Fatty acid and triacylglycerol composition of seed and pericarp oils of the medicinal crop Withania somnifera (L.) Dunal cultivated in Sardinia (Italy)

Abstract

Plant cultivationW. somnifera wild germplasm was collected in Barbagia of Nuorese subregion (centraleastern Sardinia) in March 2016. In April 2016, the seeds, previously soaked in water, were placed in the cell seedling boxes and positioned in the Planta Medica Greenhouse, HBK (Hortus Botanicus Kalaritanum) Botanical Garden of Cagliari, with a controlled temperature (21-23°C), controlled humidity (about 85%) and natural light. After 15/20 days we detected 95% of the seeds germinated. In October 2016, the obtained seedlings were planted in the open field without the use of phytosanitary and fertilizers. Two rows with 20 individuals each were implanted with 140 cm row spacing and 100 cm plant spacing; irrigation as needed, only in summer. In March 2019, ripe berries (at optimum maturity stage) were collected from 10 individuals chosen from the 40 plants present in the field. The cultivation in the open field was possible because Sardinia winter is mild and not too rainy. MaterialsAll the chemicals used in this study were of analytical grade. Triolein (OOO), 1,2-dioleoyl-3linoleoy-rac-glycerol (OOL), 1,2-dilinoleoyl-3-oleoylrac-glycerol (LLO), trilinolein (LLL), tripalmitin (PPP), 1,2-dilinoleoyl-3-palmitoyl-rac-glycerol (LLP), trilinolenin (LnLnLn), standards of fatty acids (FA) (purity ≥98%), and all solvents used (purity ≥99.9%), were

Increased Stress Resistance and Lifespan in Chaenorhabditis elegans Wildtype and Knockout Mutants—Implications for Depression Treatment by Medicinal Herbs

“…Wighteone [ 61 ], sophoracoumestan A [ 62 ], and bavachinin [ 61 ] were found in Psoralea corylifolia . Linoleic acid [ 63 ] was identified in Withania somnifera .…”