Forty-two neem ecotypes of India have been found to show a wide
variation in the content of oil,
and their physicochemical characteristics (color, specific gravity,
refractive index, iodine value, acid
value, and saponification value), total fatty acid, fatty acid
composition (oleic, stearic, palmitic,
linoleic, myristic, arachidic, and behenic acids), and the key
meliacins (azadirachtin, nimbin, and
salannin). The azadirachtin content did not correlate with any of
the physicochemical and chemical
parameters, but the nimbin and salannin contents correlated
significantly with each other. The
refractive index (+ve correlation) and iodine value (−ve
correlation) showed weak but significant
correlation with the contents of nimbin and salannin. Insect
growth inhibition of Spodoptera litura
revealed a wide variation in the EC50 of the oils. The
salannin and azadirachtin contents of the
oils correlated the most with bioactivity. The iodine and acid
values correlated weakly but
significantly with bioactivity.
Keywords: Neem; neem oil; physicochemical variation in neem; chemical
variation in neem; neem
bioactivity