Fatty acid biosynthesis during the life cycle of Debaryomyces etchellsii

Arous, Fatma; Mechichi, Tahar; Aggelis, George

doi:10.1099/mic.0.000298

Cited by 12 publications

(4 citation statements)

References 43 publications

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“…Lipids of both Debaryomyces sp. tested strains presented lower quantities of C18:2 ( ω ‐6) as compared with the relevant studies in which D. etchellsii strain BM1 had been cultivated in shake‐flask nitrogen‐excess and nitrogen‐limited Gly‐based media, where the concentration of C18:2 ( ω ‐6) was always >29·2% w/w of total lipids (Arous et al ).…”

Section: Discussionmentioning

confidence: 79%

Isolation, identification and screening of yeasts towards their ability to assimilate biodiesel‐derived crude glycerol: microbial production of polyols, endopolysaccharides and lipid

et al. 2019

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Aims To assess the ability of various newly isolated or belonging in official collections yeast strains to convert biodiesel‐derived glycerol (Gly) into added‐value compounds. Methods and Results Ten newly isolated yeast strains belonging to Debaryomyces sp., Naganishia uzbekistanensis, Rhodotorula sp. and Yarrowia lipolytica, isolated from fishes, metabolized Gly under nitrogen limitation. The aim of the study was to identify potential newly isolated microbial candidates that could produce single‐cell oil (SCO), endopolysaccharides and polyols when these micro‐organisms were grown on biodiesel‐derived Gly. As controls producing SCO and endopolysaccharides were the strains Rhodotorula glutinis NRRL YB‐252 and Cryptococcus curvatus NRRL Y‐1511. At initial Gly (Gly0) ≈40 g l−1, most strains presented remarkable dry cell weight (DCW) production, whereas Y. lipolytica and Debaryomyces sp. produced non‐negligible quantities of mannitol and arabitol (Ara). Five strains were further cultivated at increasing Gly0 concentrations. Rhodotorula glutinis NRRL YB‐252 produced 7·2 g l−1 of lipid (lipid in DCW value ≈38% w/w), whereas Debaryomyces sp. FMCC Y69 in batch‐bioreactor experiment with Gly0 ≈80 g l−1, produced 30–33 g l−1 of DCW and ~30 g l−1 of Ara. At shake‐flasks with Gly0 ≈125 g l−1, Ara of ~48 g l−1 (conversion yield of polyol on Gly consumed ≈0·62 g g−1) was achieved. Cellular lipids of all yeasts contained in variable concentrations oleic, palmitic, stearic and linoleic acids. Conclusions Newly isolated, food‐derived and non‐previously studied yeast isolates converted biodiesel‐derived Gly into several added‐value metabolites. Significance and Impact of the Study Alternative ways of crude Gly valorization through yeast fermentations were provided and added‐value compounds were synthesized.

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Section: Discussionmentioning

confidence: 79%

Isolation, identification and screening of yeasts towards their ability to assimilate biodiesel‐derived crude glycerol: microbial production of polyols, endopolysaccharides and lipid

et al. 2019

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“…Subsequently, citric acid is converted to oxaloacetate and acetyl-CoA (the precursor of FA biosynthesis), through the action of ATP-dependent citrate lyase (ACL). It has been reported that a low or null activity of ICDH and a high activity of ACL in the cytoplasm are key factors for the accumulation of lipids [2,5,6,44,[119][120][121][122][123].…”

Section: Sugar Conversion Into Sco and Regulatory Mechanisms In Sugarmentioning

confidence: 99%

Lignocellulosic Biomass as a Substrate for Oleaginous Microorganisms: A Review

Valdés¹,

Mendonça²,

Aggelis³

2020

Preprint

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The microorganisms able of accumulating lipids in high percentages, known as oleaginous microorganisms, have been widely studied as an alternative for producing oleochemicals and biofuels. Microbial lipid, so called Single Cell Oil (SCO), production depends on several growth parameters, including the nature of the carbon substrate, which must be efficiently taken up and converted into storage lipid. Οn the other hand, substrates considered for large scale applications must be abundant and of low acquisition cost. Among others, lignocellulosic biomass is a promising renewable substrate containing high percentages of assimilable sugars (hexoses and pentoses). However, it is also highly recalcitrant and therefore it requires specific pretreatments in order to release its assimilable components. The main drawback of lignocellulose pretreatment is the generation of several by-products that can inhibit the microbial metabolism. In this review, we discuss the main aspects related to the cultivation of oleaginous microorganisms using lignocellulosic biomass as substrate, hoping to contribute to the development of a sustainable process for SCO production in the near future.

