Fatty acid metabolism of isolated mammalian cells

Rosenthal, Miriam D.

doi:10.1016/0163-7827(87)90009-9

Cited by 155 publications

(57 citation statements)

References 185 publications

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“…The level of C-18:2n-6 in the diet also influences, to some extent, the concentration of other n-6 fatty acids. Diets rich in linoleic acid increase the levels of longer chain length n-6 fatty acids, mainly arachidonic acid (Lestingi, Laudadio, Marisco, Carola, & Vicente, 2004) because these fatty acids can be synthesized from dietary linoleic acid (Rosenthal, 1987). This effect may explain the higher levels of arachidonic acid in turtle dove meat.…”

Section: Discussionmentioning

confidence: 89%

Characterization of meat from two game birds: thrush (Turdus philomelos) and turtle dove (Streptopelia turtur) Caracterización de la carne de dos aves de caza: zorzal (Turdus philomelos) y tórtola (Streptopelia turtur)

Rodríguez-Turienzo

Dı́az

Sanmartín

et al. 2010

CyTA - Journal of Food

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The chemical and fatty acid composition and color of wild thrush (Turdus philomelos) and turtle dove (Streptopelia turtur) meat were investigated. No significant differences in the chemical composition were observed between the meat of thrush and that of turtle dove. However, significant differences (p 5 0.05) in the fatty acid composition of the three fractions (glycerides, phospholipids, and free fatty acids) and in color parameters were observed. The main fatty acid in thrush meat was oleic acid (around 30%), and the high content of docosahexaenoic acid (C-22:6n-3) (more than 10% in the three fractions) was remarkable. There was a high content of polyunsaturated fatty acids (PUFA) (more than 50%) in the turtle dove meat; the main PUFA were linoleic (C-18:2n-6) and arachidonic (C-20:4n-6) acids.Keywords: game; thrush; turtle dove; meat; lipids; fatty acids; color Se ha estudiado la composicio´n quı´mica, de a´cidos grasos y el color de la carne de zorzal (Turdus philomelos) y to´rtola (Streptopelia turtur). No se observaron diferencias significativas en la composicio´n quı´mica entre la carne de ambas especies; sin embargo, sı´se detectaron diferencias significativas (p 5 0,05) en la composicio´n de a´cidos grasos de las tres fracciones lipı´dicas (glice´ridos, fosfolı´pidos y a´cidos grasos libres) y en los para´metros de color. El principal a´cido graso en la carne de zorzal fue el a´cido oleico (alrededor del 30%), siendo remarcable el alto contenido de a´cido docosahexaenoico (C-22:6n-3) (mayor del 10% en las tres fracciones). La carne de to´rtola presento´un alto contenido de a´cidos grasos poliinsaturados (mayor del 50%), siendo los principales a´cidos grasos poliinsaturados el linoleico (C-18:2n-6) y el araquido´nico (C-20:4n-6).

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Section: Discussionmentioning

confidence: 89%

Characterization of meat from two game birds: thrush (Turdus philomelos) and turtle dove (Streptopelia turtur) Caracterización de la carne de dos aves de caza: zorzal (Turdus philomelos) y tórtola (Streptopelia turtur)

Rodríguez-Turienzo

Dı́az

Sanmartín

et al. 2010

CyTA - Journal of Food

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“…The present study was performed under conditions where neither cell line was metabolically predisposed to storing lipid. 23 This is perhaps obvious with EPC-EFAD cells, being cultured in delipidated FBS, but even culture in 10% FBS does not lead to the deposition of lipid in the form of lipid droplets in the EPC cell line and so levels of page 14…”

Section: Discussionmentioning

confidence: 99%

Effects of essential fatty acid deficiency and supplementation with docosahexaenoic acid (DHA; 22:6n-3) on cellular fatty acid compositions and fatty acyl desaturation in a cell culture model

Tocher

Dick

2001

Prostaglandins, Leukotrienes and Essential Fatty Acids (PLEFA)

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“…The EFAs can theoretically undergo stepwise two-carbon chain elongations or shortenings, as well as desaturations or saturations, leading to a variety of FAs of the same family ( Figure 1). Although processing of this type is believed to occur primarily in the liver, the desaturating and elongating ability of a variety of primary cell cultures and cell lines has been demonstrated (reviewed by Rosenthal, 1987). While many transformed or malignant cells have limited capacity to perform FA desaturations (Dunbar & Bailey, 1975;Iturralde et al, 1990;Marra & de Alaniz, 1992;Naval et al, 1993), very little is known about the ability of cancerous and normal human mammary epithelial cells to elongate and/or desaturate exogenous FAs in culture.…”

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confidence: 99%

n-3 and n-6 fatty acid processing and growth effects in neoplastic and non-cancerous human mammary epithelial cell lines

Grammatikos¹,

Subbaiah²,

Victor³

et al. 1994

Br J Cancer

144

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The type rather than the amount of dietary fat may be more important in breast carcinogenesis. While animal studies support this view, little is known about the effects of essential fatty acids (EFAs) at the cellular level. The MCF-7 breast cancer and the MCF-10A non-cancerous human mammary epithelial cell lines are compared in terms of growth response to EFAs and ability to incorporate and process the EFAs. Eicosapentaenoic (EPA, n-3) and docosahexaenoic (DHA, n-3) acids, presented bound to albumin, inhibited the growth of MCF-7 cells by as much as 50% in a dose-dependent manner (6-30 microM) in medium containing 0.5% serum. alpha-Linolenic (LNA, n-3) and arachidonic (AA, n-6) acids inhibited growth less extensively, while linoleic acid (LA, n-6) had no effect. In contrast, MCF-10A cells were not inhibited by any of the EFAs at levels below 24 microM. The differential effects of AA, EPA and DHA on MCF-7 and MCF-10A cells support a protective role of highly unsaturated essential fatty acids against breast cancer. The EFAs were primarily incorporated into phosphoglycerides. MCF-7 cells showed chain elongations and possibly delta 8 desaturation, but no AA was formed from LA, nor EPA or DHA from LNA. In contrast, MCF-10A cells desaturated and elongated the exogenous EFAs via all the known pathways. These findings suggest defects in the desaturating enzymes of MCF-7 cells. LNA, DHA and AA presented to MCF-7 cells in phospholipid liposomes inhibited growth as extensively as albumin-bound free acids, but were less extensively incorporated, suggesting different mechanisms of inhibition for the two methods.

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Fatty acid metabolism of isolated mammalian cells

Cited by 155 publications

References 185 publications

Characterization of meat from two game birds: thrush (Turdus philomelos) and turtle dove (Streptopelia turtur) Caracterización de la carne de dos aves de caza: zorzal (Turdus philomelos) y tórtola (Streptopelia turtur)

Characterization of meat from two game birds: thrush (Turdus philomelos) and turtle dove (Streptopelia turtur) Caracterización de la carne de dos aves de caza: zorzal (Turdus philomelos) y tórtola (Streptopelia turtur)

Effects of essential fatty acid deficiency and supplementation with docosahexaenoic acid (DHA; 22:6n-3) on cellular fatty acid compositions and fatty acyl desaturation in a cell culture model

n-3 and n-6 fatty acid processing and growth effects in neoplastic and non-cancerous human mammary epithelial cell lines

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