Fatty acid analysis of four Azospirillum species reveals three groups

Schenk, Sigrid U.; Werner, Dietrich

doi:10.1007/bf00446764

Cited by 14 publications

(10 citation statements)

References 7 publications

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“…The five A. brasilense evaluated strains showed a similar pattern to that observed for several different strains of A. brasilense [14]. In general, the fatty acids pattern of this genus includes two major ones 18:1 cis-9 (52-63%) and 16:1 cis-9 (12-16.5%) and other seven fatty acids present in different amounts: 12:0, 14:0, 3-OH-14:0, 16:0, 18:0 and two unknown with 17 and 19 of ECL [14,53].…”

Section: Discussionsupporting

confidence: 73%

“…In general, the fatty acids pattern of this genus includes two major ones 18:1 cis ‐9 (52–63%) and 16:1 cis ‐9 (12–16.5%) and other seven fatty acids present in different amounts: 12:0, 14:0, 3‐OH‐14:0, 16:0, 18:0 and two unknown with 17 and 19 of ECL . Schenk and Werner showed that the only two unsaturated fatty acids accounted for approximately 80% of the total fatty acid content and, although 18:0 is in minor percentage of total fatty acid content, it characterizes A. brasilense . However, these authors did not discuss the differences between strains for several Azospirillum species.…”

Section: Discussionmentioning

confidence: 99%

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Physiological and biochemical characterization ofAzospirillum brasilensestrains commonly used as plant growth-promoting rhizobacteria

Salvo

Silva

Teixeira

et al. 2014

J. Basic Microbiol.

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Azospirillum is a plant growth-promoting rhizobacteria (PGPR) genus vastly studied and utilized as agriculture inoculants. Isolation of new strains under different environmental conditions allows the access to the genetic diversity and improves the success of inoculation procedures. Historically, the isolation of this genus has been performed by the use of some traditional culture media. In this work we characterized the physiology and biochemistry of five different A. brasilense strains, commonly used as cereal inoculants. The aim of this work is to contribute to pose into revision some concepts concerning the most used protocols to isolate and characterize this bacterium. We characterized their growth in different traditional and non-traditional culture media, evaluated some PGPR mechanisms and characterized their profiles of fatty acid methyl esters and carbon-source utilization. This work shows, for the first time, differences in both profiles, and ACC deaminase activity of A. brasilense strains. Also, we show unexpected results obtained in some of the evaluated culture media. Results obtained here and an exhaustive knowledge revision revealed that it is not appropriate to conclude about bacterial species without analyzing several strains. Also, it is necessary to continue developing studies and laboratory techniques to improve the isolation and characterization protocols.

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Section: Discussionsupporting

confidence: 73%

Section: Discussionmentioning

confidence: 99%

Physiological and biochemical characterization ofAzospirillum brasilensestrains commonly used as plant growth-promoting rhizobacteria

Salvo

Silva

Teixeira

et al. 2014

J. Basic Microbiol.

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“…Although FA profiles for different strains of Azospirillum have been reported (Schenk and Werner ), our work is the first to demonstrate that FA plays a role in the response to water deficit for this genus of bacteria and specifically for the A. brasilense Az39 strain. Mhamdi et al .…”

Section: Discussionmentioning

confidence: 58%

“…Similar to us, Boumahdi et al (2001) showed that small reductions in water activities of the growth media for Sinorhizobium melilotti and for Bradyrhizobia resulted in a gradual decrease in the degree of unsaturation. Although FA profiles for different strains of Azospirillum have been reported (Schenk and Werner 1988), our work is the first to demonstrate that FA plays a role in the response to water deficit for this genus of bacteria and specifically for the A. brasilense Az39 strain. Mhamdi et al (2014) showed that for Sinorhizobium strains, the main modification in response to osmotic stress (PEG) is preferential accumulation of the 19:0 cyclo and 18:0 SFA.…”

Section: Discussionmentioning

confidence: 65%

Reorganization of Azospirillum brasilense cell membrane is mediated by lipid composition adjustment to maintain optimal fluidity during water deficit

et al. 2015

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“…Sucrose utilization of Azospirillum amazonense was previously recognized as a main characteristic to separate it from Azospirillum lipoferum (Magalhues et al, 1983). All four characterized Azospirillum species were analysed in their fatty acid composition, revealing three groups ( Schenk and Werner, 1988).…”

Section: New Nz-fixing Speciesmentioning

confidence: 99%

Plant, Microbial and Soil Factors, Determining Nitrogen Fixation in the Rhizosphere

Werner

Berggold

Jaeger

et al. 1989

J Plant Nutrition & Soil

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A genotype effect on associative (rhizosphere) N2‐fixation was observed with two cultivars of Sorghum bicolor (nutans) with a maximum rate of 8 μmol C2H4 · h−1 · plant−1 in one genotype compared to 0.9 μmol in the other. Characteristics of the high fixing genotype were a reduced transpiration rate, a lower number of stomata and increased root exudate production per gram root dry weight with higher concentration of dicarboxylic acids. The bacterial rhizosphere composition revealed a three times higher number of N2‐fixing bacteria, a tenfold reduction of actinomycetes and a threefold reduction of Arthrobacter associated with the high fixing cultivar compared to the low fixing genotype. From these and other plant rhizospheres two new nitrogen fixing bacteria, Pseudomonas stutzeri and Erwinia herbicola, were characterized. With the N2‐fixing bacteria Azospirillum brasilense and Klebsiella pneumoniae an enhancement of specific nitrogenase activity by aromatic compounds, for example phenolics, the herbicide alachlor and the insecticide carbofuran was demonstrated. An oscillating nitrogenase activity in Azospirillum brasilense under microaerobic conditions was found, resulting from an encystation and deencystation under those conditions. Experiments with wheat roots demonstrated that reduced oxygen tensions, essential for a maximum rhizosphere N2‐fixation, reduced root growth significantly and altered the N‐metabolism of the roots.

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Fatty acid analysis of four Azospirillum species reveals three groups

Cited by 14 publications

References 7 publications

Physiological and biochemical characterization ofAzospirillum brasilensestrains commonly used as plant growth-promoting rhizobacteria

Physiological and biochemical characterization ofAzospirillum brasilensestrains commonly used as plant growth-promoting rhizobacteria

Reorganization of Azospirillum brasilense cell membrane is mediated by lipid composition adjustment to maintain optimal fluidity during water deficit

Plant, Microbial and Soil Factors, Determining Nitrogen Fixation in the Rhizosphere

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