Fatty acid composition of human adipose tissue from two anatomical sites in a biracial community

Kokatnur, M. G.; Oalmann, Margaret C.; Johnson, William D.; Malcom, Gray T.; Strong, Jack P.

doi:10.1093/ajcn/32.11.2198

Cited by 59 publications

(54 citation statements)

References 10 publications

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“…The majority of fatty acid content of adipose tissue is composed of oleic, palmitic, and linoleic acids (Kokatnur et al 1979), similar to olive oil. To determine whether the FP assay can distinguish between specific and nonspecific binding, GE3082 association with olive oil was measured.…”

Section: Development Of An Fp-based Binding Assaymentioning

confidence: 99%

Identification of the Protein Target of Myelin-Binding Ligands by Immunohistochemistry and Biochemical Analyses

Bajaj

LaPlante

Cotero

et al. 2012

J Histochem Cytochem.

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The ability to visualize myelin is important in the diagnosis of demyelinating disorders and the detection of myelin-containing nerves during surgery. The development of myelin-selective imaging agents requires that a defined target for these agents be identified and that a robust assay against the target be developed to allow for assessment of structure-activity relationships. We describe an immunohistochemical analysis and a fluorescence polarization binding assay using purified myelin basic protein (MBP) that provides quantitative evidence that MBP is the molecular binding partner of previously described myelin-selective fluorescent dyes such as BMB, GE3082, and GE3111.

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Section: Development Of An Fp-based Binding Assaymentioning

confidence: 99%

Identification of the Protein Target of Myelin-Binding Ligands by Immunohistochemistry and Biochemical Analyses

Bajaj

LaPlante

Cotero

et al. 2012

J Histochem Cytochem.

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“…Linoleic acid (18:2n-6) is the most common polyunsaturated fatty acid (PUFA) in human diets and mammalian tissue and is considered essential because humans lack delta-12 and delta-15 desaturase enzymes (9,15,29,33). Ingested linoleic acid is converted to longer and more unsaturated fatty acids through enzymatic desaturation and elongation in the endoplasmic reticulum of cells.…”

mentioning

confidence: 99%

“…Ingested linoleic acid is converted to longer and more unsaturated fatty acids through enzymatic desaturation and elongation in the endoplasmic reticulum of cells. Linoleic acid is the direct precursor to oxidized linoleic acid metabolites (OXLAMs) including 13-and 9-hydroxy-octadecadienoic acid (13-HODE ϩ 9-HODE) (9,15,29,33) (see http://www.genome.jp/kegg-bin/show_ pathway?map00591 for more detail). 13-HODE ϩ 9-HODE are stable oxidation products and have been linked to pathological conditions including atherosclerosis, diabetes, Alzheimer's disease, non-alcoholic steatohepatitis, psoriasis, chronic inflammation, obesity, and cancer (14,23,29,37).…”

mentioning

confidence: 99%

Metabolomics approach to assessing plasma 13- and 9-hydroxy-octadecadienoic acid and linoleic acid metabolite responses to 75-km cycling

Nieman

Shanely

Luo

et al. 2014

American Journal of Physiology-Regulatory, Integrative and Comp

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Nieman DC, Shanely RA, Luo B, Meaney MP, Dew DA, Pappan KL. Metabolomics approach to assessing plasma 13-and 9-hydroxy-octadecadienoic acid and linoleic acid metabolite responses to 75-km cycling. Am J Physiol Regul Integr Comp Physiol 307: R68 -R74, 2014. First published April 23, 2014 doi:10.1152/ajpregu.00092.2014.-Bioactive oxidized linoleic acid metabolites (OXLAMs) include 13-and 9-hydroxy-octadecadienoic acid (13-HODE ϩ 9-HODE) and have been linked to oxidative stress, inflammation, and numerous pathological and physiological states. The purpose of this study was to measure changes in plasma 13-HODE ϩ 9-HODE following a 75-km cycling bout and identify potential linkages to linoleate metabolism and established biomarkers of oxidative stress (F2-isoprostanes) and inflammation (cytokines) using a metabolomics approach. Trained male cyclists (N ϭ 19, age 38.0 Ϯ 1.6 yr, wattsmax 304 Ϯ 10.5) engaged in a 75-km cycling time trial on their own bicycles using electromagnetically braked cycling ergometers (2.71 Ϯ 0.07 h). Blood samples were collected preexercise, immediately post-, 1.5 h post-, and 21 h postexercise, and analyzed for plasma cytokines (IL-6, IL-8, IL-10, tumor necrosis factor-␣, monocyte chemoattractant protein-1, granulocyte colonystimulating factor), F2-isoprostanes, and shifts in metabolites using global metabolomics procedures with gas chromatography mass spectrometry (GC-MS) and liquid chromatography mass spectrometry (LC-MS). 13-HODE ϩ 9-HODE increased 3.1-fold and 1.7-fold immediately post-and 1.5 h postexercise (both P Ͻ 0.001) and returned to preexercise levels by 21-h postexercise. Post-75-km cycling plasma levels of 13-HODE ϩ 9-HODE were not significantly correlated with increases in plasma cytokines but were positively correlated with postexercise F 2-isoprostanes (r ϭ 0.75, P Ͻ 0.001), linoleate (r ϭ 0.54, P ϭ 0.016), arachidate (r ϭ 0.77, P Ͻ 0.001), 12,13-dihydroxy-9Z-octadecenoate (12,13-DiHOME) (r ϭ 0.60, P ϭ 0.006), dihomo-linolenate (r ϭ 0.57, P ϭ 0.011), and adrenate (r ϭ 0.56, P ϭ 0.013). These findings indicate that prolonged and intensive exercise caused a transient, 3.1-fold increase in the stable linoleic acid oxidation product 13-HODE ϩ 9-HODE and was related to increases in F 2-isoprostanes, linoleate, and fatty acids in the linoleate conversion pathway. These data support the use of 13-HODE ϩ 9-HODE as an oxidative stress biomarker in acute exercise investigations.

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“…Oleic acid (C18:1 cis-9) is a mono-unsaturated omega-9 fatty acid and is the most abundant fatty acid in human adipose tissue (Kokatnur et al, 1979).…”

Section: Ii214 Oleic Acid Preparationmentioning

confidence: 99%

“…To get an impression how PI3K in general might be involved in the development of NASH in vitro experiments were done, where HSC were stimulated with an unsaturated free fatty acid (oleic acid). Oleic acid (C18:1 cis-9) is a monounsaturated omega-9 fatty acid and is the most abundant fatty acid in human adipose tissue (Kokatnur et al, 1979). As NASH is characterized also by more FFA distribution from the adipose tissue to the liver (Donnelly et al, 2005) stimulation with oleic acid was thought to be an adequate way to simulate NASH in vitro.…”

Section: Iii71 Pi3k P110γ γ γ γ Expression During Activation Of Hscmentioning

confidence: 99%

The Role of PI3K p110gamma in chronic liver injury

Dostert¹,

Saugspier²,

Dorn³

et al. 2012

Z Gastroenterol

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Fatty acid composition of human adipose tissue from two anatomical sites in a biracial community

Cited by 59 publications

References 10 publications

Identification of the Protein Target of Myelin-Binding Ligands by Immunohistochemistry and Biochemical Analyses

Identification of the Protein Target of Myelin-Binding Ligands by Immunohistochemistry and Biochemical Analyses

Metabolomics approach to assessing plasma 13- and 9-hydroxy-octadecadienoic acid and linoleic acid metabolite responses to 75-km cycling

The Role of PI3K p110gamma in chronic liver injury

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