Fatty liver and hyperlipidemia in iddm (insulin-dependent diabetes mellitus) of streptozotocin-treated shrews

Ohno, Tamio; Horio, Fumihiko; Tanaka, Shin; Terada, Mamoru; Namikawa, Takao; Kitoh, Junzoh

doi:10.1016/s0024-3205(99)00570-6

Cited by 46 publications

(44 citation statements)

References 15 publications

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“…Compared to control animals, plasma triglyceride and ALT levels were increased by factors of three and four, respectively, in the diabetic animals, while AST remained unchanged (data not shown). These parameters indicate that the diabetic animals had non-alcoholic fatty liver damage, which is consistent with the STZ model [29]. One month after the onset of the disease, we used in vivo spin-trapping techniques with EPR to assess free radical production in the bile of the animals.…”

Section: Resultssupporting

confidence: 66%

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Involvement of inducible nitric oxide synthase in hydroxyl radical-mediated lipid peroxidation in streptozotocin-induced diabetes

Stadler

Bonini

Dallas

et al. 2008

Free Radical Biology and Medicine

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Free radical production is implicated in the pathogenesis of diabetes mellitus, where several pathways and different mechanisms were suggested in the pathophysiology of the complications. In this study, we used electron paramagnetic resonance (EPR) spectroscopy combined with in vivo spin-trapping techniques to investigate the sources and mechanisms of free radical formation in streptozotocininduced diabetic rats. Free radical production was directly detected in the diabetic bile, which correlated with lipid peroxidation in the liver and kidney. EPR spectra showed the trapping of a lipidderived radical. Such radicals were demonstrated to be induced by hydroxyl radical through isotope labeling experiments. Multiple enzymes and metabolic pathways were examined as the potential source of the hydroxyl radicals using specific inhibitors. Neither xanthine oxidase, cytochrome P450s, the Fenton reaction, nor macrophage activation were required for the production of radical adducts. Interestingly, inducible nitric oxide synthase (apparently uncoupled) was identified as the major source of radical generation. The specific iNOS inhibitor 1400W as well as L-arginine pretreatment reduced the EPR signals to baseline levels, implicating peroxynitrite as the source of hydroxyl radical production. Applying immunological techniques, we localized iNOS overexpression in the liver and kidney of diabetic animals, which was closely correlated with the lipid radical generation and 4-hydroxynonenal-adducted protein formation, indicating lipid peroxidation. In addition, protein oxidation to protein free radicals occurred in the diabetic target organs. Taken together, our studies support inducible nitric oxide synthase as a significant source of EPR-detectable reactive intermediates, which leads to lipid peroxidation and may contribute to disease progression as well.

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Section: Resultssupporting

confidence: 66%

“…STZ-induced diabetes is associated with signs of fatty liver [29] and was found to show nephropathic changes and kidney damage as well [30]. We have addressed these questions through the combined use of in vivo EPR methodologies and spin trapping to specifically detect increased free radical production in the diabetic rat liver.…”

Section: Introductionmentioning

confidence: 99%

Involvement of inducible nitric oxide synthase in hydroxyl radical-mediated lipid peroxidation in streptozotocin-induced diabetes

Stadler

Bonini

Dallas

et al. 2008

Free Radical Biology and Medicine

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“…This finding is in agreement with the study of Tian et al 16 in that the body weight could be one of the parameters for the study of the pathophysiology of diabetes mellitus. Similar to the hyperlipidaemia, which is another characteristic of insulin-dependent diabetes [18][19][20] , the total blood triglyceride and total blood cholesterol levels also increased in diabetic rats after a single injection of STZ.…”

Section: Discussionmentioning

confidence: 77%

“…It is not only the hyperglycaemia but also the hyperlipidaemia characteristic that is found in the STZ-induced diabetes animal model. The study of high dose STZ-induced DM in musk shrew showed that high plasma lipids were found in STZ-treated shrews 19 . This high accumulation of triglyceride in blood might suggest the relationship of insulin depletion and the function of lipoprotein lipase 18,19 .…”

