Fate of silver nanoparticles in wastewater and immunotoxic effects on rainbow trout

Bruneau, Audrey; Turcotte, Patrice; Pilote, Martin; Gagné, François; Gagnon, Christian

doi:10.1016/j.aquatox.2016.02.013

Cited by 75 publications

(38 citation statements)

References 54 publications

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“…Waterborne exposure was confirmed by measuring total silver in the water and in the gills, liver and spleen tissues. The controls and solvent controls showed only trace levels of silver in the water as expected, and in the gills a background similar to that reported in trout by Bruneau et al (2016). The AgNO 3 treatment achieved a measured concentration in the water that was very close to the nominal total concentration.…”

Section: Confirming Silver Exposuresupporting

confidence: 83%

“…However, there was a significant decrease in the phagocytic activity of the isolated macrophages in the Ag NP treatment compared to unexposed controls and a AgNO 3 treatment. There was also increased cyclo-oxygenase activity in the livers of the fish (Bruneau et al, 2016), that could be interpreted as early evidence of inflammatory pathway activation. Katuli et al (2014) also showed that acute exposure of zebrafish to 6.76 mg l −1 of Ag NPs for four days could cause biochemical disturbances to the blood including NaCl depletion, elevated cortisol and glucose, and inhibition of erythrocyte acetylcholinesterase activity.…”

Section: Introductionmentioning

confidence: 88%

“…A few in vivo studies are currently available that report some effects of Ag NPs on some parts of the haematopoietic system. Bruneau et al (2016) exposed rainbow trout to a 10% (v/v) dilution of wastewater effluent with a Ag NP mass concentration of 40 µg l −1 for 96 h and found no decrease in lymphocyte or macrophage viability extracted from the pronephros. However, there was a significant decrease in the phagocytic activity of the isolated macrophages in the Ag NP treatment compared to unexposed controls and a AgNO 3 treatment.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Low hazard of silver nanoparticles and silver nitrate to the haematopoietic system of rainbow trout

Clark

Shaw

Handy

2018

Ecotoxicology and Environmental Safety

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Silver nanoparticles (Ag NPs) are known for their antibacterial properties and are used in a growing number of nano-enabled products, with inevitable concerns for releases to the environment. Nanoparticles may also be antigenic and toxic to the haematopoietic system, but the immunotoxic effect of Ag NPs on non-target species such as fishes is poorly understood. This study aimed to assess the effect of Ag NP exposure via the water on the haematopoietic system of rainbow trout, Oncorhynchus mykiss, and to determine whether or not the hazard from Ag NPs was different from that of AgNO. Fish were exposed for 7 days to a control (dechlorinated Plymouth freshwater), dispersant control, 1µgl Ag as AgNO or 100µgl Ag NPs. Animals were sampled on days 0, 4 and 7 for haematology, tissue trace metal concentration, biochemistry for evidence of oxidative stress/inflammation in the spleen and histopathology of the blood cells and spleen. The Ag NP treatment significantly increased the haematocrit, but the haematological changes were within the normal physiological range of the animal. Thrombocytes in spleen prints at day 4, and melanomacrophage deposits at day 7 in the spleen, of Ag NP exposed-fish displayed significant increases compared to all the other treatments within the time point. A dialysis experiment confirmed that dissolution rates were very low and any pathology observed is likely from the NP form rather than dissolved metal released from it. Overall, the data showed subtle differences in the effects of Ag NPs compared to AgNO on the haematopoietic system. The lack of pathology in the circulating blood cells and melanomacrophage deposits in the spleen suggests a compensatory physiological effort by the spleen to maintain normal circulating haematology during Ag NP exposure.

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Section: Confirming Silver Exposuresupporting

confidence: 83%

Section: Introductionmentioning

confidence: 88%

Section: Introductionmentioning

confidence: 99%

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Low hazard of silver nanoparticles and silver nitrate to the haematopoietic system of rainbow trout

Clark

Shaw

Handy

2018

Ecotoxicology and Environmental Safety

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“…An in vivo sub-acute immunotoxicity study which was carried out with rainbow trout showed that approximately 12 nm of Ag NPs caused immunosuppression and inflammation-inducing effects on the fish after 96 hours [104]. In another in vivo study using 20-100 nm of Ag NPs, it was observed that Ag NPs almost completely suppressed natural killer (NK) cell activity and decreased the production of interferon-γ, interleukin (IL)-10 and IL-6 at the end of the 28 days in rats [105].…”

