Fatty acid desaturases (FADs) modulate multiple lipid metabolism pathways to improve plant resistance

Xiao, Ruixue; Zou, Yirong; Guo, Xiao‐Na; Li, Hui; Lu, Hai

doi:10.1007/s11033-022-07568-x

Cited by 37 publications

(12 citation statements)

References 122 publications

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“…The increase in unsaturated FAs content in the cell membrane enhanced the membrane lipid fluidity and thus could increase the tolerance of the cell to the low temperature ( Xiao et al., 2022 ). The results above showed that the overexpression of VfSAD1/2 promoted the production of oleic acid and/or its derivation in the BY4741 and Arabidopsis .…”

Section: Resultsmentioning

confidence: 99%

Tung tree stearoyl‐acyl carrier protein Δ9 desaturase improves oil content and cold resistance of Arabidopsis and Saccharomyces cerevisiae

Chen

Gao²,

Zhang³

et al. 2023

Front. Plant Sci.

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The seed oil of tung tree is rich in a-eleostearic acid (ESA), which endows tung oil with the characteristic of an excellently dry oil. The stearoyl-acyl carrier protein δ9 desaturase (SAD) is a rate-limiting enzyme that converts the stearic acid to the oleic acid, the substrate for the production of the α-ESA. However, the function of the two predicted VfSAD1 and VfSAD2 genes in the tung tree has not been determined. In this study, quantitative real-time PCR (qRT-PCR) analysis showed that VfSAD1 and VfSAD2 were expressed in multiple organs of tung tree but were highly expressed in the seed during the oil rapid accumulation period. Heterologous expression of VfSAD1 and VfSAD2 could promote the production of oleic acid and its derivatives in Arabidopsis thaliana and yeast BY4741, indicating that VfSAD1 and VfSAD2 possess the stearoyl-ACP desaturases function. Furthermore, both VfSAD1 and VfSAD2 could significantly improve seed oil accumulation in Arabidopsis. VfSAD1 could also significantly promote the oil accumulation in the yeast BY4741 strain. In addition, overexpression of VfSAD1 and VfSAD2 enhanced the tolerance of yeast and Arabidopsis seedlings to low temperature stress. This study indicates that the two VfSAD genes play a vital role in the process of oil accumulation and fatty acid biosynthesis in the tung tree seed, and both of them could be used for molecular breeding in tung tree and other oil crops.

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Section: Resultsmentioning

confidence: 99%

Tung tree stearoyl‐acyl carrier protein Δ9 desaturase improves oil content and cold resistance of Arabidopsis and Saccharomyces cerevisiae

Chen

Gao²,

Zhang³

et al. 2023

Front. Plant Sci.

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“…The fatty acid desaturase 2-9 (FAD2-9) was significantly induced (10.51 Log fold). FADs are known to regulate ROS signaling and fluidity of cell membranes during defense (Xiao et al, 2022). Some genes involved in cell death such as the gene encoding biotic cell death associated protein (Solyc03g098740) was upregulated and a gene that encodes serpin (Solyc04g079470) was downregulated.…”

Section: Resultsmentioning

confidence: 99%

The Sw-5b NLR immune receptor induces earlier transcriptional changes in response to thrips-mediated inoculation of Tomato spotted wilt orthotospovirus compared to mechanical inoculation

