Fatty Acid Transporters (FABPpm, FAT, FATP) in Human Muscle

Bonen, Arend; Miskovic, Dragana; Kiens, Bente

doi:10.1139/h99-033

Cited by 52 publications

(47 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Many studies, including the present study, failed to detect any correlation between FAT/CD36 or FABP pm mRNA expression and protein levels or with changes in the rate of fatty acid transport (Luiken et al, 2002;Chabowski et al, 2006). Moreover, post-transcriptional processing for FABP pm , and especially FAT/CD36, may be a preferred strategy to alter protein levels (Bonen et al, 1999). Therefore it may be more instructive to examine fatty acid transporter protein levels rather than mRNA expression to study the association of trans-sarcolemmal lipid transport with changing energetic demands (Glatz et al, 2010).…”

Section: Trans-sarcolemmal Lipid Transportcontrasting

confidence: 75%

“…Both training protocols in the present study induced upregulation of FAT/CD36, but not FABP pm , protein levels and both protocols produced FAT/CD36 increases of similar magnitude (46% and 43% increases for cold and exercise training, respectively). Exercise training generally increases both FAT/ CD36 and FABP pm protein levels and sarcolemmal fatty acid transport rates in mammals (Bonen et al, 1999;Bradley et al, 2012). Furthermore, FAT/CD36 knock-out mice also had lower myocyte fatty acid uptake during shivering under cold stress (Putri et al, 2015).…”

Section: Trans-sarcolemmal Lipid Transportmentioning

confidence: 99%

See 1 more Smart Citation

Acute cold and exercise training upregulate similar aspects of fatty acid transport and catabolism in house sparrows, Passer domesticus

Zhang

Carter

Eyster

et al. 2015

Journal of Experimental Biology

View full text Add to dashboard Cite

Summit maximum thermoregulatory metabolic rate (M sum ) and maximum exercise metabolic rate (MMR) both increase in response to acute cold or exercise training in birds. Because lipids are the main fuel supporting both thermogenesis and exercise in birds, adjustments to lipid transport and catabolic capacities may support elevated energy demands from cold and exercise training. To examine a potential mechanistic role for lipid transport and catabolism in organismal cross-training effects (exercise effects on both exercise and thermogenesis, and vice versa), we measured enzyme activities and mRNA and protein expression in pectoralis muscle for several key steps of lipid transport and catabolism pathways in house sparrows (Passer domesticus) during acute exercise and cold training. Both training protocols elevated pectoralis protein levels of fatty acid translocase (FAT/CD36), cytosolic fatty acid-binding protein, and citrate synthase (CS) activity. However, mRNA expression of FAT/CD36 and both mRNA and protein expression of plasma membrane fatty acid-binding protein did not change for either training group. CS activities in supracoracoideus, leg and heart, and carnitine palmitoyl transferase (CPT) and β-hydroxyacyl CoA-dehydrogenase activities in all muscles did not vary significantly with either training protocol. Both M sum and MMR were significantly positively correlated with CPT and CS activities. These data suggest that up-regulation of trans-sarcolemmal and intramyocyte lipid transport capacities and cellular metabolic intensities, along with previously documented increases in body and pectoralis muscle masses and pectoralis myostatin (a muscle growth inhibitor) levels, are common mechanisms underlying the training effects of both exercise and shivering in birds.

show abstract

Section: Trans-sarcolemmal Lipid Transportcontrasting

confidence: 75%

Section: Trans-sarcolemmal Lipid Transportmentioning

confidence: 99%

Acute cold and exercise training upregulate similar aspects of fatty acid transport and catabolism in house sparrows, Passer domesticus

Zhang

Carter

Eyster

et al. 2015

Journal of Experimental Biology

View full text Add to dashboard Cite

show abstract

“…The FAT mRNA was measured using previously described procedures (10,16,38,39). Briefly, total RNA was isolated from tissues using the GIT/CsCl centrifugation method (40), with some modifications.…”

