Fatty acid composition of phospholipids and neutral lipids and lipid peroxidation in human breast cancer and lipoma tissue

Hietanen, Eino; Punnonen, Kari; Punnonen, R.; Auvinen, O

doi:10.1093/carcin/7.12.1965

Cited by 48 publications

(28 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The stimulatory effect of high levels of dietary fat appears to act primarily at the promotional stage. In addition, fatty acids may affect initiation and promotion of breast cancer, perhaps related to the formation of reactive metabolites of unsaturated fatty acids (malondialdehyde) [Hietanen et al, 1986]. A current view is that the mammary tumorigenic-enhancing activities of a high-fat diet is, at least in part, through a caloric mechanism [Welsch, 1994].…”

Section: A Role For Dietary Fat In the Epidemiology Of Breast Cancermentioning

confidence: 99%

p53 As a mutagen test in breast cancer

Hill

Sommer

2002

Environ and Mol Mutagen

View full text Add to dashboard Cite

The p53 gene is mutated in about half of all tumors. The p53 gene can be used as a “mutagen test,” that is, the relative frequencies of the different types of mutation can be used as an epidemiological tool to explore the contribution of exogenous mutagens vs. endogenous processes in particular cancers. p53 has been used as a mutagen test in breast cancer. Surprisingly, the pattern of p53 mutations differs among 15 geographically and ethnically diverse populations. In contrast, mutation patterns in the human factor IX gene are similar in geographically and ethnically diverse populations. Diverse p53 mutation patterns in breast cancer are consistent with a significant contribution by a diversity of exogenous mutagens. Breast tissue may be uniquely sensitive to lipophilic mutagens because of its unique architecture, characterized by tiny islands of cancer‐prone mammary epithelial cells surrounded by a sea of adipocytes. Mammary epithelial cells may be differentially susceptible to released lipophilic mutagens preferentially concentrated in adjacent adipocytes and originating in the diet. To test this hypothesis, we developed a method for measuring mutation load from ethanol‐fixed, paraffin‐embedded human tissues immunohistochemically stained with anti‐p53 antibodies. Single cells staining positively for p53 overabundance are microdissected and the gene is sequenced. It is possible to identify individuals with a high mutation load in normal breast tissue and who are presumably at increased risk for breast cancer. In addition, analysis of the p53 gene with appropriate mutation detection methodology markedly improves the prediction of early recurrence, treatment failure, and death in breast cancer patients. Mutagen tests and mutation load measurements are useful tools to identify the role of mutagens in breast cancer. Environ. Mol. Mutagen. 39:216–227, 2002. © 2002 Wiley‐Liss, Inc.

show abstract

Section: A Role For Dietary Fat In the Epidemiology Of Breast Cancermentioning

confidence: 99%

p53 As a mutagen test in breast cancer

Hill

Sommer

2002

Environ and Mol Mutagen

View full text Add to dashboard Cite

show abstract

“…The degree of lipid unsaturation within the IMCL and EMCL pools is also of substantial clinical importance (1,12). Fatty acids are derived from dietary sources and intracellular biosynthesis, with lipids being composed of three fatty acid chains and a glycerol molecule.…”

mentioning

confidence: 99%

Distinct patterns of fat metabolism in skeletal muscle of normal-weight, overweight, and obese humans

Velan¹,

Said²,

Durst³

et al. 2008

American Journal of Physiology-Regulatory, Integrative and Comp

View full text Add to dashboard Cite

Velan SS, Said N, Durst C, Frisbee S, Frisbee J, Raylman RR, Thomas MA, Rajendran VM, Spencer RG, Alway SE. Distinct patterns of fat metabolism in skeletal muscle of normal-weight, overweight, and obese humans. Am J Physiol Regul Integr Comp Physiol 295: R1060 -R1065, 2008. First published July 30, 2008 doi:10.1152/ajpregu.90367.2008The link between body weight, lipid metabolism, and health risks is poorly understood and difficult to study. Magnetic resonance spectroscopy (MRS) permits noninvasive investigation of lipid metabolism. We extended existing two-dimensional MRS techniques to permit quantification of intra-and extramyocellular lipid (IMCL and EMCL, respectively) compartments and their degree of unsaturation in human subjects and correlated these results with body mass index (BMI). Using muscle creatine for normalization, we observed a statistically significant (P Ͻ 0.01) increase in the IMCL-to-creatine ratio with BMI (n ϭ 8 subjects per group): 5.9 Ϯ 1.7 at BMI Ͻ 25, 10.9 Ϯ 1.82 at 25 Ͻ BMI Ͻ 30, and 13.1 Ϯ 0.87 at BMI Ͼ 30. Similarly, the degree of IMCL unsaturation decreased significantly (P Ͻ 0.01) with BMI: 1.51 Ϯ 0.08 at BMI Ͻ 25, 1.30 Ϯ 0.11 at 25 Ͻ BMI Ͻ 30, and 0.90 Ϯ 0.14 at BMI Ͼ 30. We conclude that important aspects of lipid metabolism can be evaluated by two-dimensional MRS and propose that degree of unsaturation measured noninvasively may serve as a biomarker for lipid metabolic defects associated with obesity. magnetic resonance spectroscopy; lipid unsaturation; intramyocellular lipid; extramyocellular lipid THE AMOUNT OF BODY FAT is a risk factor for several obesityrelated disorders. Obesity is a known risk factor for the development of insulin resistance and diabetes and is a key component of metabolic syndrome. The causal relationship between increased dyslipidemia and adiposity and impaired glucose homeostasis is unclear, although it is known that lipid oversupply to the organs primarily involved in glucose homeostasis, that is, muscle, liver, and pancreas, leads to impaired insulin function in those tissues (22).Previous studies showed that intramyocellular lipid (IMCL) is increased with obesity and in non-insulin-dependent diabetes mellitus (9,11,15). It has been suggested that increased visceral adiposity and reduced lipid oxidation might contribute to the increase in IMCL (18). Thus the ability to monitor the IMCL pool and its properties noninvasively by magnetic resonance spectroscopy (MRS) has been an important development (4, 28). The correlation between the magnitude of the IMCL pool, as determined by MRS studies, and insulin resistance, diabetes, and disorders of lipid metabolism has been previously demonstrated (2,3,32,33). Nevertheless, quantification of IMCL and extramyocellular lipid (EMCL) by MRS remains highly problematic (32). The ability to distinguish IMCL from EMCL is based on their different bulk magnetic susceptibility effects due to their geometric arrangements within muscle, which leads to a spectroscopic frequency separation between the two pools; this sepa...

