m antibody detected a polydispersed band corresponding to native oviductin (160-350 kD) and several lower molecular weight bands ( Ͻ 100 kD) corresponding to nascent and partially glycosylated forms of oviductin. The anti-rhaOv m antibody provides an additional tool for investigation into the cytochemical and biochemical properties of different forms of hamster oviductin in the female reproductive tract.

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“…The endometrial epithelium is a continuum of the oviductal epithelium and shares the same embryonic origin, and it is not surprising that several genes including steroid receptors, transcription factors, and growth factors are expressed commonly by both the uterine and oviductal epithelium [31]. In this context, OVGP1 is considered as an exception as its transcripts and protein are not detected in the endometrium of most species, except the hamster [29,32,33]. In our attempts to identify embryo-regulated molecules, we serendipitously discovered the expression of OVGP1 in the mouse endometrial epithelium during embryo implantation.…”

Section: Introductionmentioning

confidence: 99%

Oviductal glycoprotein 1 (OVGP1) is expressed by endometrial epithelium that regulates receptivity and trophoblast adhesion

Laheri

Ashary

Bhatt

et al. 2018

J Assist Reprod Genet

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Fate of hamster oviductin in the oviduct and uterus during early gestation

Cited by 10 publications

References 65 publications

Pregnancy- and Age-dependent Changes in Protein Profiles of Uterine Luminal Fluid in the Hamster.

Pregnancy- and Age-dependent Changes in Protein Profiles of Uterine Luminal Fluid in the Hamster.

Detection of Nascent and/or Mature Forms of Oviductin in the Female Reproductive Tract and Post-ovulatory Oocytes by Use of a Polyclonal Antibody Against Recombinant Hamster Oviductin

Oviductal glycoprotein 1 (OVGP1) is expressed by endometrial epithelium that regulates receptivity and trophoblast adhesion

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