Fatty acid and sterol composition of the antarctic amphipod Themisto gaudichaudii guerin 1828

Fricke, H.; Oehlenschläger, Jörg

doi:10.1016/0305-0491(88)90258-1

Cited by 9 publications

(2 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The main component contained in the sterol fraction was cholesterol and the minor constituents were cholesta‐5,22‐dien‐3β‐ol, cholesta‐5,24‐dien‐3β‐ol, 24‐methylenecholest‐5‐en‐3β‐ol, and 24‐ethylidenecholest‐5‐en‐3β‐ol. Fricke and Oehlenschlaeger 63 identified cholesterol, desmosterol, and 22‐dehydrocholesterol as the main sterol composition of the Antarctic amphipod, Themisto gaudichaudii .…”

Section: Composition Of Sterolsmentioning

confidence: 99%

Sterols in marine invertebrates

Kanazawa

2001

Fisheries Sci

124

View full text Add to dashboard Cite

The sterol composition of marine invertebrates, crustaceans, molluscs, echinoderms, coelenterates, and sponges has been re‐examined by using improved analytical techniques. As a result, the sterol composition of marine invertebrates has been shown to be more complex mixtures including many new types of sterols with unusual steroid nuclei or with non‐conventional side chains. Some crustaceans and molluscs are of importance as seafood and aquaculture species. Crustaceans and some molluscs require dietary sources of sterol for growth and survival because of the absence of de novo sterol‐synthesizing ability. The present paper gives a review of the requirement and nutritive value of sterols, biosynthesis and metabolism of sterols, and composition and structure of sterols in marine invertebrates.

show abstract

Section: Composition Of Sterolsmentioning

confidence: 99%

Sterols in marine invertebrates

Kanazawa

2001

Fisheries Sci

124

View full text Add to dashboard Cite

show abstract

“…The increased percent value of the neutral lipid in the embryo may be due to the presence of glycerol, free fatty acids (unpublished data), and different carotenoids (Kour and Subramonium, 1992). Cholesterol was found to be the dominant sterol in many crustaceans (Ikekawa, 1985;Fricke and Oehlenschlager, 1988). In E. asiatica, 4% of the neutral lipid fraction of the I stage embryo was composed of cholesterol ( Table 2), but the amount is slightly lower in the Lv I1 (3% of the neutral lipid fraction).…”

Section: Discussionmentioning

confidence: 99%

Purification and characterization of vitellogenin and lipovitellins of the sand crab Emerita asiatica: Molecular aspects of crab yolk proteins

Tirumalai

Subramoniam

1992

Molecular Reproduction Devel

View full text Add to dashboard Cite

In the mole crab Emerita asiatica, the main yolk proteins consist of two slow moving lipovitellins (Lv I and Lv II) of glycolipoprotein nature. Lv I cleaves into subunits (MW: 109,000 and 105,000) and Lv II gives rise to six subunits (MW: 65,000, 54,000, 50,000, 47,000, 44,000, and 42,000) in SDS-PAGE (with beta-mercaptoethanol). In order to observe the stability of Lv II as well as to achieve better resolution of the proteins, two different buffer systems (Phosphate buffered saline and tris-buffered saline), 40% sucrose, and glass distilled water were used as homogenizing media. Among them, better resolution was achieved with tris-buffered saline and 40% sucrose, and tris-buffered saline seems to be the ideal medium for elution of Lv II. The analysis of biochemical constituents of the major Lv II reveals a percentage composition of 69.325, 27.927, and 2.753 respectively for protein, lipid, and bound sugars. In the I stage embryo, protein comprises about 67.276%, lipid 29.65%, and bound sugars 3.015%. Vitellogenin (Vg) electrophoretically corresponding to the Lv I and Lv II was present in the female haemolymph during the entire period of embryogenesis. The number of subunits (8) of Vg in all stages remained unaltered and their approximate molecular weights were Vg1, 91,000; Vg2, 87,000; Vg3, 83,000; Vg4, 61,000; Vg5, 58,000; Vg6, 45,000; Vg7, 42,000; and Vg8, 38,000. Different proteins present in the embryos (I and IV stage) and the serum obtained from the animal carrying the I stage embryo were separated by gel-filtration in high performance liquid chromatography (HPLC). Sephadex (G-200) gel filtration chromatography was used to purify the Lv II in large quantity. Total lipid extracted from Lv II as well as the embryos belonging to different stages of development were separated into their constituent neutral, glycolipids, and phospholipids, using silicic acid column chromatography. Thin layer chromatography (TLC) was used to isolate the different phospholipids purified from various stages of embryos and Lv II. As many as seven different phospholipids were separated from Lv II and I and IX stage embryos; and whereas thin layer chromatogram of V and VI stage embryos showed six different phospholipids, embryos of VII and VIII stage contained four phospholipid species. Cholesterol, glycolipids, and individual phospholipids isolated from the Lv II and I stage embryo were quantified spectrophotometrically and the results were discussed.

show abstract

Lipids

Urich¹

1994

Comparative Animal Biochemistry

View full text Add to dashboard Cite

Fatty acid and sterol composition of the antarctic amphipod Themisto gaudichaudii guerin 1828

Cited by 9 publications

References 9 publications

Sterols in marine invertebrates

Sterols in marine invertebrates

Purification and characterization of vitellogenin and lipovitellins of the sand crab Emerita asiatica: Molecular aspects of crab yolk proteins

Lipids

Contact Info

Product

Resources

About