show abstract

“…25 Increased LP products in breast cancer tissues have been demonstrated. 26 Tumor cells have been shown to have abnormal levels of antioxidant enzyme activities when compared with normal cells. 27 Enzyme activities were not evaluated in our study.…”

Section: Discussionmentioning

confidence: 99%

“…Published studies implicate free radicals in cell transformation and in the uncontrolled growth potential of tumor cells. 6,26,28 Among well documented effects, free radicals are known to induce DNA damage and genomic instability favoring the acquisition of mutations that contribute to cellular transformation and cancer cells survival. [6][7]26 ROS modifies redox-sensitive signal transduction pathways that contribute in the cell transformation, cell growth and cancer progression.…”

Section: Discussionmentioning

confidence: 99%

“…6,26,28 Among well documented effects, free radicals are known to induce DNA damage and genomic instability favoring the acquisition of mutations that contribute to cellular transformation and cancer cells survival. [6][7]26 ROS modifies redox-sensitive signal transduction pathways that contribute in the cell transformation, cell growth and cancer progression. 9 Permanent modification of genetic material resulting from the oxidative damage is one of the vital steps involved in mutagenesis that leads to carcinogenesis.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Oxidative stress parameters in women with breast cancer undergoing neoadjuvant chemotherapy and treated with nutraceutical doses of oral glutamine

Farias¹,

Furtado²,

Guimarães³

et al. 2011

Acta Cir. Bras.

View full text Add to dashboard Cite

PURPOSE: To evaluate the effects of oral administration of GLN on the oxidative stress in women with breast cancer undergoing neoadjuvant FAC chemotherapy (5 fluouracil 500 mg/m²+Doxorubicin 50 mg/m²+Cyclophosphamide 500 mg/m² body surface area). METHODS: Twenty women (mean age: 51.7 years) with breast ductal carcinomas classified as T3 or T4 were included in the study, regardless of pre or post menopause status. Sachets containing glutamine 15g ("A") or milk protein 15g ("B") were prepared by a registered pharmacist. Allocation of patients was made by software program. Patients who received sachets labeled "A" were included in G1 group. The remaining patients, treated with the preparation labeled "B", were included in group G2. Sachets contents were blended in 150 ml of drinking water, and were given daily to each patient during the entire course of neoadjuvant chemotherapy. Peripheral blood samples were collected in the first day of each of the three cycles of chemotherapy before drug infusion. Tumor and normal breast samples were collected at the end of Patey´s surgical procedure. Samples were analysed for GSH and TBARS contents. RESULTS: TBARS and GSH values were not different in breast healthy and tumor tissues nor blood when comparing control (G-2) and glutamine-treated (G-1) patients. Also, no significant differences were found in TBARS and GSH levels comparing different timepoints within the same group. CONCLUSION: Oral GLN (15g/kg/day) offers no protection against systemic or local oxidative stress in women with breast Ca undergoing neoadjuvant chemotherapy (FAC).

show abstract

Fatty acid composition of phospholipids and neutral lipids and lipid peroxidation in human breast cancer and lipoma tissue

Cited by 48 publications

References 0 publications

p53 As a mutagen test in breast cancer

p53 As a mutagen test in breast cancer

Distinct patterns of fat metabolism in skeletal muscle of normal-weight, overweight, and obese humans

Oxidative stress parameters in women with breast cancer undergoing neoadjuvant chemotherapy and treated with nutraceutical doses of oral glutamine

Contact Info

Product

Resources

About