FcRIII (CD16) expression on neutrophils from chronic myeloid leukemia. A flow cytometric study

Carulli, Giovanni; Gianfaldoni, Maria Luisa; Azzarà, Antonio; Papineschi, Federico; Vanacore, Renato; Minnucci, S; Testi, Rossana; Ambrogi, F

doi:10.1016/0145-2126(92)90120-v

Cited by 25 publications

(14 citation statements)

References 27 publications

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“…PMN separation. PBMC were separated from plasma by Lymphoprep (Nyegaard & Co., Oslo, Norway) density gradient centrifugation by the method of Boyum (5) and PMN were obtained as previously described (9). The cell viability, checked by trypan blue exclusion, was always greater than 99%.…”

Section: Methodsmentioning

confidence: 99%

Modulation of Human Immune Response byEchinococcus granulosusAntigen B and Its Possible Role in Evading Host Defenses

Riganò

Profumo

Bruschi³

et al. 2001

Infect Immun

143

117

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). AgB skewed the Th1/Th2 cytokine ratios towards a preferentially immunopathology-associated Th2 polarization, predominantly in patients with progressive disease. AgB-stimulated patients' PBMC also proliferated less than SHF-stimulated PBMC (P ‫؍‬ 9 ؋ 10 ؊3 ). In vitro Th2 cytokine production was reflected in vivo by elevated specific immunoglobulin E (IgE) and IgG4 antibodies binding to AgB. These findings confirm that AgB plays a role in the escape from early immunity by inhibiting PMN chemotaxis. They also add new information on the host-parasite relationship, suggesting that AgB exploits the activation of T helper cells by eliciting a nonprotective Th2 cell response.Cystic echinococcosis (CE) is a widely endemic helminthic disease caused by infection with metacestodes (larval stage) of the tapeworm Echinococcus granulosus. It affects humans and a wide range of livestock species (28). The disease is characterized by the growth in the host internal organs, mostly liver and lungs, of steadily growing fluid-filled, unilocular cysts surrounded by a two-layered hydatid cyst wall. The main feature of the host-parasite relationship is the coexistence of the chronic infection with detectable humoral and cellular responses against the parasite. Parasite survival in vivo depends on efficient evasion mechanisms starting to operate as the parasite develops toward a hydatid cyst. A fibrotic host capsule of variable thickness usually develops, thus forming together with the parasite-derived acellular laminated layer a formidable cystic structure. As a consequence, the actively dividing germinal layer within the cyst along with its associated brood capsule and enclosed protoscoleces are effectively sequestered from the host immune responses. In addition to this physical barrier, the hydatid cyst has probably evolved other strategies for immune evasion. Although older models suggested a more passive role for parasites in immune evasion-for example sequestration, antigenic masking by host proteins, and global immunosuppression-later studies suggest in human parasitoses the active deployment of strategies that manipulate and exploit the host response (12, 30).

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Section: Methodsmentioning

confidence: 99%

Modulation of Human Immune Response byEchinococcus granulosusAntigen B and Its Possible Role in Evading Host Defenses

Riganò

Profumo

Bruschi³

et al. 2001

Infect Immun

143

117

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“…Superoxide anion production by neutrophils was evaluated as de scribed previously [16], Formyl-methionyl-leucyl-phenylalaninc (FMLP: Sigma Chemical) was used as stimulating agent at a final con centration of 10 " M. Values were expressed as nanomoles/10'1 neutrophils/10 min.…”

Section: Superoxide Anion Productionmentioning

confidence: 99%

Platelet-Neutrophil Interactions in Uremic Patients: Effects on Neutrophil Superoxide Anion Production and Chemiluminescence

Carulli

Barsotti²,

Cupisti³

et al. 1995

Nephron

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Isolated resting platelets are able to limit neutrophil activation and then can control the tissue-damaging potential of activated neutrophils. In the present study, platelet-neutrophil interactions have been evaluated in 10 uremic patients; the blood samples have been collected before the hemodialysis session. Twelve normal subjects served as controls. Platelets and neutrophils have been isolated and recombined in an autologous ex vivo system. Anion superoxide production and chemiluminescence (which is related to hypochlorous acid production) have been evaluated after stimulation with N-formyl-methionyl-leucyl-phenylalanine. Coincubation of platelets from normal subjects with autologous neutrophils led to a dose-dependent inhibition of both superoxide anion generation induced by N-formyl-methionyl-leucyl-phenylalanine and chemiluminescence. Instead, platelets from uremic patients have not affected superoxide anion production by autologous neutrophils. The chemiluminescence was reduced by coincubation with autologous platelets only at the highest platelet-neutrophil ratio (100:1). In conclusion, the modulation exerted by platelets towards neutrophil activation can be impaired in chronic uremia. Therefore, the tissue-damaging potential of circulating neutrophils, due to toxicity by superoxide anion and hypochlorous acid, may be increased.

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“…Leukocytes were separated according to Boyum [17]and neutrophil polymorphonuclear cells were separated as previously described [18]. Cell viability, checked by trypan blue exclusion, was always greater than 90%.…”

Section: Methodsmentioning

confidence: 99%

Inhibitory Effects of Human Neutrophil Functions by the 45-kD Glycoprotein Derived from the Parasitic Nematode <i>Trichinella spiralis</i>

Bruschi

Carulli

Azzarà

et al. 2000

Int Arch Allergy Immunol

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Aim: We evaluated the effect of the 45-kD protein of Trichinella spiralis (gp45), purified by affinity chromatography, on random migration and chemotaxis, the oxidative metabolism of human neutrophils and on the CD11b upregulation induced by formyl-methionyl-leucyl-phenylalanine (f-MLP). Methods: Donor neutrophils incubated with different amounts of gp45 (0.5, 1, 1.5, 2 μg/ml) or buffer and the random migration and chemotaxis, evaluated by means of a special technique of image analysis, and the chemiluminescence response to f-MLP or phorbol myristate acetate (PMA) were analyzed. The effect on CD11b upregulation was assessed incubating cells with the protein, when activating them with f-MLP. Results: The results showed that gp45 inhibited both random and stimulated migrations, and reduced the response to f-MLP and PMA. Furthermore, gp45 significantly reduced the upregulation of the CD11b induced by f-MLP. Conclusion: The results show that gp45 inhibits PMN in different functions, suggesting an anti-inflammatory action.

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FcRIII (CD16) expression on neutrophils from chronic myeloid leukemia. A flow cytometric study

Cited by 25 publications

References 27 publications

Modulation of Human Immune Response byEchinococcus granulosusAntigen B and Its Possible Role in Evading Host Defenses

Modulation of Human Immune Response byEchinococcus granulosusAntigen B and Its Possible Role in Evading Host Defenses

Platelet-Neutrophil Interactions in Uremic Patients: Effects on Neutrophil Superoxide Anion Production and Chemiluminescence

Inhibitory Effects of Human Neutrophil Functions by the 45-kD Glycoprotein Derived from the Parasitic Nematode <i>Trichinella spiralis</i>

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