Fe₃O₄ @ PLGA‐PEG Nanocomposite for Improved Delivery of Methotrexate in Cancer Treatment

Abstract

Magnetic Fe 3 O 4 nanoparticles are gaining significance in drug delivery applications owing to their targeting capability. Surface modification of amphiphilic block polymers by Fe 3 O 4 nanoparticles increases their properties. In this study, Fe 3 O 4 @ PLGA-PEG nanocomposite is prepared by double emulsion (w/ o/w) method. A shift in the 2Θ values for the composite in XRD attributes to interaction between Fe 3 O 4 and PLGA-PEG. Also, a shift in the Fe-O band in the FTIR spectrum of Fe 3 O 4 @PLGA-PEG from 578 cm -1 to 510 cm -1 confirms the formation of nanocomposite. Surface morphology of the prepared nanocomposite is analyzed by TEM and AFM. Decrease in agglomeration due to electrostatic repulsion between the polymer chains and magnetic particles is observed while an increased surface area (61.0nm) confirms the formation of the nanocomposite. To determine the effectiveness of the prepared magnetically modified nanoparticles, methotrexate (anticancer drug) is encapsulated into the nanocomposite. High entrapment effi-ciency of 95% is observed when polymer:drug is 1:1. The invitro release profile shows that pH of release medium plays a significant role. At physiological pH of 7.3 there is only 15% methotrexate release while nearly 86% of methotrexate release is observed at acidic pH of 4.6 over 72h. Korsemeyer-Peppas model of drug release (R 2 -0.9868) represents swelling controlled release of methotrexate. Further, the cytotoxic cell viability assay on SK-BR-3 (breast adinocarcinoma) cells showed that methotrexate loaded onto the nanocomposite showed higher cell viability as compared to free methotrexate after 96h of incubation. The fluorescent cell imaging also showed that methotrexate released slowly from the nanoparticles and diffused into the nucleus without losing its cytotoxic effect on the cancer cells. Based on these properties of the magnetically modified PLGA-PEG nanoparticles they can be used as targeting drug delivery agents in treatment of cancer therapy.

Anticancer, Antibacterial and Hyperthermia Studies of a Caffeine‐Based N‐Heterocyclic Carbene Silver Complex Anchored on Magnetic Nanoparticles

“…Recently, Fe 3 O 4 magnetic nanoparticles (MNPs) have emerged as intriguing class of advanced materials that possess unique features such as biocompatibility, chemical inertness, relatively less toxicity, high stability, superparamagnetism, [7] facile surface functionalization [8] and easy magnetic retrievability [9] . In this context, Fe 3 O 4 MNPs have been studied extensively for diverse biomedical applications [10] such as diagnostic imaging, drug delivery, [11] magnetic separation, [12] hyperthermia [13,14] and cancer treatment [15] . Fe 3 O 4 MNPs have received special attention in cancer treatments using magnetic hyperthermia for their great potential as mediators of heat for localized hyperthermia therapy.…”

X-ray-Triggered CO Release Based on GdW10/MnBr(CO)5 Nanomicelles for Synergistic Radiotherapy and Gas Therapy

“…GW/MnCO@PLGA nanomicelles with excellent biocompatibility were prepared by oil-in-water (O/W) emulsification. − Nanomicelles could release CO under X-ray irradiation and cooperate with radiotherapy and gas therapy to achieve a good tumor inhibition effect (Scheme ). GdW 10 NPs were synthesized via a previously reported method .…”

Development of L-carnosine functionalized iron oxide nanoparticles loaded with dexamethasone for simultaneous therapeutic potential of blood brain barrier crossing and ischemic stroke treatment

“…Surface morphology observation through AFM and SEM techniques: (a, a1) Fe 3 O 4 nanoparticles, (b, b1) MNP composited particles and (c, c1) dm@LMNP nanoformulations. Basu et al, 2018). A peak at 1632 cm À1 is corresponding to the bending vibration of O-H adsorbed on the Fe 3 O 4 surface which is shown in Figure 3(Ba).…”