Ordaz

Nagalakshmi

Boiteux

et al. 2022

Preprint

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The nucleotide-binding leucine-rich repeat (NLR) class of immune receptor, Sw-5b confers resistance to Tomato spotted wilt orthotospovirus (TSWV). Although Sw-5b is known to activate immunity upon recognition of the NSm of TSWV, we know very little about the downstream events that lead to resistance. Here, we investigated the early transcriptomic changes that occur in response to both mechanical and thrips-mediated inoculation of TSWV using near-isogenic resistant and susceptible tomato lines. Interestingly, Sw-5b induces earlier transcriptional changes in response to thrips-mediated inoculation compared to mechanical inoculation of TSWV. A subset of the differentially expressed genes (DEGs) observed at 12 and 24 hours post thrips-mediated inoculation of TSWV was only present at 72 hours post mechanical inoculation. Although some DEGs were shared between thrips and mechanical inoculation at 72 hours post-infection, many DEGs were specific to either thrips-mediated or mechanical inoculation of TSWV. In response to thrips-mediated inoculation, an NLR immune receptor, cysteine-rich receptor-like kinase, G-type lectin S-receptor-like kinases, and transcription factors such as the ethylene response factor 1 and the calmodulin-binding protein 60 were induced. Whereas, in response to mechanical inoculation, fatty acid desaturase 2-9, cell death genes, DCL2b, RIPK/PBL14-like, and transcription factors such as ERF017 and WRKY75 were differentially expressed. Our findings reveal novel insights into Sw-5b responses specific to the method of TSWV inoculation. Given that TSWV is transmitted in nature primarily by the thrips, the DEGs we have identified provide a foundation for understanding the mechanistic roles of these genes in the Sw-5b-mediated resistance.

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“…StLTP10 -overexpressing improve the expression of disease-resistant genes, and reduce oxidative stress to enhance the resistance to Phytophthora infestans ( Wang et al., 2021 ). Fatty acid desaturation (FAD) catalyzes the formation of unsaturated fatty acids (UFA), which is a precursor for the biosynthesis of various hormones, such as JA, SA and terpenes ( Xiao et al., 2022 ). The fad3fad7fad8 triple mutant of Arabidopsis impaired SA synthesis, resulting in an enhanced sensitivity to oomycetes ( Browse, 2009 ).…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic and functional analyses reveal the molecular mechanisms underlying Fe-mediated tobacco resistance to potato virus Y infection

Guo

et al. 2023

Front. Plant Sci.

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Potato virus Y (PVY) mainly infects Solanaceous crops, resulting in considerable losses in the yield and quality. Iron (Fe) is involved in various biological processes in plants, but its roles in resistance to PVY infection has not been reported. In this study, foliar application of Fe could effectively inhibit early infection of PVY, and a full-length transcriptome and Illumina RNA sequencing was performed to investigate its modes of action in PVY-infected Nicotiana tabacum. The results showed that 18,074 alternative splicing variants, 3,654 fusion transcripts, 3,086 long non-coding RNAs and 14,403 differentially expressed genes (DEGs) were identified. Specifically, Fe application down-regulated the expression levels of the DEGs related to phospholipid hydrolysis, phospholipid signal, cell wall biosynthesis, transcription factors (TFs) and photosystem I composition, while those involved with photosynthetic electron transport chain (PETC) were up-regulated at 1 day post inoculation (dpi). At 3 dpi, these DEGs related to photosystem II composition, PETC, molecular chaperones, protein degradation and some TFs were up-regulated, while those associated with light-harvesting, phospholipid hydrolysis, cell wall biosynthesis were down-regulated. At 9 dpi, Fe application had little effects on resistance to PVY infection and transcript profiles. Functional analysis of these potentially critical DEGs was thereafter performed using virus-induced gene silencing approaches and the results showed that NbCat-6A positively regulates PVY infection, while the reduced expressions of NbWRKY26, NbnsLTP, NbFAD3 and NbHSP90 significantly promote PVY infection in N. benthamiana. Our results elucidated the regulatory network of Fe-mediated resistance to PVY infection in plants, and the functional candidate genes also provide important theoretical bases to further improve host resistance against PVY infection.

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Fatty acid desaturases (FADs) modulate multiple lipid metabolism pathways to improve plant resistance

Cited by 37 publications

References 122 publications

Tung tree stearoyl‐acyl carrier protein Δ9 desaturase improves oil content and cold resistance of Arabidopsis and Saccharomyces cerevisiae

Tung tree stearoyl‐acyl carrier protein Δ9 desaturase improves oil content and cold resistance of Arabidopsis and Saccharomyces cerevisiae

The Sw-5b NLR immune receptor induces earlier transcriptional changes in response to thrips-mediated inoculation of Tomato spotted wilt orthotospovirus compared to mechanical inoculation

Transcriptomic and functional analyses reveal the molecular mechanisms underlying Fe-mediated tobacco resistance to potato virus Y infection

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