Fatty acid incorporation in endothelial cells and effects on endothelial nitric oxide synthase

Couloubaly, Samba; Deloménie, Claudine; Rousseau, Delphine; Paul, Jean–Louis; Grynberg, Alain; Pourci, M.L.

doi:10.1111/j.1365-2362.2007.01843.x

Cited by 18 publications

(12 citation statements)

References 45 publications

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“…Obese subjects also have elevated levels of FFAs, which have been demonstrated to increase levels of oxidative stress and proinflammatory signaling and impair endothelial function (33). Further, there is evidence that increased levels of particular FFAs may directly inhibit endothelium‐derived NO synthase activity and its phosphorylation (34). Microvascular function is, however, only partially NO‐dependent, with several other mediators playing an important role (22).…”

Section: Discussionmentioning

confidence: 99%

The relationship between anthropometric indexes of adiposity and vascular function in the FATE cohort

Martin

Verma

Charbonneau

et al. 2013

Obesity

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Objective: Numerous indexes of adiposity have been proposed and are currently in use in clinical practice and research. However, the correlation of these indexes with measures of vascular health remain poorly defined. This study investigated which measure of adiposity is most strongly associated with endothelial function. Design and Methods: Data from the Firefighters And Their Endothelium (FATE) study was used. The relationships between three measures of vascular function: flow-mediated dilation (FMD), hyperemic velocity time integral (VTI), and hyperemic shear stress (HSS), and five measures of adiposity: BMI, waist circumference (WC), waist-to-hip ratio (WHR), waist-to-height ratio (WHtR), and body adiposity index (BAI) were tested. Univariate comparisons were made, and subsequently models adjusted for traditional risk factors were constructed. Results: A total of 1,462 male firefighters (mean age 49 6 9) without cardiovascular disease comprised the study population. No measure of adiposity correlated with FMD; all five measures of adiposity were negatively correlated with VTI and HSS (P values <0.0001), with WHtR most strongly correlated with VTI, and WC most strongly correlated with HSS (both P < 0.05). In models including all five measures of obesity simultaneously, BMI, WC, and WHtR were all predictive of HSS (all P values <0.05), and BMI and WHR were both predictive of VTI (P values <0.05). Conclusions: Anthropometric measures of adiposity may help refine estimations of atherosclerotic burden. BMI was most consistently associated with endothelial dysfunction, but measures of adiposity that reflect distribution of mass were additive.

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Section: Discussionmentioning

confidence: 99%

The relationship between anthropometric indexes of adiposity and vascular function in the FATE cohort

Martin

Verma

Charbonneau

et al. 2013

Obesity

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“…eNOS activity is modulated by the fatty acid composition of cell membranes and of caveolae [43], [44]. Thus induced changes in arterial membrane fatty acid composition may alter eNOS activity by changing the biophysical properties of arterial cell membranes and so contribute to differences in vaso-relaxation between maternal dietary groups.…”

Section: Discussionmentioning

confidence: 99%

Vascular Dysfunction Induced in Offspring by Maternal Dietary Fat Involves Altered Arterial Polyunsaturated Fatty Acid Biosynthesis

