Fatty acid composition of lipids in day 7–13 blastocysts, serum and uterine fluid of rabbits

Tsujii, Hirotada; Matsuoka, Y; Obata, Ryuichiro; Hossain, Mahboob; Takagi, Yuji

doi:10.1007/s12522-009-0020-2

Cited by 6 publications

(6 citation statements)

References 31 publications

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“…PUFA in general, and linolenic acid in particular, support essential development processes in mammalian embryos [ 26 ]. Linoleic acid acts as a precursor for eicosanoids and regulates the processes of endocytosis or exocytosis, ion channel modulation, DNA polymerase inhibition and gene expression [ 27 ]. Moreover, linoleic acid stimulates protein kinase C, which is necessary for cell differentiation and growth [ 28 , 29 ].…”

Section: Discussionmentioning

confidence: 99%

“…Early morulae and compacted morulae can be found at 72 hpm. Palmitic acid, as the main SFA, was identified in abundant concentrations in more developed embryos [ 27 ] and is reported to be essential for FA elongation and desaturation in embryo development [ 34 ]. Moreover, incubation with palmitic acid showed that embryos can oxidize palmitic acid even at the single-cell stage, with subsequent increases, particularly from four-cell embryos onwards [ 20 ].…”

Section: Discussionmentioning

confidence: 99%

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Fatty Acid Profile of Blood Plasma at Mating and Early Gestation in Rabbit

Hadjadj

Hankele

Armero

et al. 2021

Animals

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The aim of this study was to analyse the fatty acid (FA) profile of blood plasma at mating and 72 hpm by gas chromatography. Moreover, the correlation between FA and ovulation rate, normal embryos and compacted morulae was estimated. Palmitic, linoleic, oleic and stearic were the highest FA concentrations at mating and 72 hpm. Most long chain saturated and PUFA were higher at 72 hpm than at mating, while MUFA were higher at mating. SFA, MUFA and PUFA were high and positively correlated. Correlation was 0.643 between MUFA at mating and ovulation rate, and 0.781 between MUFA and normal embryos, respectively. Compacted morulae were slightly correlated with SFA at mating (0.465). In conclusion, the FA profile of plasma varies depending on the reproductive cycle of the rabbit female, adapting to energetic requirements at mating and early gestation. Moreover, positive correlations are found between fatty acids and ovulation rate and embryo development and quality.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Fatty Acid Profile of Blood Plasma at Mating and Early Gestation in Rabbit

Hadjadj

Hankele

Armero

et al. 2021

Animals

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“…In embryos, similar protection mechanisms might exist; Tsujii et al. () found higher concentrations of saturated fatty acids than unsaturated fatty acids in rabbit embryos.…”

Section: The Influence Of Fat Feeding On the Oocytes And Embryonic LImentioning

confidence: 94%

“…Also, Tsujii et al. () emphasized that serum and oviduct fluids play an important role in the development of blastocysts. The concentrations of nutrients in tubal fluid are documented to be below their plasma concentrations (Leese and Barton ), which suggests that their overall transport across the tube occurs principally by diffusion (Leese and Gray ).…”

Section: Fat Feeding and The Effects On The Oocyte And Embryo Microenmentioning

confidence: 99%

Dietary Fat Supplementation and the Consequences for Oocyte and Embryo Quality: Hype or Significant Benefit for Dairy Cow Reproduction?

Leroy

Sturmey

Hoeck

et al. 2014

Reprod Domestic Animals

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In many countries, fat supplementation in the diet has become common in the dairy industry. There are several ideas as to how dietary fat could influence reproductive performance. Saturated fatty acids, such as palm oil, can increase milk yield but may aggravate negative energy balance and thus may impair fertility when fed during the first week post-partum. However, priming the lipid oxidation in the liver by feeding saturated fats during the dry period has recently been shown to be a potentially promising strategy to mitigate fat mobilization and liver accumulation post-partum. Furthermore, polyunsaturated fats (omega-3 fatty acids and conjugated linoleic acids) are fed to reduce the 'de novo' fat synthesis in the udder and thus the milk fat content, which may be of modest benefit for overall energy balance. Furthermore, omega-6 and omega-3 polyunsaturated fatty acids are reported to alter follicular growth, steroid synthesis and prostaglandin metabolism in the ovary and endometrium, respectively. Omega-6 fatty acids are believed to have pro-inflammatory and thus PGF2α-stimulating properties rendering them extra value as 'nutraceutical' early post-partum, while omega-3 fatty acids can weaken this inflammatory potency, leading to a higher chance of survival of the embryo when supplemented during the periconceptual period. Unfortunately, research results rarely provide a consensus in this perspective. The consequences of these fat-feeding strategies on oocyte and embryo quality remain an intriguing issue for debate. Fat feeding may alter the microenvironment of the growing and maturing oocyte of the early and older embryo and thus may affect reproductive outcome. We recently reported that dietary-induced hyperlipidaemic conditions can be harmful for embryo development and metabolism. However, to date, research results remain somewhat conflicting most probably due to differences in fat sources used, in diet and duration of supplementation and in experimental set-up in general.

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“…We then demonstrated that the lipid-rich inter-muscular environment allows fabT mutant growth in a FASII-dependent manner, while inhibiting the WT strain. While the overall muscle and uterine fluids environments where GAS multiply are very different, it is notable that their lipid concentrations, differ by ~1000-fold (150 mg/g muscle, compared to 20-200 µg/ml in uterine fluid 29,30 ). We suggest that exposure to high inter-muscular lipid availability would contribute to WT mortality.…”

Section: Discussionmentioning

confidence: 99%

The double-edged role of FASII regulator FabT inStreptococcus pyogenesinfection

Lambert,

Gaillard,

Wongdontree

et al. 2023

Preprint

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In Streptococcus pyogenes, the fatty acid (FA) synthesis pathway FASII is feedback-controlled by the FabT repressor bound to an acyl-Acyl carrier protein. FabT defects are associated with attenuated virulence in animal models. Nevertheless, fabT point mutations arise in vivo. To resolve this paradox, we identified conditions and biotopes in which fabT behavior is defective, as being human tissues, cells, or cell filtrates. We then defined biotopes in which fabT mutants have a growth advantage, as being lipid rich. In a proof of concept we demonstrate that fabT mutants emerge in this context, due to FASII derepression, which prevents environmental FA incorporation. Energy dissipation is identified as the principal defect of fabT mutants, such that specific nutrients are consumed but do not promote growth. Our findings elucidate the nature of the link between FabT and virulence, provide a rationale for fabT mutant emergence, and identify the defect that causes attenuated fabT infection.

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Fatty acid composition of lipids in day 7–13 blastocysts, serum and uterine fluid of rabbits

Abstract: Palmitic acid is the most abundant fatty acid in the blastocysts, serum and uterine fluids of rabbit during days 7-13 of pregnancy.

Cited by 6 publications

References 31 publications

Fatty Acid Profile of Blood Plasma at Mating and Early Gestation in Rabbit

Fatty Acid Profile of Blood Plasma at Mating and Early Gestation in Rabbit

Dietary Fat Supplementation and the Consequences for Oocyte and Embryo Quality: Hype or Significant Benefit for Dairy Cow Reproduction?

The double-edged role of FASII regulator FabT inStreptococcus pyogenesinfection

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