Fatty acid profiles of Vibrio parahaemolyticus and its changes with environment

Abstract

The fatty acid (FA) profiles of 15 strains representing four genera (Vibrio, Pseudomonas, Aeromonas, and Shewanella) and seven species were compared by capillary gas chromatography with flame ionization detection. FA fingerprints of Vibrio parahaemolyticus were established by similarity calculation and principle component analysis. This provided a simple measure for distinguishing V. parahaemolyticus from other bacteria. The similarity scores indicated by correlation coefficient and vector cosine were divided into three regions and suggested that the strains with scores higher than 0.980 probably belonged to V. parahaemolyticus. However, samples with low scores (<0.850) were classified under other genera. Furthermore, the alterations in FA profiles of V. parahaemolyticus in the presence of various environmental pressures were investigated. The production of saturated FA (SFA) increased gradually concomitant with a decreased proportion of unsaturated FA (UFA) with rising temperature. Similarly, the SFA tended to increase at the expense of UFA with prolonged culture time. In addition, V. parahaemolyticus changed its FA profiles to contain increased short-chained FA to resist an acidic environment, whereas alkaline conditions stimulated high production of long-chained FA. Analysis on FA profile is valuable for the physiological study of V. parahaemolyticus and its rapid identification.

Novel Vibrio spp. Strains Producing Omega-3 Fatty Acids Isolated from Coastal Seawater

“…618 and 414, respectively), which had a similar fatty acid profile under mesophilic conditions (Figure 4). As reported previously for other Vibrio isolates [52][53][54], the major fatty acids of these Vibrio strains were 16:1, 16:0, and 18:1. We found that the relative proportion of these fatty acids was temperature dependent for both Vibrio sp.…”

Transcriptional regulation of acetyl CoA and lipid synthesis by PII protein in Synechococcus PCC 7942

“…In cyanobacteria, fatty acid biosynthesis is an essential and fundamental biochemical pathway which provides raw material for lipid synthesis. Fatty acids determine the organization and function of the cell membrane which is associated with the recognition of cell, specificity of species, and immunity of cell . Fatty acids are synthesized from the TCA cycle intermediate acetyl CoA and the esterification of fatty acids leads to the synthesis of lipids .…”