Fatty acid profiling and multivariate analysis in the genus Leucas reveals its nutritional, pharmaceutical and chemotaxonomic significance

Choudhary, Ashish; Sunojkumar, Purayidathkandy; Mishra, Girish

doi:10.1016/j.phytochem.2017.07.007

Cited by 19 publications

(7 citation statements)

References 60 publications

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“…Conjugated hexadecadienoic acids in total lipid fatty acids were detected using reverse-phase HPLC, following the method of [ 10 ] with slight modifications. A total of 5 mg of free fatty acids (see Section 4.2 ) was redissolved in 1 mL of 0.14% glacial acetic acid in acetonitrile, v / v .…”

Section: Methodsmentioning

confidence: 99%

“…Glutinic acid (HOОС-СН=С=СН-СООН) is a substrate for the synthesis of natural allenes. Laballenic (5R,6-octadecadienoic acid), lamenallenic (5R,6,16E-octadecatrienoic acid), and phlomic (7,8-eicosadienoic acid) acids are abundant in the seeds of various Lamiaceae species [ 9 , 10 ]. Compounds containing allene bonds demonstrated antimicrobial and antiviral activity [ 11 ], implying that FAs with allene bonds could have comparable effects.…”

Section: Introductionmentioning

confidence: 99%

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Identification of Conjugated Dienes of Fatty Acids in Vischeria sp. IPPAS C-70 under Oxidative Stress

Sidorov,

Starikov,

Sinetova

et al. 2024

IJMS

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The microalgae Vischeria sp. IPPAS C-70 produces eicosapentaenoic acid. Several stresses cause the formation of fatty acid peaks that resemble hexadecadienoic acids. We used the integrated technique including TLC, HPLC, and GC–MS to search and determine these fatty acids. Double bond positioning in these fatty acids indicated that they were conjugated dienes and allenes. We identified and described natural nine isomers of C16 polyunsaturated fatty acids, including common methylene-interrupted dienes (Δ6,9-16:2, Δ7,10-16:2, Δ9,12-16:2), and unusual conjugated dienes (Δ6,8-, Δ7,9-, Δ8,10-, Δ9,11-, and Δ10,12-16:2), as well as allenic diene (Δ9,10-16:2). We hypothesize that the formation of conjugated dienes and allenes among fatty acids is the result of oxidative stress caused by H2O2. Hydrogen peroxide also caused an increase in saturated at the expense of unsaturated fatty acids, suggesting inhibition either fatty acid desaturases activities or the corresponding gene expression.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Identification of Conjugated Dienes of Fatty Acids in Vischeria sp. IPPAS C-70 under Oxidative Stress

Sidorov,

Starikov,

Sinetova

et al. 2024

IJMS

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“…Samples were dried using nitrogen gas and dissolved in 50 μl hexane. From the prepared sample, 1 µl was subjected to GC-MS (5977A MSD coupled with 7890B GC series equipped with a 30 m × 0.25 mm × 0.25 mm DB-wax capillary column, Agilent Technologies) with a program described by Choudhary et al (2017) (see Supplementary Information). The experiment was repeated and readings were noted for the replicates (n = 3).…”

Section: Analysis Of Rewardmentioning

confidence: 99%

Interaction between Cymbidium aloifolium and Apis cerana: Incidence of an outlier in modular pollination network of oil flowers

Adit

Koul

Choudhary

et al. 2022

Ecology and Evolution

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So far, oil‐rewarding flowers are known to be pollinated only by oil‐collecting bees, which gather and use lipids for larval feed and nest building. As honeybees do not have oil‐collecting appendages on their legs, they have not been associated with pollination of such flowers. In a predominantly Apis pollinated and food deceptive clade of wild Cymbidiums, we investigated the reproductive strategy of Cymbidium aloifolium, hitherto unknown for its floral oil reward. Our study demonstrates the requisites for establishment of mutualistic interaction between the oil flower and Apis cerana indica, a corbiculate bee. Success in pollination requires learning by honeybees to access the food reward, thereby displaying cognitive ability of the pollinator to access the customized reward. Morphometric matching between orchid flowers and the pollinator, and that between pollinia and stigmatic cavity also appear to be essential in the pollination success. Absence of pollinator competition and prolonged flower‐handling time are suggested to promote floral constancy. The present study highlights the need to explore the spectrum of pollination rewards pursued by honeybees, which may include unconventional composition of floral resources.

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“…This recipe has been passed down through generations among the Kelantanese people, but its mechanism has not been studied or its properties analysed scientifically, hence its absence in modern medicine. Studies on the 10.11113/mjfas.v20n2.3464 Leucas genus, specifically its chemical constituents show to be more than 10 groups of flavonoids and phenolic compounds present, including apigenin and tricin [3,4,[6][7][8]. It can be proposed that these secondary metabolites, whether individually or in synergy, may be responsible for the medicinal properties of this plant, including anthelmintic [2,3,6,9,10].…”

Section: Introductionmentioning

confidence: 99%

Investigation on Optimized Flavonoid Extraction from Leucas zeylanica and Its Anthelmintic Activity

Selahuddeen,

Bouguerra,

Hashim

et al. 2024

Mal. J. Fund. Appl. Sci.

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Extraction has been the primary method of concentrating and obtaining a crude essence of plants, and to an extend fruits and nuts. In modern times, there are many methods of extraction developed from simple maceration to using Soxhlet extractor to microwave assisted extraction and ultrasound assisted extraction. However, the more time saving and energy efficient method for the extraction of plants needs to be investigated. This research explored optimization of ultrasound assisted extraction of flavonoids from Leucas zeylanica and the optimization was done on parameters of sonication time (minutes), water bath temperature (°C), volume of solvent to solid ratio (ml/g) and solvent concentration (%) using response surface methodology (RSM). By measuring the total flavonoid content using aluminium colorimetric method and UV visible spectrophotometry, highest flavonoid yield is achieved with 30.38% increase compared to unoptimized method. This occurred with sonication time of 42 minutes, temperature of 55°C, solvent to solid ratio of 40 ml/g and solvent concentration of 100%. The resulting extract was then tested for its anthelmintic (anti-worm) ability, obtaining 40% efficacy of that of Albendazole.

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Fatty acid profiling and multivariate analysis in the genus Leucas reveals its nutritional, pharmaceutical and chemotaxonomic significance

Cited by 19 publications

References 60 publications

Identification of Conjugated Dienes of Fatty Acids in Vischeria sp. IPPAS C-70 under Oxidative Stress

Identification of Conjugated Dienes of Fatty Acids in Vischeria sp. IPPAS C-70 under Oxidative Stress

Interaction between Cymbidium aloifolium and Apis cerana: Incidence of an outlier in modular pollination network of oil flowers

Investigation on Optimized Flavonoid Extraction from Leucas zeylanica and Its Anthelmintic Activity

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