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“…Biochemical studies of various enzymes and genes related to lipid biosynthesis have been conducted to understand how oleaginous microbes accumulate lipids under conditions of nitrogen limitation. − Key enzymes in lipid biosynthesis, including AMP deaminase (AMPD), ATP-citrate lyase (ACL), malic enzymes (MEs), and acetyl-CoA carboxylase (ACC), cooperate to redirect carbon flux from central carbon metabolism to fatty acid synthesis. − Previous studies showed that nitrogen activates AMPD, an enzyme that breaks down cellular AMP, leading to the inhibition of isocitrate dehydrogenase (ICDH). − As a result, tricarboxylic acid cycle (TCA cycle) flux is blocked, and accumulated citrate is transported outside the mitochondria and cleaved into acetyl-CoA by ACL, redirecting intracellular carbon to fatty acid synthesis pathways . Therefore, AMPD is a crucial regulator for coping with nitrogen-limited conditions and inducing lipogenesis in oleaginous microbes.…”

Section: Introductionmentioning

confidence: 99%

“…18−20 As a result, tricarboxylic acid cycle (TCA cycle) flux is blocked, and accumulated citrate is transported outside the mitochondria and cleaved into acetyl-CoA by ACL, redirecting intracellular carbon to fatty acid synthesis pathways. 21 Therefore, AMPD is a crucial regulator for coping with nitrogen-limited conditions and inducing lipogenesis in oleaginous microbes. However, its function in the interaction of nitrogen metabolism and lipid synthesis in oleaginous fungi at the molecular level remains unclear.…”

Section: ■ Introductionmentioning

confidence: 99%

Role of AMP Deaminase in Mucor circinelloides: Implications for Nitrogen Utilization and Lipid Biosynthesis

Li,

Tahiri,

Yang

et al. 2023

J. Agric. Food Chem.

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Lipid accumulation in oleaginous organisms is initiated by AMP deaminase (AMPD) after nitrogen depletion because it mediates the concentration of intracellular adenosine monophosphate (AMP). However, the role of AMPD in lipogenesis in the oleaginous fungus Mucor circinelloide s is largely unknown. Therefore, we identified the genes (ampd1 and ampd2) encoding AMPD and investigated the role of AMPD in lipid synthesis in this fungus by overexpressing and deleting ampd genes. Deletion of ampd1 and ampd2 caused 21 and 28% increments in lipid contents under N-limited conditions, respectively. These increases were correlated with the activation of enzymes involved in lipogenesis and the alteration of energy balance. Unexpectedly, overexpression of ampd genes affected nitrogen consumption in both N-limited and N-excess media, which resulted in an increase in cell growth and lipid accumulation compared with the control strain when nitrogen was available. Furthermore, the increased lipid accumulation in the ampd-overexpressing mutants in N-excess media was accompanied by enhanced activities of lipid biosynthetic enzymes. These data suggested that nitrogen metabolism and energy metabolism are affected by AMPD, and overexpression of ampd genes induced lipid accumulation under nitrogen-rich conditions by mimicking the nitrogen limitation response. This highlights an intriguing function of AMPD in M. circinelloides.

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Fatty acid biosynthesis during the life cycle of Debaryomyces etchellsii

Cited by 12 publications

References 43 publications

Isolation, identification and screening of yeasts towards their ability to assimilate biodiesel‐derived crude glycerol: microbial production of polyols, endopolysaccharides and lipid

Isolation, identification and screening of yeasts towards their ability to assimilate biodiesel‐derived crude glycerol: microbial production of polyols, endopolysaccharides and lipid

Lignocellulosic Biomass as a Substrate for Oleaginous Microorganisms: A Review

Role of AMP Deaminase in Mucor circinelloides: Implications for Nitrogen Utilization and Lipid Biosynthesis

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