Fe deficiency induced changes in rice (Oryza sativa L.) thylakoids

Abstract

Iron deficiency is an important abiotic stress that limits productivity of crops all over the world. We selected a hybrid rice (Oryza sativa L.), LYPJ, which is super high-yield and widely cultured in China, to investigate changes in the components and structure of thylakoid membranes and photosynthetic performance in response to iron deficiency. Our results demonstrated that photosystem I (PSI) is the primary target for iron deficiency, while the changes in photosystem II (PSII) are important for rebuilding a balance in disrupted energy utilization and dissipation caused by differential degradation of photosynthetic components. The result of immunoblot analysis suggested that the core subunit PsaA declined drastically, while PsbA remained relatively stable. Furthermore, several organizational changes of the photosynthetic apparatus were found by BN-PAGE, including a marked decrease in the PSI core complexes, the Cytb /f complex, and the trimeric form of the LHCII antenna, consistent with the observed unstacking grana. The fluorescence induction analysis indicated a descending PSII activity with energy dissipation enhanced markedly. In addition, we proposed that the crippled CO assimilation could be compensated by the enhanced of phosphoenolpyruvate carboxylase (PEPC), which is suggested by the decreased ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) and photosynthetic efficiency.

Comparative Transcriptomics and Proteomics of Atractylodes lancea in Response to Endophytic Fungus Gilmaniella sp. AL12 Reveals Regulation in Plant Metabolism

“…Plantlets were harvested at 10: 00 AM at 15 DPI for Chlorophyll a fluorescence measurement using a Handy-PEA instrument (Handy PEA, Hansatech, United Kingdom) (Wang et al, 2017). Prior to measurement, A. lances leaves were dark-adapted for 30 min at room temperature to relax the reaction centers.…”

Nitrogen fertilization coupled with iron foliar application improves the photosynthetic characteristics, photosynthetic nitrogen use efficiency, and the related enzymes of maize crops under different planting patterns

“…However, iron, which is a micronutrient is often required in small quantities, though it plays a major role in photosynthetic electron transport. It is one of the most important elements involved in chlorophyll formation, photosynthesis, and the photosynthetic enzyme (i.e., ribulose 1,5-bisphosphate carboxylase/oxygenase) in the plant (Wang et al, 2017;Yoon et al, 2019;Maitra et al, 2022). Iron also plays a pivotal role in nitrogen assimilation, uptake, and translocation by regulating the cofactor enzymes of nitrogen metabolism [i.e., nitrate reductase (NR), nitrite reductase (NiR), and glutamate synthase (GOGAT)] (Borlotti et al, 2012).…”

Seed Priming with Iron Oxide Nanoparticles Modulate Antioxidant Potential and Defense-Linked Hormones in Watermelon Seedlings

“…In plants, Fe is essential to perform various cellular processes, such as respiration and photosynthesis. It also serves as a cofactor for enzymes such as cytochrome P450s and Fe­(II)/2-oxoglutarate-dependent oxygenases and helps to remove a kinetic barrier of enzymatic reactions, which involves electron-transfer or oxygen-transfer processes. , Gibberellic acid (GA), jasmonic acid (JA), and salicylic acid (SA) have critical roles in Fe homeostasis in plants. , Recent studies demonstrated that Fe provides added immunity to plants against biotic stresses, and its deficiency causes abiotic stress. , The effects of Fe deficiency on the major plant defense hormones such as SA and JA have been more clearly understood in recent years . It has been found that Fe deficiency enhances SA accumulation in Arabidopsis .…”

“…5,8 Recent studies demonstrated that Fe provides added immunity to plants against biotic stresses, and its deficiency causes abiotic stress. 9,10 The effects of Fe deficiency on the major plant defense hormones such as SA and JA have been more clearly understood in recent years. 5 It has been found that Fe deficiency enhances SA accumulation in Arabidopsis.…”