Section: Fatty Acid Transportersmentioning

confidence: 99%

FAT/CD36 expression is not ablated in spontaneously hypertensive rats

Bonen

Han

Tandon

et al. 2009

Journal of Lipid Research

Self Cite

View full text Add to dashboard Cite

There is doubt whether spontaneously hypertensive rats (SHR; North American strain) are null for fatty acid translocase (FAT/CD36). Therefore, we examined whether FAT/CD36 is expressed in heart, muscle, liver and adipose tissue in SHR. Insulin resistance was present in SHR skeletal muscle. We confirmed that SHR expressed aberrant FAT mRNAs in key metabolic tissues; namely, the major 2.9 kb transcript was not expressed, but 3.8 and 5.4 kb transcripts were present. Despite this, FAT/CD36 protein was expressed in all tissues, although there were tissue-specific reductions in FAT/CD36 protein expression and plasmalemmal content, ranging from 26-85%. Fatty acid transport was reduced in adipose tissue (250%) and was increased in liver (147%). Normal rates of fatty acid transport occurred in heart and muscle, possibly due to compensatory upregulation of plasmalemmal fatty acid binding protein (FABPpm) in red (1123%) and white muscle (1110%). In conclusion, SHRs (North American strain) are not a natural FAT/CD36 null model, the North American strain of SHR express FAT/CD36, albeit at reduced levels.-Bonen, A., X-X.

show abstract

“…mRNA abundance of FAT/CD36 and FABPpm was determined as previously described (10). FAT/CD36 and FABPpm protein content was determined in both homogenates and giant sarcolemmal vesicles prepared from heart, skeletal muscle, adipose tissue, and liver ( Fig.…”

Section: Northern and Western Blottingmentioning

confidence: 99%

Changes in fatty acid transport and transporters are related to the severity of insulin deficiency

Luiken

Arumugam

Bell

et al. 2002

American Journal of Physiology-Endocrinology and Metabolism

Self Cite

106

119

View full text Add to dashboard Cite

We have examined the effects of streptozotocin (STZ)-induced diabetes (moderate and severe) on fatty acid transport and fatty acid transporter (FAT/CD36) and plasma membrane-bound fatty acid binding protein (FABPpm) expression, at the mRNA and protein level, as well as their plasmalemmal localization. These studies have shown that, with STZ-induced diabetes, 1) fatty acid transport across the plasma membrane is increased in heart, skeletal muscle, and adipose tissue and is reduced in liver; 2) changes in fatty acid transport are generally not associated with changes in fatty acid transporter mRNAs, except in the heart; 3) increases in fatty acid transport in heart and skeletal muscle occurred with concomitant increases in plasma membrane FAT/CD36, whereas in contrast, the increase and decrease in fatty acid transport in adipose tissue and liver, respectively, were accompanied by concomitant increments and reductions in plasma membrane FABPpm; and finally, 4) the increases in plasma membrane transporters (FAT/CD36 in heart and skeletal muscle; FABPpm in adipose tissue) were attributable to their increased expression, whereas in liver, the reduced plasma membrane FABPpm appeared to be due to its relocation within the cell in the face of slightly increased expression. Taken together, STZ-induced changes in fatty acid uptake demonstrate a complex and tissue-specific pattern, involving different fatty acid transporters in different tissues, in combination with different underlying mechanisms to alter their surface abundance.

show abstract

Fatty Acid Transporters (FABPpm, FAT, FATP) in Human Muscle

Cited by 52 publications

References 0 publications

Acute cold and exercise training upregulate similar aspects of fatty acid transport and catabolism in house sparrows, Passer domesticus

Acute cold and exercise training upregulate similar aspects of fatty acid transport and catabolism in house sparrows, Passer domesticus

FAT/CD36 expression is not ablated in spontaneously hypertensive rats

Changes in fatty acid transport and transporters are related to the severity of insulin deficiency

Contact Info

Product

Resources

About