Fatty acid binding proteins have the potential to channel dietary fatty acids into enterocyte nuclei

Esteves, Adriana; Knoll‐Gellida, Anja; Canclini, Lucía; Silvarrey, María Cecilia; André, Michèle; Babin, Patrick J.

doi:10.1194/jlr.m062232

Cited by 35 publications

(27 citation statements)

References 82 publications

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“…Interestingly, Mettl3 depletion not only prevented the decrease of L-Fabp and Fatp4 gene expression, but also upregulated the mRNA level of Abca1 and CD36, even without LPS stimulation. Thus, the previously reported important genes in fatty acid absorption, Fatp4 and CD36, were selected for further investigation (14,15,(37)(38)(39)(40). Similar upregulation of Fatp4 and CD36 protein expression was also observed using IB (Fig.…”

Section: Mettl3 Depletion Upregulates Expression Of Fatty Acid Transpmentioning

confidence: 54%

Mettl3 Deficiency Sustains Long-Chain Fatty Acid Absorption through Suppressing Traf6-Dependent Inflammation Response

Zong

Zhao

Wang

et al. 2019

The Journal of Immunology

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Section: Mettl3 Depletion Upregulates Expression Of Fatty Acid Transpmentioning

confidence: 54%

Mettl3 Deficiency Sustains Long-Chain Fatty Acid Absorption through Suppressing Traf6-Dependent Inflammation Response

Zong

Zhao

Wang

et al. 2019

The Journal of Immunology

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“…system (12)(13)(14)(15)(16)(17)(18)(19)(20)(21). Many publications using fluorescent lipid reagents did not examine the metabolism of these labels [with some asserting that some or all fluorescent FAs are not metabolizable (22)(23)(24)], but prior work has shown that 4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene (BODIPY® FL)-labeled FAs may be esterified and incorporated into products, including phospholipids, TGs, and CEs (15,(25)(26)(27)(28)(29).…”

mentioning

confidence: 99%

An HPLC-CAD/fluorescence lipidomics platform using fluorescent fatty acids as metabolic tracers

Quinlivan

Wilson

Růžička

et al. 2017

Journal of Lipid Research

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Fluorescent lipids are important tools for live imaging in cell culture and animal models, yet their metabolism has not been well-characterized. Here we describe a novel combined HPLC and LC-MS/MS method developed to characterize both total lipid profiles and the products of fluorescently labeled lipids. Using this approach, we found that lipids labeled with the fluorescent tags, 4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza--indacene (BODIPY FL), 4,4-difluoro-5-(2-thienyl)-4-bora-3a,4a-diaza--indacene [BODIPY(558/568)], and dipyrrometheneboron difluoride undecanoic acid (TopFluor) are all metabolized into varying arrays of polar and nonpolar fluorescent lipid products when they are fed to larval zebrafish. Quantitative metabolic labeling experiments performed in this system revealed significant effects of total dietary lipid composition on fluorescent lipid partitioning. We provide evidence that cholesterol metabolism in the intestine is important in determining the metabolic fates of dietary FAs. Using this method, we found that inhibitors of dietary cholesterol absorption and esterification both decreased incorporation of dietary fluorescent FAs into cholesterol esters (CEs), suggesting that CE synthesis in enterocytes is primarily responsive to the availability of dietary cholesterol. These results are the first to comprehensively characterize fluorescent FA metabolism and to demonstrate their utility as metabolic labeling reagents, effectively coupling quantitative biochemistry with live imaging studies.

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“…Fatty acid binding proteins (FABP) are involved in fatty acid uptake, transport, and metabolism (Glatz and van der Vusse 1996 ), serving as carriers of saturated and unsaturated long-chain fatty acids, eicosanoids, and other hydrophobic ligands to effector molecules in the cytosol and nucleus (Esteves et al 2015 ). In the present study, fabp2 gene expression was higher in ABT fed the test diets containing 15% lipid (15KO and 15KORO) than in the fish fed higher dietary lipid (20KO).…”

Section: Discussionmentioning

confidence: 99%

Performance, feed utilization, and hepatic metabolic response of weaned juvenile Atlantic bluefin tuna (Thunnus thynnus L.): effects of dietary lipid level and source

Betancor

Ortega

Gándara

et al. 2018

Fish Physiol Biochem

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Two trials were performed using extruded diets as on-growing feeds for weaned Atlantic bluefin tuna ( Thunnus thynnus ; ABT) to establish adequate dietary levels of both lipid and omega-3 long-chain polyunsaturated fatty acids (LC-PUFAs), and impacts on lipid metabolism via liver gene expression. In trial A, ABT were fed with either a commercial feed (Magokoro®; MGK) as a reference diet or two experimental feeds differing in lipid levels (15 or 20%) using krill oil (KO) as the single lipid source in order to estimate suitable lipid content. Fish fed MGK displayed the highest growth, followed by 15KO, and therefore a dietary lipid content of 15% was considered preferable to 20% at this stage. In trial B, fish were fed MGK, 15KO, or a feed containing 15% lipid with a blend of KO and rapeseed oil (RO) (1:1, v / v ; 15KORO). Fish fed 15KO and 15KORO showed no difference in weight gain, specific growth rate, and fork length. Increasing dietary lipid level or including vegetable oil, RO, in the feeds did not increase liver lipid content. Liver fatty acid compositions largely reflected dietary profiles confirming very limited endogenous LC-PUFA biosynthesis. Liver of ABT fed 15KO and 20KO displayed the highest contents of docosahexaenoic acid (DHA). The hepatic expression of genes encoding enzymes and transcription factors involved in lipid and fatty acid metabolism, as well as genes encoding antioxidant enzymes, showed that many of these genes were regulated by dietary lipid and LC-PUFA content. Results suggested that ABT juveniles can be on-grown on inert dry feeds that support good fish growth and the accumulation of DHA. Electronic supplementary material The online version of this article (10.1007/s10695-018-0587-9) contains supplementary material, which is available to authorized users.

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Fatty acid binding proteins have the potential to channel dietary fatty acids into enterocyte nuclei

Cited by 35 publications

References 82 publications

Mettl3 Deficiency Sustains Long-Chain Fatty Acid Absorption through Suppressing Traf6-Dependent Inflammation Response

Mettl3 Deficiency Sustains Long-Chain Fatty Acid Absorption through Suppressing Traf6-Dependent Inflammation Response

An HPLC-CAD/fluorescence lipidomics platform using fluorescent fatty acids as metabolic tracers

Performance, feed utilization, and hepatic metabolic response of weaned juvenile Atlantic bluefin tuna (Thunnus thynnus L.): effects of dietary lipid level and source

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