Fatty acid desaturase 2 affects the milk‐production traits in Chinese Holsteins

Fu, Yihan; Jia, Ruike; Xu, Lingna; Su, Ding-qiang; Li, Yanhua; Liu, Lin; Ma, Zhuo; Sun, Dan; Han, Bo

doi:10.1111/age.13192

Cited by 8 publications

(8 citation statements)

References 14 publications

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“…In the study of San et al, some genes which affected intramuscular fat (IMF) deposition was significantly enriched in the ECM–receptor interaction signaling pathway ( San et al, 2021 ). In our study, the target genes of upregulated DE miRNAs were enriched in the PI3K-Akt signaling pathway, calcium signaling pathway, the AMPK signaling pathway, and MAPK signaling pathway, which are associated with fat metabolism ( Fu et al, 2022 ). In our study, forkhead boxO3 ( FoxO3 ) was enriched in the PI3K/AKT signaling pathway and AMPK signal pathway.…”

Section: Discussionmentioning

confidence: 88%

MiRNA-Seq reveals key MicroRNAs involved in fat metabolism of sheep liver

Fei

Jin²,

Yuan³

et al. 2023

Front. Genet.

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There is a genetic difference between Hu sheep (short/fat-tailed sheep) and Tibetan sheep (short/thin-tailed sheep) in tail type, because of fat metabolism. Previous studies have mainly focused directly on sheep tail fat, which is not the main organ of fat metabolism. The function of miRNAs in sheep liver fat metabolism has not been thoroughly elucidated. In this study, miRNA-Seq was used to identify miRNAs in the liver tissue of three Hu sheep (short/fat-tailed sheep) and three Tibetan sheep (short/thin-tailed sheep) to characterize the differences in fat metabolism of sheep. In our study, Hu sheep was in a control group, we identified 11 differentially expressed miRNAs (DE miRNAs), including six up-regulated miRNAs and five down-regulated miRNAs. Miranda and RNAhybrid were used to predict the target genes of DE miRNAs, obtaining 3,404 target genes. A total of 115 and 67 GO terms as well as 54 and 5 KEGG pathways were significantly (padj < 0.05) enriched for predicted 3,109 target genes of up-regulated and 295 target genes of down-regulated miRNAs, respectively. oar-miR-432 was one of the most up-regulated miRNAs between Hu sheep and Tibetan sheep. And SIRT1 is one of the potential target genes of oar-miR-432. Furthermore, functional validation using the dual-luciferase reporter assay indicated that the up-regulated miRNA; oar-miR-432 potentially targeted sirtuin 1 (SIRT1) expression. Then, the oar-miR-432 mimic transfected into preadipocytes resulted in inhibited expression of SIRT1. This is the first time reported that the expression of SIRT1 gene was regulated by oar-miR-432 in fat metabolism of sheep liver. These results could provide a meaningful theoretical basis for studying the fat metabolism of sheep.

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Section: Discussionmentioning

confidence: 88%

MiRNA-Seq reveals key MicroRNAs involved in fat metabolism of sheep liver

Fei

Jin²,

Yuan³

et al. 2023

Front. Genet.

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“…As the most complex trait in dairy cow production, milk production trait is regulated by many genes and environmental factors. Some nucleotide polymorphisms may be caused by a large (Fu et al, 2022;Jia et al, 2021;Potu et al, 2022) have discussed that SNPs in milk production candidate functional genes can significantly affect milk production traits of dairy cows. In addition, by adding functional gene information of target traits with large genetic effects to expand SNP marker data, the accuracy of genome breeding value prediction can be improved (de Las et al, 2020;Ye et al, 2022).…”

Section: Discussionmentioning

confidence: 99%

Polymorphism of ADAM12, DPP6 and PRKN genes and their associations with milk production traits in Holstein

