Fatty acid binding protein 10 in the orange-spotted grouper (Epinephelus coioides): Characterization and regulation under pH and temperature stress

Qi, Zenghua; Liu, Yufeng; Wang, Weina; Yu, Xin; Xie, Fu-Xing; Wang, An‐Li

doi:10.1016/j.cbpc.2011.12.001

Cited by 6 publications

(4 citation statements)

References 54 publications

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“…In our previous studies with Japanese seabass (Lateolabrax japonicus), several fabp isoforms, i.e., fabp1, fabp2, fabp3, fabp4, and fabp7, have been cloned and characterized [10]. fabps from other fish species, such as zebrafish (Danio rerio) [11], orange-spotted grouper (Epinephelus coioides) [12], Japanese rice fish (Oryzias latipes; medaka) [7], three-spined stickleback (Gasterosteus aculeatus) [7], Atlantic salmon (Salmo salar) [8], goldfish (Carassius auratus) [13], golden pompano (Trachinotus ovatus) [14,15], and javelin goby (Synechogobius hasta) [16], have also been cloned and characterized.…”

Section: Introductionmentioning

confidence: 99%

Tissue Distribution and Nutritional Regulation of Fatty Acid-Binding Proteins (fabps) in Two Marine Teleosts, Turbot (Scophthalmus maximus), and Tiger Puffer (Takifugu rubripes)

Wang

Zhao

Liao

et al. 2022

Aquaculture Nutrition

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Fatty acid-binding proteins (fabps) play important roles in lipid homeostasis. In the present study, 7 fabp isoforms, namely, fabp1, fabp2, fabp3, fabp4, fabp6, fabp7, and fabp10, in two marine teleosts, were characterized. In general, turbot and tiger puffer fabp genes showed high identity to their orthologs in other fish species and mammals, but tiger puffer Fabp6 shared the lowest identity to its known orthologs in zebrafish and human. The tissue distribution patterns of fabps were generally in accordance with their function features. However, tiger puffer fabps, in particular Fabp1, Fabp2, Fabp6 and Fabp7, may have functions distinct from other teleosts, as indicated by the phylogenetic tree and tissue distribution patterns. In both species, high dietary lipid levels downregulated the expression of fabp2, fabp3, fabp6, and fabp7a but tended to upregulate the fabp1 expression. Starvation downregulated the expression of most fabps in both fish species, but the downregulation of fabp expression in turbot was much more drastic and earlier compared to tiger puffer. Long-term (30-day) starvation increased the fabp7 expression in tiger puffer and tended to increase the fabp6 expression in turbot. Results of this study contribute to fish fabp physiology and its nutritional regulation.

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Section: Introductionmentioning

confidence: 99%

Tissue Distribution and Nutritional Regulation of Fatty Acid-Binding Proteins (fabps) in Two Marine Teleosts, Turbot (Scophthalmus maximus), and Tiger Puffer (Takifugu rubripes)

Wang

Zhao

Liao

et al. 2022

Aquaculture Nutrition

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“…In similar experiments with the same general cold shock regime, Qi et al . identified elevated respiratory burst activity over 36 h post‐shock (2012), as well as elevated p53 protein expression and increased apoptotic total blood cell counts (2013). The upregulation of p53 and genes implicated in mitigating thermal and oxidative stress and cellular damage ( e.g ., CAT, HSP90, C3) was also observed in obscure puffers following cold stress (25 to 13°C, 1°C per h; Cheng et al ., 2017).…”

Section: Cold Stress and The Generalized Stress Response In Fishmentioning

confidence: 99%

An updated review of cold shock and cold stress in fish

Reid

Patrick²,

Rytwinski

et al. 2022

Journal of Fish Biology

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Temperature is critical in regulating virtually all biological functions in fish. Low temperature stress (cold shock/stress) is an often-overlooked challenge that many fish face as a result of both natural events and anthropogenic activities. In this study, we present an updated review of the cold shock literature based on a comprehensive literature search, following an initial review on the subject by M.R. Donaldson and colleagues, published in a 2008 volume of this journal. We focus on how knowledge on cold shock and fish has evolved over the past decade, describing advances in the understanding of the generalized stress response in fish under cold stress, what metrics may be used to quantify cold stress and what knowledge gaps remain to be addressed in future research. We also describe the relevance of cold shock as it pertains to environmental managers, policymakers and industry professionals, including practical applications of cold shock. Although substantial progress has been made in addressing some of the knowledge gaps identified a decade ago, other topics (e.g., population-level effects and interactions between primary, secondary and tertiary stress responses) have received little or no attention despite their significance to fish biology and thermal stress. Approaches using combinations of primary, secondary and tertiary stress responses are crucial as a research priority to better understand the mechanisms underlying cold shock responses, from short-term physiological changes to individual-and population-level effects, thereby providing researchers with better means of quantifying cold shock in laboratory and field settings.

