Fatty acid ethyl esters production using a non-commercial lipase in pressurized propane medium

Hildebrand, Cristiane; Rosa, Clarissa Dalla; Freire, Denise Maria Guimarães; Destain, Jean‐Pierre; Dariva, Cláudio; Oliveira, Débora de; Oliveira, J. Vladimir

doi:10.1590/s0101-20612009000300023

Cited by 7 publications

(2 citation statements)

References 37 publications

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“…Another series of experiments was carried out with 1:3 molar ratio and varying the reaction temperature between 35 and 65°C (Table ). The optimal temperature appeared to be 45°C, which was consistent with previous studies …”

Section: Resultssupporting

confidence: 87%

Lipase‐catalyzed synthesis of sorbitol octanoate in aqueous biphasic medium and its use in a green formulation process of oil‐in‐water food nanoemulsions

Wongthongdee

Durand

Pongtharangkul

et al. 2017

J of Chemical Tech & Biotech

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BACKGROUND: Sugar-based surfactants are highly relevant alternative ingredients for food grade formulations. Nevertheless, the design of sustainable manufacturing processes is still ongoing. RESULTS: Sorbitol ester surfactants were synthesized by lipase-catalyzed esterification in solvent-free conditions. Octanoic acid was dispersed in a 70 wt% sorbitol aqueous solution (containing the enzyme). The maximal conversion of 23.5 mole % of esterified fatty acid per mole of loaded fatty acid was obtained after 48 h in optimal conditions. The performance of the reactor was affected by both the nature and amount of the reactants and the dispersion state. Detailed structural analysis demonstrated that lipase from Candida rugosa specifically catalyzed the acylation of sorbitol on primary hydroxyl groups. Sorbitol esters accumulated exclusively in the oil phase, which led to easy and efficient product recovery. Oil phase containing the sorbitol esters could be used directly for preparing oil-in-water nanoemulsion without adding any other stabilizer. These nanoemulsions exhibited good stability after 7 days storage at 25 ∘ C or 60 ∘ C. CONCLUSION: A green manufacturing process for food grade oil-in-water nanoemulsions was designed involving a lipase-catalyzed esterification step which produced in situ the required surfactant. Nanoemulsions were prepared without using any other stabilizer. Biocatalyst reusabilityA reusability study of lipase was carried out by consecutive batch reactions with an initial load containing 0.94 g sorbitol (5.2 mmol), 1.487 g octanoic acid (1.25 mmol), 0.34 g lipase (AY Amano) and 400 L water. The total volume of the reaction medium was around

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Section: Resultssupporting

confidence: 87%

Lipase‐catalyzed synthesis of sorbitol octanoate in aqueous biphasic medium and its use in a green formulation process of oil‐in‐water food nanoemulsions

Wongthongdee

Durand

Pongtharangkul

et al. 2017

J of Chemical Tech & Biotech

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“…Despite of enzyme activity losses and lower conversions (up to 63.2% with Novozym 435), supercritical carbon dioxide showed to be an appropriate solvent for enzymatic alcoholysis. Other studies involving lipase-catalyzed in pressurized fluids indicated this process as a potential technology for biodiesel production (Hildebrand et al, 2009). According to Oliveira et al (2006), the enzyme behaviour in compressed fluids is of primary importance as the loss of enzyme activity may lead to undesirable poor reaction rates and low yields of target products.…”

Section: Enzymatic Reactions In Compressed and Supercritical Fluidsmentioning

confidence: 99%

An Overview of Enzyme-Catalyzed Reactions and Alternative Feedstock for Biodiesel Production

Feltes¹,

Oliveira²,

Ninow³

et al. 2011

Alternative Fuel

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Progress in Supercritical Fluid Technology for Fats and Oils Processing

Temelli

Güçlü‐Üstündağ

Couto

2020

Bailey's Industrial Oil and Fat Products

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Supercritical CO 2 (SC‐CO 2 ) processing of fats and oils has been widely investigated since SC‐CO 2 offers an environmentally friendly alternative with added advantages such as moderate operating conditions and solvent‐free extracts and residues. From a processing perspective, a unique advantage of SC‐CO 2 processing lies in its versatility, which results from the ability to modify solvent properties by changing operating conditions (temperature and pressure) or by the addition of cosolvents. A good understanding of the fundamentals of solubility behavior of lipid components in SC‐CO 2 as affected by operating conditions and solute properties is required to realize its full potential in fats and oils processing. The operational flexibility offered by supercritical fluid technology enables the processor to fine‐tune solvent properties and to develop novel processes by the integration of unit operations of extraction, fractionation, reaction, and particle formation to meet the process objectives. These unit operations have been effectively utilized for the extraction and refining of oils, concentration of bioactive components from oils or oil by‐products, modification of the physical properties of fats and oils, production of oleochemicals and design of delivery systems for numerous applications.

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Fatty acid ethyl esters production using a non-commercial lipase in pressurized propane medium

Cited by 7 publications

References 37 publications

Lipase‐catalyzed synthesis of sorbitol octanoate in aqueous biphasic medium and its use in a green formulation process of oil‐in‐water food nanoemulsions

Lipase‐catalyzed synthesis of sorbitol octanoate in aqueous biphasic medium and its use in a green formulation process of oil‐in‐water food nanoemulsions

An Overview of Enzyme-Catalyzed Reactions and Alternative Feedstock for Biodiesel Production

Progress in Supercritical Fluid Technology for Fats and Oils Processing

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