Li,

Hu,

Feng

et al. 2023

Reprod Domestic Animals

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Milk production traits as the most important economic traits of dairy cows, they directly reflect the benefits of breeding and the economic benefits of pasture. In this study, A disintegrin and metalloproteinase‐12 (ADAM12), Parkinson's disease gene 2 (PRKN) and dipeptidyl peptidase‐like protein subtype 6 (DPP6) polymorphism in 384 Chinese Holstein cows were detected by time‐of‐flight mass spectrometry and through statistical analysis using software such as Popgene 32, SAS 9.4 and Origin 2022, the relationship between single nucleotide polymorphisms (SNPs) of three genes with four milk production traits such as daily milk yield (DMY), milk fat percentage (MFP), milk protein percentage (MPP) and somatic cell score (SCS) was verified at molecular level. The results showed that four polymorphic loci (116,467,133, 116,604,487, 116,618,268 and 116,835,111) of DPP6 gene, two polymorphic loci (97,665,052 and 97,159,837) of PRKN gene and two polymorphic loci (45,542,714 and 45,553,888) of ADAM12 gene were detected. PRKN‐97665052, DPP6‐116467133, ADAM12‐45553888, DPP6‐116604487 and DPP6‐116835111 were all in Hardy–Weinberg equilibrium state (p > .05). ADAM12‐45542714, PRKN‐97159837 and PRKN‐97665052 were moderately polymorphic (0.25 ≤ PIC <0.50) in Holstein. It is evident that the selection potential and genetic variation of these five loci are relatively large, and the genetic richness is relatively high. The correlation analysis of different genotypes between these eight loci and milk production traits of Holstein showed that ADAM12‐45542714 and DPP6‐116835111 (p < .01) had an extremely significant effects on the DMY of Chinese Holstein in Ningxia, while PRKN‐97665052 had an extremely significant effect on MFP (p < .01). The effect of PRKN‐97665052 and DPP6‐116467133 on MPP of Holstein were extremely significant (p < .01). DPP6‐116618268 had an extremely significant effect on the SCS of Holstein in Ningxia (p < .01), and AA genotype individuals showed a higher SCS than GG genotype individuals; the other two loci (ADAM12‐45553888 and DPP6‐116604487) had no significant effects on milk production traits of Holstein (p > .05). In addition, through the joint analysis of DPP6, PRKN and ADAM12 gene loci, it was found that the interaction effect between the three gene loci could significantly affect the DMY, SCS (p < .01) and MPP (p < .05). In conclusion, several different loci of DPP6, PRKN and ADAM12 genes can affect the milk production traits of Holstein to different degrees. PRKN, DPP6 and ADAM12 genes can be used as potential candidate genes for milk production traits of Holstein for marker‐assisted selection, providing theoretical basis for breeding of Holstein.

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“…Therefore, many researchers try to find the key genes/loci that have significant impacts on milk production traits through candidate gene analysis, genome-wide association analysis and omics data analysis strategies ( Schennink et al, 2009 ; Canovas et al, 2013 ; Cui et al, 2014 ; Liang et al, 2017 ; Bhat et al, 2020 ). Studies have shown that the milk production traits of dairy cows can be significantly affected by SNPs in the genes ( Jiang et al, 2010 ; Dux et al, 2018 ; Han et al, 2019a ; Han et al, 2019b ; Clancey et al, 2019 ; Jia et al, 2021 ; Du et al, 2022 ; Fu et al, 2022 ; Ye et al, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

Genetic marker identification of SEC13 gene for milk production traits in Chinese holstein

Jia

Sun

et al. 2023

Front. Genet.

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SEC13 homolog, nuclear pore and COPII coat complex component (SEC13) is the core component of the cytoplasmic COPII complex, which mediates material transport from the endoplasmic reticulum to the Golgi complex. Our preliminary work found that SEC13 gene was differentially expressed in dairy cows during different stages of lactation, and involved in metabolic pathways of milk synthesis such as citric acid cycle, fatty acid, starch and sucrose metabolisms, so we considered that the SEC13 might be a candidate gene affecting milk production traits. In this study, we detected the polymorphisms of SEC13 gene and verified their genetic effects on milk yield and composition traits in a Chinese Holstein cow population. By sequencing the whole coding and partial flanking regions of SEC13, we found four single nucleotide polymorphisms (SNPs). Subsequent association analysis showed that these four SNPs were significantly associated with milk yield, fat yield, protein yield or protein percentage in the first and second lactations (p ≤.0351). We also found that two SNPs in SEC13 formed one haplotype block by Haploview4.2, and the block was significantly associated with milk yield, fat yield, fat percentage, protein yield or protein percentage (p ≤ .0373). In addition, we predicted the effect of SNP on 5′region on transcription factor binding sites (TFBSs), and found that the allele A of 22:g.54362761A>G could bind transcription factors (TFs) GATA5, GATA3, HOXD9, HOXA10, CDX1 and Hoxd13; and further dual-luciferase reporter assay verified that the allele A of this SNP inhibited the fluorescence activity. We speculate that the A allele of 22:g.54362761A>G might inhibit the transcriptional activity of SEC13 gene by binding the TFs, which may be a cause mutation affecting the formation of milk production traits in dairy cows. In summary, we proved that SEC13 has a significant genetic effect on milk production traits and the identified significant SNPs could be used as candidate genetic markers for GS SNP chips development; on the other hand, we verified the transcriptional regulation of 22:g.54362761A>G on SEC13 gene, providing research direction for further function validation tests.

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Fatty acid desaturase 2 affects the milk‐production traits in Chinese Holsteins

Cited by 8 publications

References 14 publications

MiRNA-Seq reveals key MicroRNAs involved in fat metabolism of sheep liver

MiRNA-Seq reveals key MicroRNAs involved in fat metabolism of sheep liver

Polymorphism of ADAM12, DPP6 and PRKN genes and their associations with milk production traits in Holstein

Genetic marker identification of SEC13 gene for milk production traits in Chinese holstein

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