Fatty acid profile of blood plasma and oviduct and uterine fluid during early and late luteal phase in the horse

Drews, Barbara; Milojevic, Vladimir; Giller, Katrin; Ulbrich, Susanne E.

doi:10.1016/j.theriogenology.2018.04.003

Cited by 12 publications

(6 citation statements)

References 84 publications

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“…The uterine fluid AA changes occurred independent from the maternal plasma AA. Similar findings have previously been reported for fatty acids in the plasma, oviductal, and uterine fluid of the mare ( 37 ). Thus, the uterine microenvironment does not represent a mere transudate of the blood ( 38 , 39 ) but is regulated separately.…”

Section: Discussionsupporting

confidence: 91%

Amino acids activate mTORC1 to release roe deer embryos from decelerated proliferation during diapause

Weijden

Bick

Bauersachs

et al. 2021

Proc. Natl. Acad. Sci. U.S.A.

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Embryonic diapause in mammals leads to a reversible developmental arrest. While completely halted in many species, European roe deer (Capreolus capreolus) embryos display a continuous deceleration of proliferation. During a 4-mo period, the cell doubling time is 2 to 3 wk. During this period, the preimplantation blastocyst reaches a diameter of 4 mm, after which it resumes a fast developmental pace to subsequently implant. The mechanisms regulating this notable deceleration and reacceleration upon developmental resumption are unclear. We propose that amino acids of maternal origin drive the embryonic developmental pace. A pronounced change in the abundance of uterine fluid mTORC1-activating amino acids coincided with an increase in embryonic mTORC1 activity prior to the resumption of development. Concurrently, genes related to the glycolytic and phosphate pentose pathway, the TCA cycle, and one carbon metabolism were up-regulated. Furthermore, the uterine luminal epithelial transcriptome indicated increased estradiol-17β signaling, which likely regulates the endometrial secretions adapting to the embryonic needs. While mTORC1 was predicted to be inactive during diapause, the residual embryonic mTORC2 activity may indicate its involvement in maintaining the low yet continuous proliferation rate during diapause. Collectively, we emphasize the role of nutrient signaling in preimplantation embryo development. We propose selective mTORC1 inhibition via uterine catecholestrogens and let-7 as a mechanism regulating slow stem cell cycle progression.

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Section: Discussionsupporting

confidence: 91%

Amino acids activate mTORC1 to release roe deer embryos from decelerated proliferation during diapause

Weijden

Bick

Bauersachs

et al. 2021

Proc. Natl. Acad. Sci. U.S.A.

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“…The Fatty Acid (FA) profile was determined by gas chromatography (GC). Serum samples were processed following the lipid extraction procedure described by Drews et al 2018 [ 32 ]. Briefly, to 25 µL of each serum sample 500 µL of methanol:MTBE (Methyl tert-butyl ether):cholorform 1.33:1:1 v / v / v were added.…”

Section: Methodsmentioning

confidence: 99%

A Mixture of Algae and Extra Virgin Olive Oils Attenuates the Cardiometabolic Alterations Associated with Aging in Male Wistar Rats

et al. 2020

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Aging is one of the major risk factors for suffering cardiovascular and metabolic diseases. Due to the increase in life expectancy, there is a strong interest in the search for anti-aging strategies to treat and prevent these aging-induced disorders. Both omega 3 polyunsaturated fatty acids (ω-3 PUFA) and extra virgin olive oil (EVOO) exert numerous metabolic and cardiovascular benefits in the elderly. In addition, EVOO constitutes an interesting ingredient to stabilize ω-3 PUFA and decrease their oxidation process due to its high content in antioxidant compounds. ω-3 PUFA are commonly obtained from fish. However, more ecological and sustainable sources, such as algae oil (AO) can also be used. In this study, we aimed to study the possible beneficial effect of an oil mixture composed by EVOO (75%) and AO (25%) rich in ω-3 PUFA (35% docosahexaenoic acid (DHA) and 20% eicosapentaenoic acid (EPA)) on the cardiometabolic alterations associated with aging. For this purpose; young (three months old) and old (24 months old) male Wistar rats were treated with vehicle or with the oil mixture (2.5 mL/kg) for 21 days. Treatment with the oil mixture prevented the aging-induced increase in the serum levels of saturated fatty acids (SFA) and the aging-induced decrease in the serum concentrations of mono-unsaturated fatty acids (MUFA). Old treated rats showed increased serum concentrations of EPA and DHA and decreased HOMA-IR index and circulating levels of total cholesterol, insulin and IL-6. Treatment with the oil mixture increased the mRNA levels of antioxidant and insulin sensitivity-related enzymes, as well as reduced the gene expression of pro-inflammatory markers in the liver and in cardiac and aortic tissues. In addition, the treatment also prevented the aging-induced endothelial dysfunction and vascular insulin resistance through activation of the PI3K/Akt pathway. Moreover, aortic rings from old rats treated with the oil mixture showed a decreased response to the vasoconstrictor AngII. In conclusion, treatment with a mixture of EVOO and AO improves the lipid profile, insulin sensitivity and vascular function in aged rats and decreases aging-induced inflammation and oxidative stress in the liver, and in the cardiovascular system. Thus, it could be an interesting strategy to deal with cardiometabolic alterations associated with aging.

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“…From ovulation to implantation, oocytes and embryos are supported by maternal secretions from the oviduct and uterus (cow [ 1 ], sheep [ 2 ], murine [ 3 ], horse [ 4 ], sow [ 5 ] and rabbit [ 6 ]). The oviductal and uterine fluids mainly originate from blood filtrates [ 7 ] and secretions of endometrial luminal and glandular epithelial cells [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

Fatty Acid Profile of Blood Plasma at Mating and Early Gestation in Rabbit

Hadjadj

Hankele

Armero

et al. 2021

Animals

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The aim of this study was to analyse the fatty acid (FA) profile of blood plasma at mating and 72 hpm by gas chromatography. Moreover, the correlation between FA and ovulation rate, normal embryos and compacted morulae was estimated. Palmitic, linoleic, oleic and stearic were the highest FA concentrations at mating and 72 hpm. Most long chain saturated and PUFA were higher at 72 hpm than at mating, while MUFA were higher at mating. SFA, MUFA and PUFA were high and positively correlated. Correlation was 0.643 between MUFA at mating and ovulation rate, and 0.781 between MUFA and normal embryos, respectively. Compacted morulae were slightly correlated with SFA at mating (0.465). In conclusion, the FA profile of plasma varies depending on the reproductive cycle of the rabbit female, adapting to energetic requirements at mating and early gestation. Moreover, positive correlations are found between fatty acids and ovulation rate and embryo development and quality.

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Fatty acid profile of blood plasma and oviduct and uterine fluid during early and late luteal phase in the horse

Cited by 12 publications

References 84 publications

Amino acids activate mTORC1 to release roe deer embryos from decelerated proliferation during diapause

Amino acids activate mTORC1 to release roe deer embryos from decelerated proliferation during diapause

A Mixture of Algae and Extra Virgin Olive Oils Attenuates the Cardiometabolic Alterations Associated with Aging in Male Wistar Rats

Fatty Acid Profile of Blood Plasma at Mating and Early Gestation in Rabbit

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