Fate of Listeria monocytogenes Inoculated onto the Surface of Model Turkey Frankfurter Pieces Treated with Zein Coatings Containing Nisin, Sodium Diacetate, and Sodium Lactate at 4°C

Abstract

The antimicrobial effects of zein coatings containing nisin, sodium lactate, and sodium diacetate against Listeria monocytogenes on turkey frankfurters at 4 degrees C were determined. Our objectives were to determine whether zein, nisin, lactate, and diacetate alone or in combination could control the growth of L. monocytogenes on full-fat turkey frankfurters at 4 degrees C and to determine whether lactate or diacetate had any synergistic effect on the activity of nisin. Turkey frankfurter pieces surface inoculated with L. monocytogenes strain V7 were treated with zein-ethanol-glycerol (ZEG), zein-propylene-glycol (ZPR), ethanol-glycerol (EG), propylene glycol (PR), nisin (N), sodium lactate (L), or sodium diacetate (D) alone or in combination. Over 28 days, treatment with N or D alone reduced L. monocytogenes counts on frankfurters by 6.6 or 6.3 log CFU/g, respectively. N-D treatment reduced L. monocytogenes by 6 log CFU/g. The zein solvents EG and PR reduced L. monocytogenes by about 5.6 and 5.2 log CFU/g, respectively, similar to the results obtained with ZEG and ZPR, which suggests that zein powder per se had no antimicrobial activity. After 28 days, ZEG-N-D, ZEG-N-D-L, ZPR-N-D, and ZPR-N-D-L yielded no detectable CFU. L alone was ineffective. No synergies were observed. N and D when used singly and the combinations of N-D, ZEG-N-D, ZEG-N-D-L, ZPR-N-D, ZPR-N-D-L, EG, and PR were effective as inhibitors of the growth of recontaminating L. monocytogenes cells on full-fat turkey frankfurters.

Synthesis, characterization and antibacterial activity of biodegradable films prepared from Schiff bases of zein

“…The obtained results revealed that the zein-based film have no antibacterial activity against these bacterial strains. These results are in agreement with previous works of Janes and his colleagues (2002) and Lungu and Johnson (2005), as they showed that zein films had no effect on suppressing the growth of L. monocytogenes. Whereas, Schiff bases films exhibited higher antibacterial effect on the tested strains comparing with zein film.…”

Effect of storage time on physicochemical and textural properties of sausages covered with oxidized banana starch film with and without betalains

“…Naturally sourced materials such as starch are an alternative for use as films or coverings for fruits (Pan, Chen, & Lai, 2013) and vegetables (Das, Dutta, & Mahanta, 2013). For meat products, several different biodegradable materials have been proposed as coatings including polysaccharides such as chitosan (Kanatt, Rao, Chawla, & Sharma, 2013), cellulose (Luchansky & Call, 2004), carrageenans (Martins et al, 2012), alginate (Juck, Neetoo, & Chen, 2010), pectins (Kang et al, 2007) and their various formulations (Bierhalz, Da Silva, & Kieckbusch, 2012) as well as proteins including gelatin, corn proteins and collagen (Lungu & Johnson, 2005). One potential source of food packaging and coatings that has been less studied is film from starches.…”

Effects of Lactococcus lactis on Composition of Intestinal Microbiota: Role of Nisin

“…The ability of either bacteriocins or bacteriocin-producing strains to restrict the growth of pathogenic bacteria such as Listeria monocytogenes in various food products is well described. The majority of the investigations are carried out on ready-to-eat foods including cheese (16) and meat products (17,19); however, fish (23) and vegetables (1) can also be preserved by bioprotection.…”