Fatty acid composition and lipid profiles as chemotaxonomic markers of phytopathogenic fungi Puccinia malvacearum and P. glechomatis

Wołczańska, Agata; Christie, William W.; Fuchs, Beate; Galuska, Christina E.; Kowalczyk, Bożena; Palusińska-Szysz, Marta

doi:10.1016/j.funbio.2021.05.008

Cited by 5 publications

(11 citation statements)

References 28 publications

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“…Glycerophospholipids are differentiated into several classes: PC and PE are the major ones and PI, PS, PG, and PA are the minor ones. 23 Consistent with previous reports, the PC and PE account for more than 50% of the total phospholipid species in P. sojae and so play major roles in the structure and function of different membranes, similar to the roles they play in other eukaryotes. 23 An increase in PC concentration has been demonstrated under stress conditions.…”

Section: Discussionsupporting

confidence: 90%

Untargeted lipidomics reveals lipid metabolism disorders induced by oxathiapiprolin in Phytophthora sojae

Liu

Chen

et al. 2023

Pest Management Science

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Background: Oxathiapiprolin, an oxysterol-binding protein inhibitor (OSBPI), shows unexceptionable inhibitory activity against plant pathogenic oomycetes. FRAC (Fungicide Resistance Action Committee) classifies it into the mode of action group F9 (lipid homeostasis and transfer/storage), but very little is known about the lipid metabolism of oomycete pathogens when subjected to oxathiapiprolin.Results: In this study, seven lipid categories and 1435 lipid molecules were identified in Phytophthora sojae, among which glycerolipids, glycerophospholipids, and sphingolipids account for 30.10%, 50.59%, and 7.28%, respectively. These lipids were categorized into 31 subclasses, which varied to different extents when treated with oxathiapiprolin. A total of 11 lipid subclasses showed significant changes. Among them, 10 lipid subclasses, lysophosphatidylcholine (LPC), lysophosphatidylethanolamine (LPE), phosphatidylcholine (PC), phosphatidylserine (PS), ceramide (Cer), triglyceride (TG), (o-acyl)-1-hydroxy fatty acid, diglycosylceramide, sphingoshine (So), and sitosterol ester, were significantly up-regulated, while digalactosyldiacylglycerol was the only lipid that was significantly down-regulated by a factor of almost three. These lipid molecules were further analyzed at the lipid species level. A total of 542 species were significantly altered when treated with oxathiapiprolin, including 212 glycerolipids [186 TG and 26 diglycerides (DG)], 167 glycerophospholipids (38 PC, 15 LPC, 19 LPE, seven PS, etc.), 156 sphingolipids (146 Cer, four So, etc.), and some other lipid molecules. Finally, from the orthogonal partial least-squares discrimination analysis model, variable importance for the projection score analysis showed that Cer, TG, and some glycerophospholipids contribute to the metabolic disorder when subjected to oxathiapiprolin. Conclusion: Glycerolipids, glycerophospholipids, and sphingolipids in P. sojae undergo significant changes with oxathiapiprolin treatment. These results provided valuable information for further understanding the function of the target protein and the mode of action of OSBPIs in oomycetes.

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Section: Discussionsupporting

confidence: 90%

Untargeted lipidomics reveals lipid metabolism disorders induced by oxathiapiprolin in Phytophthora sojae

Liu

Chen

et al. 2023

Pest Management Science

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“…The molecular profiles of PC like other phospholipids are limited by the genetic features that are characteristic for every species and genus and depend on the environmental conditions [9]. For PC of ascomycetous yeasts, particularly for well-documented Saccharomyces cerevisiae, 32:2 and 34:2 are the most abundant, followed by 32:1 and 34:1 [1,21,[31][32][33].…”

Section: Pc Molecular Species Diversitymentioning

confidence: 99%

“…Very little is still known about diversity of PC molecular species in basidiomycetes. In teliospores of two species from genus Puccinia (class Pucciniomycetes) 16:0/18:2 and/or 16:0/18:1 were the main molecules accompanied by 16:0/20:4, 16:1/20:4 and some other species bearing unsaturated and very long chain fatty acids [9]. In six very specific pathogenic species Malassezia (class Ustilagomycetes), which characterized by the absence of de novo synthesis of fatty acids, PC molecular species were distributed as follows 36:2 > 36:3 >> 36:4, 34:1, 34:2 [37].…”

Section: Pc Molecular Species Diversitymentioning

confidence: 99%

“…In basidial fungi, like in the majority of eukaryotic organisms, PC and PE are the most abundant phospholipid classes, representing 60-80% of the total GPL [7][8][9]. The relative content of PC and PE changes in ontogenesis, as well as under the influence of various biotic and abiotic factors.…”

