Fe³⁺/⁻ dual doped nano hydroxyapatite: A novel material for biomedical applications

Abstract

Dual ions substituted hydroxyapatite (HA) received attention from scientists and researchers in the biomedical field owing to their excellent biological properties. This paper presents a novel biomaterial, which holds potential for bone tissue applications. Herein, we have successfully incorporated ferric (Fe 31 )/selenate (SeO 22 4 ) ions into the HA structure (Ca 10-x-y Fe y (PO 4 ) 6-x (SeO 4 ) x (OH) 2-x-y O y ) (Fe-SeHA) through a microwave refluxing process. The Fe-SeHA materials were characterized by X-ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy, and field emission scanning electron microscopy (FESEM). XRD and FTIR analyses revealed that Fe-SeHA samples were phase pure at 9008C. FESEM images showed that formation of rod-like shaped particles was inhibited dramatically with increasing Fe 31 amount. The Vickers hardness (HV) test showed that hardness values increased with increasing Fe 31 concentrations. Optical spectra of Fe-SeHA materials contained broadband over (200-600) nm. In vitro degradation and bioactivity tests were conducted in simulated body fluid (SBF). The incorporation of Fe 31 /SeO 22 4 ions into the HA structure resulted in a remarkably higher degradation rate along with intense growth of apatite granules on the surface of the Fe-SeHA discs with Ca/P ratio of 1.35-1.47. In vitro protein adsorption assay was conducted in fetal bovine serum (FBS) and it was observed that the adsorption of serum proteins on Fe-SeHA samples significantly increased with increasing Fe 31 concentration. In vitro cytotoxicity tests were performed with human fetal osteoblast (hFOB)

Use of Nanoparticles in Tissue Engineering and Regenerative Medicine

“…Application of co-doped HAp NPs in bone tissue engineering has focused to enhance cellular activity including attachment, proliferation and differentiation. Alshemary et al (2017) have successfully synthesized ferric (Fe 3+ )/selenite (Se42-) co-doped HAp materials via microwave refluxing procedure. Degree of crystallinity was decreased and crystallite growth was inhibited by increasing the amount of Fe 3+ doping.…”

Selenium-Doped Hydroxyapatite Nanocrystals–Synthesis, Physicochemical Properties and Biological Significance

“…These promising results obtained for hydroxyapatite materials containing selenium mean that the subject is still relevant. It is possible to find some attempts in the literature to synthesize co-substituted hydroxyapatites, e.g., those containing selenium IV ions along with Mn 2+ manganese, Zn 2+ zinc or Fe 3+ iron ions [59,70,71]. There have also been attempts to use Se-HA to create systems for the delivery of medicinal substances to bone tissue, e.g., antiretroviral drugs (from the group of bisphosphonates) or antibiotics [36].…”

Silicate-doped nano-hydroxyapatite/graphene oxide composite reinforced fibrous scaffolds for bone tissue engineering

“…The peaks present at 1047 and 1099 cm −1 are related to vibrations of C–O bond. 21 HA and SiHA spectra showed that a small but broad band related to the P–O–P vibration originates from PO 4 3– group located at 570 cm −1 , 22 and the weakness in intensity was due to the overlapping with PCL specific absorptions. Oxygen-containing functional groups of GO particles have characteristic absorption peaks at 1720 cm −1 due to the stretching vibration of C = O, 1620 cm −1 from the C = C stretching, 1400 cm −1 from –OH groups and 1300–1350 cm −1 from the C–OH stretching vibrations.…”