Section: Discussionmentioning

confidence: 99%

Anti-hyperglycaemic and anti-hyperlipidaemic effects of black and red rice in streptozotocin-induced diabetic rats

Tantipaiboonwong¹,

Pintha²,

Chaiwangyen³

et al. 2017

ScienceAsia

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Increasing numbers of diabetic patients, especially those with type II, can link their condition to changes in eating habits. Black rice and red rice are not only a source of carbohydrate but also rich sources of antioxidants, especially proanthocyanidins and anthocyanins. This study determines the effect consuming a black rice extract (BRE) or a red rice extract (RRE) on streptozotocin-induced diabetic rats. Blood levels of glucose, triglycerides, and cholesterol in the diabetic group were determined and compared to that of the control group. The results demonstrate that consumption of BRE, 50 mg/kg body weight (BW), or RRE, 100 mg/kg BW, could significantly reduce the blood glucose level of the diabetic rats after eight weeks. Consumption of BRE, 100 mg/kg BW or RRE, 50 mg/kg BW, also clearly decreased the triglyceride level. The cholesterol level in the diabetic group fed with BRE or RRE decreased as well. Thus daily consumption of black rice, red rice, or having their extracts in food supplements may prevent the onset of diabetes.

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“…Since insulin has a potent inhibitory effect on lipolysis in adipocytes, insulin deficiency is associated with excess lipolysis and increased influx of free fatty acids to the liver [38][39][40][41]. The increased levels of LDL and VLDL in the diabetic animals might be due to over production of LDL and VLDL by the liver due to the stimulation of hepatic triglyceride synthesis as a result of free fatty acid influx [39].…”

Section: Dissuasionmentioning

confidence: 99%

Novel Anti-Diabetic Formula: Recover Islet ?-Cell dysfunction with the implementation of molecular approach

Kosta

Dangi

Shekhar

et al. 2015

Int J of Adv in Sci Res

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The regulation of PPARα and PPARγ at m-RNAs expression level is responsible for Islet β-cell dysfunction and insulin secretion seen in diabetic rats. Many traditional treatments have been recommended in the alternative system of medicine for the treatment of diabetes mellitus. Stevia rebaudiana and Gurmmar is a nontoxic natural therapeutic agent revealed to own diuretic, hypotensive and hypoglycemic properties. The ground of this study was to examine the anti-hyperglycemic property, expression of m-RNA at PPARα and PPARγ receptors and islet β-cells dysfunction of a combined aqueous extracts of Stevia rebaudiana and Gurmmar is on streptozotocin induced diabetic wistar rats. The diabetes induced rats were fed with plant extracts at the increasing dosage of 200mg, 300mg and 500mg of body weight. The combined plant extracts administrated rats revealed a significantly (P<0.001) rise of serum insulin levels and higher reduction in hyperglycemia or hyperlipidemia when compared to the diabetic control rats (P<0.001) and also an increase the m-RNAs expansion of PPARα and PPARγ respectively. The histological studies of the endocrine region of pancreas of diabetic rats revealed that shrinkage of β-cells of islets of langerhans. The combined plant extracts treated rats revealed recovery of β-cells. The recovery of β-cells was evident at higher dose level i.e. 500mg/wt extracts fed groups. According to the biochemical, molecular and histological results obtained, it was concluded that the combined plant extracts of this plant may sustain anti-diabetic properties by recovery Islet β-cell dysfunction with the implementation of molecular approach.

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Fatty liver and hyperlipidemia in iddm (insulin-dependent diabetes mellitus) of streptozotocin-treated shrews

Cited by 46 publications

References 15 publications

Involvement of inducible nitric oxide synthase in hydroxyl radical-mediated lipid peroxidation in streptozotocin-induced diabetes

Involvement of inducible nitric oxide synthase in hydroxyl radical-mediated lipid peroxidation in streptozotocin-induced diabetes

Anti-hyperglycaemic and anti-hyperlipidaemic effects of black and red rice in streptozotocin-induced diabetic rats

Novel Anti-Diabetic Formula: Recover Islet ?-Cell dysfunction with the implementation of molecular approach

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