IntroductionToday, olives are grown in all the countries of the Mediterranean basin, especially in central and southern Spain, Italy, Greece, Turkey, Tunisia and Morocco. The world's total area of olive groves is around 10 million ha [1], of which approximately 98 % is in this Mediterranean region, with Spain standing out for its 24.4 % of the global total. Olives are the most important crop in Extremadura in terms of acreage, with some 269,350 ha, of which 70 % are in the province of Badajoz and 30 % in the province of Cáceres. Of this area, about 92 % corresponds to olives for milling [2]. Extremadura's olive oil production is about 52,600 t/ year, representing 3.36 % of Spain's total olive oil [1]. There are 117 mills.Following the European Union's establishment of a criterion for homogeneous production of olive oil [3], in Extremadura 12 olive-growing zones were defined: (1) Gata-Hurdes, (2) Vera-Ambroz, (3) Ibores, (4) Logrosán-Guadalupe, (5) Montánchez, (6) Rest of Cáceres, (7) Alburquerque, (8) Vegas del Guadiana, (9) Tierra de Barros, (10) La Siberia, (11) La Serena and (12) Jerez-Llerena. Currently in Extremadura there are two Protected Designations of Origin (PDO)-"PDO Gata-Hurdes" in the province of Cáceres, which protects virgin olive oils (VOOs) produced in the olive-growing zones of Gata-Hurdes and La Vera-Ambroz, and "PDO Aceite Monterrubio" in the province of Badajoz, for VOO from the olive-growing zones of La Serena and La Siberia. These PDOs certify the origin, authenticity and quality of the olive oils produced in a specific geographic zone.The most representative cultivars in terms of their percentage of cultivated area of the olive-growing zones studied are "Manzanilla Cacereña" with 95.2 and 90.3 % Abstract The content of sterols and erythrodiol + uvaol was studied in 273 virgin olive oil samples from 40 mills in five olive-growing zones of Extremadura (Spain). An analysis of variance showed significant differences at a confidence level of 95 % between the different zones in each of the main sterols and in the sum of erythrodiol + uvaol. The results of a linear discriminant analysis, considering the different olive-growing areas as categorical dependent variables and the different sterols as independent variables, explained 78.2 % of the variance with the first two discriminant functions. The resulting model correctly classified 86.9 % of the samples analysed. A validation study was conducted to verify the goodness of the discriminant analysis, resulting in 79.3 % of the new samples used for validation being correctly classified. In the graphical representation of the different groups studied considering the proposed model's first two discriminant functions, the centroids of the Sierra Norte de Cáceres, Vegas del Guadiana and Tierra de Barros olive-growing zones were clearly separated, but this was not the case for the other two zones-La Serena and La Siberia.