Fc Receptors for IgG(1) and IgG(3) on Human Mononuclear Cells -
An Evaluation with Known Levels of Erythrocyte-Bound IgG

Zupańska, B; Thomson, Eileen E.; Merry, Anthony H

doi:10.1159/000461399

Cited by 16 publications

(22 citation statements)

References 8 publications

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“…The minimum levels of sensitization required for rosette formation with monocytes previously found for polyclonal anti-D antibodies [8] were 180-460 IgG mol ecules per red cell for IgG3-E and 1,180-4,300 IgG mol ecules per red cell for IgGl-E. Values obtained with the monoclonal antibodies now tested were very similar and probably within experimental error for the assays.…”

Section: Discussionsupporting

confidence: 68%

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Ability of Monoclonal Anti-D Antibodies to Promote the Binding of Red Cells to Lymphocytes, Granulocytes and Monocytes

Merry¹,

Brojer²,

Zupanska³

et al. 1989

Vox Sanguinis

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Red cells sensitized with IgGl or IgG3 monoclonal anti-D antibodies were used in rosette assays with human lymphocytes, monocytes and granulocytes. With all three cell types, IgG3 antibodies promoted a greater degree of rosette formation than IgGl antibodies. Monocytes required a minimum of about 0.5 x 10^3 IgG3 molecules per red cell for rosette formation, and granulocytes and lymphocytes required around 1 x 10^4 IgG3 molecules per red cell. Approximately 80% of monocytes and granulocytes and 10% of lymphocytes were capable of rosette formation. These results are consistent with differences in the number and affinity of Fc receptors on different leucocytes. When compared with previous data these results suggest that binding of monocytes to monoclonal anti-D sensitized red cells is very similar to that of red cells sensitized with polyclonal antisera. Lymphocytes and granulocytes, however, appear to bind less well to red cells sensitized with certain monoclonal antibodies than with polyclonal antibodies. These findings may be of relevance to the prophylactic use of monoclonal anti-D antibodies.

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Section: Discussionsupporting

confidence: 68%

“…This suspension contained greater than 90% of monocytes as judged by morphological standards (May-Grtinwald-Giesma staining and staining for esterase and peroxidase). The rosette assay was performed as previously described [8], the criteria for rosettes being 3 or more red cells attached per leucocyte.…”

Section: Separation Of Monocytes and Lymphocytesmentioning

confidence: 99%

Ability of Monoclonal Anti-D Antibodies to Promote the Binding of Red Cells to Lymphocytes, Granulocytes and Monocytes

Merry¹,

Brojer²,

Zupanska³

et al. 1989

Vox Sanguinis

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“…Capacity of IgG antibodies to bind Fc receptors on monocytes is greater than that of IgGl antibodies. It has been demonstrated that for interaction between red cells and monocytes 4-5 times more molecules of IgGl than IgG3 are required [17,18]. Therefore Rh HDN is expected to be more severe when anti-D is of IgG3 type.…”

Section: Discussionmentioning

confidence: 99%

Distribution of IgG Subtypes in Maternal Anti-D Sera and Their Prognostic Value in Rh Haemolytic Disease of the New-Born

Iyer¹,

Kulkarni²,

Gupte³

1992

Acta Haematol

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In 107 Rh(D)-immunized women having Rh(D)-positive pregnancy screening for IgG subtypes was carried out between the 34th and 36th week of gestation. Using polyclonal IgG subtype-specific reagents, all four IgG subclasses were detected in anti-D sera though IgGl and IgG3 were the most predominant classes. IgG3 anti-D was usually low titre. At the same level of Rh(D) antibody titre, haemolytic disease of the new-born was more severe when anti-D was IgGl type than IgG3 type. When the IgGl and IgG3 anti-D subtypes existed together, the risk of having a stillborn child was very high compared to other subtypes (IgG1 + IgG3 34.8%, IgG1 19.2% and IgG3 15.4%).

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“…Under these conditions, IgA-, IgG2-and IgG4-coated RBCs were found not to react with mononuclear phagocytes and it became com mon practice to state that mononuclear phagocytes had receptors for IgGl [101] found that they needed 500 IgGl, but only 100 IgG3 mole cules per RBC, to obtain phagocytosis in their in vitro monocyte assay. Zupanska et al [ 102] found that the minimum number of molecules per RBC needed for monocyte adherence was 180-460 IgG3 and 1,180-4,300 IgG 1 molecules. In a later paper [ 103], they showed that the maximum number of …”

Section: Qualitative Differences Versus Quantitative Differencesmentioning

confidence: 99%

Effect of Cell-Bound Proteins on the in vivo Survival of Circulating Blood Cells

Garratty¹

1991

Gerontology

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Normal circulating red blood cells (RBCs) and platelets have been shown to have small amounts of IgG on their membranes. The cell-bound IgG may be cytophilic (IgG nonspecifically adsorbed from the plasma) and/or IgG autoantibody. It has been suggested that most of the RBC-bound IgG is on older RBCs and is an autoantibody directed against senescent cell antigen (SCA). The accumulation of this RBC-bound IgG leads to Fc-dependent removal of senescent RBCs by macrophages in the reticuloendothelial system. RBCs also have complement components on their membrane; it is not clear how this accumulates on RBCs and whether it has a physiologic function. This small amount of nonpathogenic RBC-bound IgG is not detected by the antiglobulin test. It is still unclear whether the major difference between pathogenic and nonpathogenic IgG autoantibodies is qualitative, quantitative, or both. Seemingly healthy blood donors (1 in 1,000 donors) and patients without hemolytic anemia (up to 8% of patients) have increased amounts ( > 200 molecules/RBC) of RBC-bound IgG and complement that is detected by the antiglobulin test. This RBC-bound IgG has been shown to be an IgG autoantibody directed against blood group antigens, and/or IgG anti-idiotype, and/or IgG nonspecifically adsorbed onto the RBC membrane when plasma IgG levels are high. Most patients with autoimmune hemolytic anemia (AIHA) have RBC-bound IgG and/or complement detectable by the antiglobulin test. Most of the RBC-bound IgG is of the IgG1 subclass, whether one examines the RBCs of healthy blood donors or hospitalized patients with and without AIHA. Although the quantity of RBC-bound IgG is generally higher in patients with AIHA, there is no clear correlation with the quantity of RBC-bound IgG and the rate of in vivo RBC destruction. There is some recent evidence that the SCA autoantibody may at times be pathogenic and cause autoimmune disease.

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Fc Receptors for IgG(1) and IgG(3) on Human Mononuclear Cells - An Evaluation with Known Levels of Erythrocyte-Bound IgG

Cited by 16 publications

References 8 publications

Ability of Monoclonal Anti-D Antibodies to Promote the Binding of Red Cells to Lymphocytes, Granulocytes and Monocytes

Ability of Monoclonal Anti-D Antibodies to Promote the Binding of Red Cells to Lymphocytes, Granulocytes and Monocytes

Distribution of IgG Subtypes in Maternal Anti-D Sera and Their Prognostic Value in Rh Haemolytic Disease of the New-Born

Effect of Cell-Bound Proteins on the in vivo Survival of Circulating Blood Cells

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