et al. 2012

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Nutrition during development affects risk of future cardiovascular disease. Relatively little is known about whether the amount and type of fat in the maternal diet affect vascular function in the offspring. To investigate this, pregnant and lactating rats were fed either 7%(w/w) or 21%(w/w) fat enriched in either18:2n-6, trans fatty acids, saturated fatty acids, or fish oil. Their offspring were fed 4%(w/w) soybean oil from weaning until day 77. Type and amount of maternal dietary fat altered acetylcholine (ACh)-mediated vaso-relaxation in offspring aortae and mesenteric arteries, contingent on sex. Amount, but not type, of maternal dietary fat altered phenylephrine (Pe)-induced vasoconstriction in these arteries. Maternal 21% fat diet decreased 20:4n-6 concentration in offspring aortae. We investigated the role of Δ6 and Δ5 desaturases, showing that their inhibition in aortae and mesenteric arteries reduced vasoconstriction, but not vaso-relaxation, and the synthesis of specific pro-constriction eicosanoids. Removal of the aortic endothelium did not alter the effect of inhibition of Δ6 and Δ5 desaturases on Pe-mediated vasoconstriction. Thus arterial smooth muscle 20:4n-6 biosynthesis de novo appears to be important for Pe-mediated vasoconstriction. Next we studied genes encoding these desaturases, finding that maternal 21% fat reduced Fads2 mRNA expression and increased Fads1 in offspring aortae, indicating dysregulation of 20:4n-6 biosynthesis. Methylation at CpG −394 bp 5′ to the Fads2 transcription start site predicted its expression. This locus was hypermethylated in offspring of dams fed 21% fat. Pe treatment of aortae for 10 minutes increased Fads2, but not Fads1, mRNA expression (76%; P<0.05). This suggests that Fads2 may be an immediate early gene in the response of aortae to Pe. Thus both amount and type of maternal dietary fat induce altered regulation of vascular tone in offspring though differential effects on vaso-relaxation, and persistent changes in vasoconstriction via epigenetic processes controlling arterial polyunsaturated fatty acid biosynthesis.

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“…This is a highly regulated process that requires leukocytes to the endothelium requires the interaction of integrin ligands on the leukocyte surface through immunoglobulin superfamily members, i.e., ICAM-1, ICAM-2 and VCAM-1, expressed on the endothelium [27]. Effects of OLA and LNA on endothelial cell expression of integrin ligands are conflicting: some authors demonstrated increases [15,28] and others found decreases in the expression of adhesion molecules [29][30][31]. These contrasting results possibly occurred due to differences in the experimental conditions such as the cell type, time of incubation and fatty acid concentrations used.…”

Section: Discussionmentioning

confidence: 99%

Dietary Free Oleic and Linoleic Acid Enhances Neutrophil Function and Modulates the Inflammatory Response in Rats

Rodrigues

Vinolo

Magdalon

et al. 2010

Lipids

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The high ingestion of oleic (OLA) and linoleic (LNA) acids by Western populations, the presence of inflammatory diseases in these populations, and the importance of neutrophils in the inflammatory process led us to investigate the effects of oral ingestion of unesterified OLA and LNA on rat neutrophil function. Pure OLA and LNA were administered by gavage over 10 days. The doses used (0.11, 0.22 and 0.44 g/kg of body weight) were based on the Western consumption of OLA and LNA. Neither fatty acid affected food, calorie or water intake. The fatty acids were not toxic to neutrophils as evaluated by cytometry using propidium iodide (membrane integrity and DNA fragmentation). Neutrophil migration in response to intraperitoneal injection of glycogen and in the air pouch assay, was elevated after administration of either OLA or LNA. This effect was associated with enhancement of rolling and increased release of the chemokine CINC-2alphabeta. Both fatty acids elevated L-selectin expression, whereas no effect on beta(2)-integrin expression was observed, as evaluated by flow cytometry. LNA increased the production of proinflammatory cytokines (IL-1beta and CINC-2alphabeta) by neutrophils after 4 h in culture and both fatty acids decreased the release of the same cytokines after 18 h. In conclusion, OLA and LNA modulate several functions of neutrophils and can influence the inflammatory process.

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Fatty acid incorporation in endothelial cells and effects on endothelial nitric oxide synthase

Cited by 18 publications

References 45 publications

The relationship between anthropometric indexes of adiposity and vascular function in the FATE cohort

The relationship between anthropometric indexes of adiposity and vascular function in the FATE cohort

Vascular Dysfunction Induced in Offspring by Maternal Dietary Fat Involves Altered Arterial Polyunsaturated Fatty Acid Biosynthesis

Dietary Free Oleic and Linoleic Acid Enhances Neutrophil Function and Modulates the Inflammatory Response in Rats

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