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“…The molecular mechanism for FABP1's antioxidant function was reported to centre on the solvent accessible methionines (five out of seven) and single cysteine being responsible for scavenging ROS (7). Others have subsequently reported the antioxidant role of FABP in other species (8,9). Reports showed that when liver damage was induced by environmental stress on orange-spotted groupers, FABP10 (a paralog of FABP1) acts as an antioxidant (8).…”

mentioning

confidence: 99%

“…Reports showed that when liver damage was induced by environmental stress on orange-spotted groupers, FABP10 (a paralog of FABP1) acts as an antioxidant (8). Decreased expression of FABP1 in zebrafish larvae also was associated with lower superoxide dismutase activity and higher levels of malondialdehyde (MDA) (9). FABP1 T94A is a missense mutation in the human Fabp1 gene where the 94 th amino acid, a polar moderate sized threonine, is substituted for a non-polar, small sized alanine residue (11).…”

mentioning

confidence: 99%

The Antioxidant Activity of Recombinant Rat Hepatic Fatty Acid Binding Protein T94A Variant

Gong

Davies

et al. 2018

J Pharm Pharm Sci

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PURPOSE: Liver fatty acid binding protein (FABP1) is a cytoplasmic polypeptide that transports substrates throughout the cytosol and functions as an antioxidant. A common polymorphic variant, FABP1 T94A has a minor allele frequency of 26-38%, 8.3±1.9% homozygous in the human population. The purpose of this study was to mutate and isolate recombinant rat FABP1 to the T94A variant to evaluate the mutant’s antioxidant activity using in vitro studies. METHODS: Site-directed mutagenesis was used to generate a mutation in rat cDNA within a pGEX-6p-2 vector. This plasmid was transformed into competent cells and cultured for expression of FABP1 T94A mutant. The mutated protein was purified using GSTrap Fastflow columns within an ÄKTA FPLC system. A 2,7-dichlorofluorescein (DCF) assay was used to screen the T94A variant antioxidant activity. Additionally, Thiobarbituric Acid Reactive Substances (TBARS) assay was used in determining T94A mutant antioxidant activity in hydrophilic and lipophilic environments through the use of the azo compounds AAPH and MeO-AMVN, respectively and in the presence and absence of the long-chain fatty acid palmitate and α-bromo palmitate. RESULTS: Although the FABP1 T94A (20 μM) mutant significantly reduced DCF fluorescence compared to control (no protein; P< 0.001), there were no significant difference when compared to the wild-type (WT) FABP1. T94A was able to diminish the formation of malondialdehyde (MDA) in both lipophilic and hydrophilic systems. There were significant differences between T94A mutant and WT FABP1 at concentrations 1 and 10 μM (P< 0.05) in the hydrophilic milieu, however, this was not seen at 20 μM and also not seen in the lipophilic milieu at all concentrations. When T94A was pre-incubated with the long-chain fatty acids palmitate or α -bromo palmitate, MDA formation was decreased in both lipid peroxidation systems. There were no statistical differences between the WT FABP1 and T94A bound with fatty acids in both lipid peroxidation systems, however, there was a slight statistical difference when the T94A and WT FABP1 bound α-Br-PA in the AAPH lipid peroxidation system only. CONCLUSIONS: The T94A has antioxidant activity in both hydrophilic and lipophilic environments. The T94A variant of FABP1 does not have a loss of function in regard to acting as an antioxidant but the extent of function may be influenced by ligand binding. We conclude that populations having the minor T94A allele frequency would have similar ROS scavenging potential as those with nascent FABP1.

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Fatty acid binding protein 10 in the orange-spotted grouper (Epinephelus coioides): Characterization and regulation under pH and temperature stress

Cited by 6 publications

References 54 publications

Tissue Distribution and Nutritional Regulation of Fatty Acid-Binding Proteins (fabps) in Two Marine Teleosts, Turbot (Scophthalmus maximus), and Tiger Puffer (Takifugu rubripes)

Tissue Distribution and Nutritional Regulation of Fatty Acid-Binding Proteins (fabps) in Two Marine Teleosts, Turbot (Scophthalmus maximus), and Tiger Puffer (Takifugu rubripes)

An updated review of cold shock and cold stress in fish

The Antioxidant Activity of Recombinant Rat Hepatic Fatty Acid Binding Protein T94A Variant

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