Section: Introductionmentioning

confidence: 99%

“…As far as we are aware, only few reports on the PC structural diversity of basidial fungi have been presented to date. In phytopathogenic basidial fungi from genus Puccinia the major PC species were 34:2 (16:0/18:2 (in the author's edition, the data are presented as 16:0_18:2 and so on [9])) and 36:4 (16:0/20:4) for P. malvacearum and 32:0 (16:0/16:0), 34:1 (16:0/18:1), 34:2 (16:0/18:2), 36:2 (18:0/18:2) for P. glechomatis [9]. Such PA profiles with a high level of 34 carbon containing molecular species are quite typical for yeast, but seem to differ from filamentous basidiomycetes with a high proportion of C18:2, C18:1, the combination of which usually leads to the biosynthesis of C36:3 and C36:4 molecular species.…”

Section: Introductionmentioning

confidence: 99%

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Diversity of ESI-MS Based Phosphatidylcholine Profiles in Basidiomycetes

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Senik

Manzhieva

et al. 2022

JoF

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Phosphatidylcholines (PC) are the main membrane lipid constituents comprising more than 50% of total glycerophospholipids. They coordinate a number of cell functions, particularly cell growth, homeostasis, secretion, recognition and communication. In basidial fungi PC are synthesized via the Kennedy pathway as well as through methylation of phosphatidylethanolamines (PE) and then undergo remodeling in Lands cycle that replaces fatty acids in PC molecules. The molecular profile of PC is determined by the genetic features that are characteristic for every species and depend on the environment. Here we present the results of ESI-MS based analyses of PC profiles of 38 species of basidiomycetes belonging to Agaricales (12), Polyporales (17), Russulales (5), Gleophyllales (2), Cantharellales (1), Auriculariales (1), Phallales (1). Although the variety of PC molecular species of basidiomycetes is rather diverse (20–38 molecular species in every profile), only 1–3 main molecular species represent 70–90% of total PC content. The most abundant of them are C36:4 and C36:3, followed by C34:1, C34:2, C36:5, C36:2. In the majority of basidiomycetes, C36:4 reaches up to 50–70% of total PC molecular species. Based on the results of hierarchical cluster analysis four main types of PC profiles which characterized the studied fungi independently from their taxonomic position, ecology, trophic status, and hyphal differentiation have been revealed. Comparative analyses of studied fungi using PCA method have shown that species of Polyporales differ from those of Agaricales by higher variability of PC profiles.

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Global Analysis of 2-Hydroxy Fatty Acids by Gas Chromatography-Tandem Mass Spectrometry Reveals Species-Specific Enrichment in Echinoderms

Ding,

Chen,

et al. 2023

J. Agric. Food Chem.

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Abnormal levels of 2-hydroxy fatty acids (2-OH FAs) are characterized in multiple diseases, and their quantification in foodstuffs is critical to identify the sources of supplementation for potential treatment. However, due to the structural complexity and limited available standards, the comprehensive profiling of 2-OH FAs remains an ongoing challenge. Herein, an innovative approach based on gas chromatography-tandem mass spectrometry (GC-MS/MS) was developed to determine the full profile of these FA metabolites. MS and MS/MS spectra of the trimethylsilyl (TMS) derivatives of 2-OH fatty acid methyl esters (FAMEs) were collected for peak annotation by their signature fragmentation patterns. The structures were further confirmed by validated structure-dependent retention time (RT) prediction models, taking advantage of the correlation between the RT, carbon chain length, and double bond number from commercial standards and pseudostandards identified in the whole-brain samples from mice. An in-house database containing 50 saturated and monounsaturated 2-OH FAs was established, which is expandible when additional molecular species with different chain lengths and backbone structures are identified in the future. A quantitation method was then developed by scheduled multiple reaction monitoring (MRM) and applied to investigate the profiling of 2-OH FAs in echinoderms. Our results revealed that the levels of total 2-OH FAs in sea cucumber Apostichopus japonicas (8.40 ± 0.28 mg/g dry weight) and starfish Asterias amurensis (7.51 ± 0.18 mg/g dry weight) are much higher than that in sea urchin Mesocentrotus nudus (531 ± 108 μg/g dry weight). Moreover, 2-OH C24:1 is the predominant molecular species accounting for 67.9% of the total 2-OH FA in sea cucumber, while 2-OH C16:0 is the major molecular species in starfish. In conclusion, the current innovative GC-MS approach has successfully characterized distinct molecular species of 2-OH FAs that are highly present in sea cucumbers and starfish. Thus, these findings suggest the possibility of developing future feeding strategies for preventing and treating diseases associated with 2-OH FA deficiency.

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Fatty acid composition and lipid profiles as chemotaxonomic markers of phytopathogenic fungi Puccinia malvacearum and P. glechomatis

Cited by 5 publications

References 28 publications

Untargeted lipidomics reveals lipid metabolism disorders induced by oxathiapiprolin in Phytophthora sojae

Untargeted lipidomics reveals lipid metabolism disorders induced by oxathiapiprolin in Phytophthora sojae

Diversity of ESI-MS Based Phosphatidylcholine Profiles in Basidiomycetes

Global Analysis of 2-Hydroxy Fatty Acids by Gas Chromatography-Tandem Mass Spectrometry Reveals Species-Specific Enrichment in Echinoderms

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