Section: In Vivo Toxicological Information and Experiments About Silvmentioning

confidence: 99%

Assessment of Nano-toxicity and Safety Profiles of Silver Nanoparticles

Budama‐Kilinc¹,

Çakır-Koç²,

Zorlu³

et al. 2018

Silver Nanoparticles - Fabrication, Characterization and Applications

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Nanotoxicology, which is related with toxic potentials of nanoparticles (NPs) and their adverse effects on living organisms and environment, is a sub-branch of toxicology discipline. Nano-toxicity of NPs depends on their doses, unique chemical, and physical properties. Nowadays, silver (Ag) NPs are used in many consumer and scientific applications such as antimicrobial and pharmaceutical applications, water purification systems, textile industry, and food packaging processes. However, the information that about their nano-toxic potentials is still not complete, and it is considered that several parameters of Ag NPs such as size, shape, surface, and stability affect the toxic potential in different ways. Nano-toxic potentials of Ag NPs were mentioned as in vivo, in vitro, and in silico the studies. In this chapter, it was evaluated the common unique properties of NPs are related with nanotoxicology such as size, surface area and modifications, shape, agglomeration status, and dose. : in vivo, in vitro, in silico, nanoparticles, nano-toxicity, silver within organism, and the mechanisms of the actions. At the same time, this concept involves the isolation of the poisons, the analysis of toxic materials as quantitative and qualitative besides that risk analyses, optimization processes, and treatments of poisons [1]. Keywords

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“…The effect of silver nanoparticles on the Japanese medaka [15], Zebrafish [16] and Rainbow trouts [17] proves the toxic effect of silver nanoparticles on these organisms. AgNPs causes damage to the nuclear material of the blood erythrocyte [18].…”

Section: Introductionmentioning

confidence: 99%

Ecotoxicology of Green Synthesized Silver Nanoparticles on Fresh Water Fish Mystus Gulio

Abirami

Jose

Govindarajulu

et al. 2017

Int J Pharm Pharm Sci

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Objective: Nanotechnology an advanced tool to synthesis atomic level particles. Increased application of silver nanoparticles results in the bioaccumulation of these particles in the environment. The biological effect of the green synthesized silver nanoparticles on the fresh water cat fish ws studied in the present study.Methods: Asystasia gangetica leaf extract was used to synthesize silver nanoparticles, the particles were characterized by scanning electron microscopy. Fifteen days after the introduction of fishes into the medium containing AgNPs, the fishes were sacrificed and the tissues were processed for biochemical and histological studies. Na + -K + Results:The results observed in the present study reveals a marked difference in the level of carbohydrate in the muscle tissue from 48.338±0.320 to 22.747±1.837 µg/100 mg wet tissue followed by gills and liver with 41.21% and 25.48% decrease. Total protein content was decreased from 649.094±1.429 to 491.56±0.925 µg/mg wet tissues. The drastic increase in the total lipids was observed in liver tissue of treated fishes from 0.240±0.004 to 0.408±0.005 mg/gm wet tissue. Na -ATPase was estimated using LeBel method. Estimation of carbohydrate was done by the Phenol-sulphuric acid method. Total protein was estimated using Lowry's method, Sulpho-Vanilline method was used to estimate the total lipids. + -K + Conclusion:The plant-mediated synthesized AgNPs shows a potential toxic effect on all the tissues studied; changes were observed in the normal architecture of tissues as well as in the biochemical parameters. To understand the mechanism of toxicity of these particles further studies at the molecular level has to be carried out.-ATPase activity of liver tissue increased from 0.153±0.001 to 0.225±0.003 in the liver tissue of treated fishes, followed by muscles and gills with 33.61% and 35% of the increase in the activity. Marked changes in the structure of gills with degenerated primary gill lamellae, necrosis, hyperplasia and fused primary lamellae were evident in experimental group. Necrosis, vacuolization, disintegrated nucleus and blood sinusoids were observed in liver tissues. Disintegration of myofibrils was evident in AgNP treated group of fishes.

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Fate of silver nanoparticles in wastewater and immunotoxic effects on rainbow trout

Cited by 75 publications

References 54 publications

Low hazard of silver nanoparticles and silver nitrate to the haematopoietic system of rainbow trout

Low hazard of silver nanoparticles and silver nitrate to the haematopoietic system of rainbow trout

Assessment of Nano-toxicity and Safety Profiles of Silver Nanoparticles

Ecotoxicology of Green Synthesized Silver Nanoparticles on Fresh Water Fish Mystus Gulio

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