Fatty acid production by four strains of Mucor hiemalis grown in plant oil and soluble carbohydrates

In zygomycetes fungi, many Mucor spp. have been known to produce g-linolenic acid (GLA) in their biomass. Among 250 soil samples screened, 20 Mucor isolates showed GLA in their mycelial mass under normal cultivation conditions. Sudan Black B was used for screening their qualitative oleaginesity. Among the representative isolates, Mucor sp. CFR-G15, when grown in a fat-producing medium, showed a maximum lipid content of 30 6 1.32% in its mycelia and 14.42 6 0.74% GLA. By using gene-specific primers, the 18S rRNA gene and the D 6 DES gene were amplified by PCR technique. The nucleotide sequences of the 18S rRNA and D 6 DES genes exhibited .98% homology with M. rouxii ATCC 24905 (accession nos. AF117923 and AF296076, respectively), suggesting taxonomic identity. The native isolate M. rouxii CFR-G15 reported in this study was found to be promising for the development of an economical process in the industrial production of GLA.

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Identification and characterization of the n‐6 fatty acid‐producing Mucor rouxii native isolate CFR‐G15

Mamatha

Halami

Venkateswaran

2010

Euro J Lipid Sci & Tech

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Differential expression of desaturase genes and changes in fatty acid composition ofMortierellasp. AGED in response to environmental factors

Tan

Zhuo

et al. 2016

J. Sci. Food Agric.

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Application of Response Surface Method for Studying the Role of Dissolved Oxygen and Agitation Speed on Gamma-Linolenic Acid Production

Ahmed¹,

Singh²,

Pandey³

et al. 2008

Appl Biochem Biotechnol

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To study the effect of agitation speed (rpm) and dissolved oxygen concentration (DO) on the production of gamma linolenic acid by Mucor sp. RRL001, a central composite design experiment was performed in a 5-L stirred tank bioreactor. The design consisted of a total of 10 runs consisting of runs at five levels for each factor and was divided in two blocks. The ANOVA analysis and Pareto chart of effects suggested agitation speed (p = 0.0142) linear effect and DO concentration (p = 0.0342) quadratic effects were significant factors with significant contribution to the response. The validation run based on the optimum production zone in response surface plot resulted in the maximum 350.3 mg l(-1) GLA yield as compared with model predicted value of 340.7 mg l(-1). The study suggests that agitation rate is having more pronounced effect on GLA yield than dissolved oxygen concentration by ensuring enhanced mass transfer and by preventing wall growth at elevated agitation speed. Also, it shows that higher GLA yields can be obtained in a simple medium at moderate oxygen saturation and that the Mucor sp. RRL001 is resistant to high agitation linked shear stress and suitable for GLA production at higher scale.

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Fatty acid production by four strains of Mucor hiemalis grown in plant oil and soluble carbohydrates

Abstract: ) media, respectively. Strain M4 produced greater quantities of polyunsaturated acids in medium containing glucose. The GLA production in the M4 biomass was 1,132.2 mg l -1 in glucose medium. Plant oils were inhibitors of fatty acid production by these strains.

Cited by 9 publications

References 17 publications

Identification and characterization of the n‐6 fatty acid‐producing Mucor rouxii native isolate CFR‐G15

Identification and characterization of the n‐6 fatty acid‐producing Mucor rouxii native isolate CFR‐G15

Differential expression of desaturase genes and changes in fatty acid composition ofMortierellasp. AGED in response to environmental factors

Application of Response Surface Method for Studying the Role of Dissolved Oxygen and Agitation Speed on Gamma-Linolenic Acid Production

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