FE-SEM observation, and mechanism of interaction of wet agar gel in various swelling conditions using hydrophilic ionic liquid

Takahashi, Chisato; Shirai, Takashi; Fuji, Masayoshi

doi:10.1016/j.matchemphys.2012.08.004

Cited by 9 publications

(6 citation statements)

References 44 publications

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“…From water content results (Table 1), there is small amount of water still remained within HAp porous green body after IL treatment in desiccator for 2 h + vacuum condition for 24 h. Recently, we reported that the formation of weak hydrogen bond between water and IL was not completely dissociated during heating and vacuum condition. 31 In the similar mechanism, it can be considered that weak hydrogen bonding exist within HAp porous green body subjected to IL treatment with the remained water molecules.…”

Section: Conditionmentioning

confidence: 99%

See 1 more Smart Citation

A simple approach to observe non-conductive hydrated materials with FE-SEM: Case study on porous hydroxyapatite green bodies

Takahashi

Pattanayak

Shirai

et al. 2013

Journal of the European Ceramic Society

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Section: Conditionmentioning

confidence: 99%

“…Most recently, present authors also proposed that agar gel which is a hydrated material can be observed in FE-SEM when it is previously treated with the IL solution. [29][30][31] Although some reports suggest the suitability of IL for microscopic observation, still this method is limited to few kinds of materials.…”

Section: Introductionmentioning

confidence: 99%

A simple approach to observe non-conductive hydrated materials with FE-SEM: Case study on porous hydroxyapatite green bodies

Takahashi

Pattanayak

Shirai

et al. 2013

Journal of the European Ceramic Society

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“…To solve these problems, simple observation techniques, such as an ionic liquid (IL) and nano‐suit have recently been reported. We also developed novel SEM observation techniques using hydrophilic IL in accordance with the interaction between hydrophilic IL and water, and performed high‐resolution imaging of biological materials . Room‐temperature ILs are organic salts that exist as fluids at room temperature .…”

Section: Introductionmentioning

confidence: 99%

SEM imaging of the stimulatory response of RAW264.7 cells against Porphyromonas gingivalis using a simple technique employing new conductive materials

et al. 2017

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In the medical biology, it is essential to understand not only biological morphology but also the interaction between biological materials and agents. To study these, electron microscopy (EM) is often utilized. However, sample preparation techniques for EM require a high level of skill and a considerable time. Here, we conducted EM using a simple technique employing a conductive liquid, BEL-1, and compared the results with another simple technique employing an ionic liquid, 1-butyl-3-methylimidazolium tetrafluoroborate ([BMIM][BF ]). BEL-1 was used for sample pretreatment, and the morphologies of the mouse RAW 264.7 cell line, Porphyromonas gingivalis, and the RAW 264.7 cell line were stimulated via co-incubation with P. gingivalis and observed using field emission scanning EM (FE-SEM). In the present study, the inflammation-induced system of P. gingivalis was successfully established. FE-SEM results revealed the fine morphology of the RAW 264.7 cell line and P. gingivalis and confirmed a morphological change in the RAW 264.7 cell line caused by P. gingivalis stimulation. Using the developed sample preparation technique employing BEL-1, high-contrast and high-resolution observations of deformable biological materials were conducted without any difficulty or the necessity for complicated technique. This morphological information and the developed techniques can contribute to reveal the interaction between biological materials and agents and thereby accelerate drug formulation and disease treatment. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 1280-1285, 2018.

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“…IL in the SEM was first used for in‐situ characterization of metal deposition on electrodes plunged in 1‐butyl‐3‐methylimidazolium bis(trifluoromethanesulfonyl)imide as the electrolyte (Arimoto, ) as long as electrodes aging in working conditions (Arimoto et al, ). Takahashi et al reported the use of IL in agar gels (Takahashi et al, ) and wet porous hydroxyapatite green bodies specimens (Takahashi et al, ) prior to SEM observation. He reported the ease of sample preparation, the reducing of charging effects during electron beam irradiation of the sample and negligible shrinkage of the original structure of the specimens.…”

Section: Introductionmentioning

confidence: 99%

“…Exploring the limits of the technique, Dwiranti et al () were able to image human chromosomes at high resolution without dehydration and metal coating. In water‐based specimens' applications, a hydrophilic IL must be used to partially exchange with the water contained in the specimens and generate a network with the remaining water inside the specimen preventing shrinkage when exposed to high vacuum (Takahashi et al, ).…”

Section: Introductionmentioning

confidence: 99%

Ionic liquid-based observation technique for nonconductive materials in the scanning electron microscope: Application to the characterization of a rare earth ore

Brodusch

Waters

Demers

et al. 2014

Microsc. Res. Tech.

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A new approach for preparing geological materials is proposed to reduce charging during their characterization in a scanning electron microscope. This technique was applied to a sample of the Nechalacho rare earth deposit, which contains a significant amount of the minerals fergusonite and zircon. Instead of covering the specimen surface with a conductive coating, the sample was immersed in a dilute solution of ionic liquid and then air dried prior to SEM analysis. Imaging at a wide range of accelerating voltages was then possible without evidence of charging when using the in-chamber secondary and backscattered electrons detectors, even at 1 kV. High resolution x-ray and electron backscatter diffraction mapping were successfully obtained at 20 and 5 kV with negligible image drifting and permitted the characterization of the microstructure of the zircon/fergusonite-Y aggregates encased in the matrix minerals. Because of the absence of a conductive layer at the surface of the specimen, the Kikuchi band contrast was improved and the backscatter electron signal increased at both 5 and 20 kV as confirmed by Monte Carlo modeling. These major developments led to an improvement of the spatial resolution and efficiency of the above characterization techniques applied to the rare earth ore and it is expected that they can be applied to other types of ores and minerals.

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FE-SEM observation, and mechanism of interaction of wet agar gel in various swelling conditions using hydrophilic ionic liquid

Cited by 9 publications

References 44 publications

A simple approach to observe non-conductive hydrated materials with FE-SEM: Case study on porous hydroxyapatite green bodies

A simple approach to observe non-conductive hydrated materials with FE-SEM: Case study on porous hydroxyapatite green bodies

SEM imaging of the stimulatory response of RAW264.7 cells against Porphyromonas gingivalis using a simple technique employing new conductive materials

Ionic liquid-based observation technique for nonconductive materials in the scanning electron microscope: Application to the characterization of a rare earth ore

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