Fe2O3/Chitosan coated superparamagnetic nanoparticles supporting lipase enzyme from Candida Antarctica for microwave assisted biodiesel production

Ariffin, Maryam Farhana Kamel; Idris, Ani

doi:10.1016/j.renene.2021.11.077

Cited by 33 publications

(13 citation statements)

References 60 publications

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“…Furthermore, either IPM or IPP conversion exhibits a decreasing slope in the increase of reaction time. This may be attributed to the decreasing enzyme lipase activity due to increasing reaction temperature in the prolonged reaction time . The reaction temperature was observed to have increased from room temperature to 50 °C after 30 min of reaction time.…”

Section: Resultsmentioning

confidence: 98%

“…However, solid acid catalysts also have some drawbacks, such as low thermal stability, low surface area, and solubility in polar media reducing catalytic activity . Lipase enzymes have also been used as catalysts to replace these acid catalysts and have been shown to catalyze esterification reactions with high yields. , However, the enzymatic reaction requires a long reaction time to complete and, hence, is insufficient …”

Section: Introductionmentioning

confidence: 99%

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Microwave-Assisted Enzymatic Esterification Production of Emollient Esters

Sitepu,

Hudiyono,

Sinaga

et al. 2023

ACS Omega

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Currently, esterification production of isopropyl myristate (IPM) or isopropyl palmitate (IPP) uses a homogeneous or heterogeneous acid substance as a catalyst and is conducted at high temperatures and pressures. Utilization of this type of catalyst requires an additional postproduction process (neutralization and purification), which burdens the production cost. Microwave enzymatic esterification is a simple and fast method. The results showed that reaction time, ratio molar of fatty acids to isopropyl alcohol, catalyst weight, and microwave power have a significant effect on the IPM or IPP conversion. Further, the energy consumption of this process is less than other enzymatic esterification and is certainly more energy efficient, which could save 99 and 29% of processing time.

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Section: Resultsmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Microwave-Assisted Enzymatic Esterification Production of Emollient Esters

Sitepu,

Hudiyono,

Sinaga

et al. 2023

ACS Omega

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“…Enzymatic esterification reactions usually use low-volume methanol to avoid enzyme inhibition. 36 For reaction time, this study was performed in short reaction time compared to other studies. The homogenizer acid catalyst esterification method could save reaction time by 50–98%.…”

Section: Resultsmentioning

confidence: 99%

Room temperature esterification of high-free fatty acid feedstock into biodiesel

Supeno,

Sihotang,

Panjaitan

et al. 2023

RSC Adv.

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“…We can see the protective effect of Ca 2 Fe 2 O 5 on the enzyme by comparing the normalized maximum intensity in both systems as a function of temperature (Figure B). The contact with the support likely causes changes in the protein’s tertiary structure, increasing its rigidity and requiring a higher temperature to denature it. , …”

Section: Resultsmentioning

confidence: 99%

“…The contact with the support likely causes changes in the protein's tertiary structure, increasing its rigidity and requiring a higher temperature to denature it. 19,65 Analysis of the stability at pH revealed that immobilized lipase was significantly more stable than free lipase at an acidic pH, where the best residual activity values were found (Figure 6A). This may be associated with immobilization conditions.…”

Section: Stability Testsmentioning

confidence: 99%

Interfacial Hyperactivation of Candida rugosa Lipase onto Ca₂Fe₂O₅ Nanoparticles: pH and Ionic Strength Fine-Tuning to Modulate Protein-Support Interactions

Morales,

Hero,

Ledesma

et al. 2023

Langmuir

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The current study provides a comprehensive look of the adsorption process of Candida rugosa lipase (CRL) on Ca2Fe2O5 iron oxide nanoparticles (NPs). Protein-support interactions were identified across a broad range of pH and ionic strengths (mM) through a response surface methodology, surface charge determination, and spectroscopic and in silico analyses. The maximum quantity of immobilized protein was achieved at an ionic strength of 50 mM and pH 4. However, this condition did not allow for the greatest hydrolytic activity to be obtained. Indeed, it was recorded at acidic pH, but at 150 mM, where evaluation of the recovered activity revealed hyperactivation of the enzyme. These findings were supported by adsorption isotherms performed under different conditions. Based on zeta potential measurements, electrostatic interactions contributed differently to protein-support binding under the conditions tested, showing a strong correlation with experimentally determined immobilization parameters. Raman spectra revealed an increase in hydrophobicity around tryptophan residues, whereas the enzyme immobilization significantly reduced the phenylalanine signal in CRL. This suggests that this residue was involved in the interaction with Ca2Fe2O2 and molecular docking analysis confirmed these findings. Fluorescence spectroscopy showed distinct behaviors in the CRL emission patterns with the addition of Ca2Fe2O5 at pH 4 and 7. The calculated thermodynamic parameters indicated that the contact would be mediated by hydrophobic interactions at both pHs, as well as by ionic ones at pH 4. In this approach, this work adds to our understanding of the design of biocatalysts immobilized in iron oxide NPs.

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Fe2O3/Chitosan coated superparamagnetic nanoparticles supporting lipase enzyme from Candida Antarctica for microwave assisted biodiesel production

Cited by 33 publications

References 60 publications

Microwave-Assisted Enzymatic Esterification Production of Emollient Esters

Microwave-Assisted Enzymatic Esterification Production of Emollient Esters

Room temperature esterification of high-free fatty acid feedstock into biodiesel

Interfacial Hyperactivation of Candida rugosa Lipase onto Ca₂Fe₂O₅ Nanoparticles: pH and Ionic Strength Fine-Tuning to Modulate Protein-Support Interactions

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Fe2O3/Chitosan coated superparamagnetic nanoparticles supporting lipase enzyme from Candida Antarctica for microwave assisted biodiesel production

Cited by 33 publications

References 60 publications

Microwave-Assisted Enzymatic Esterification Production of Emollient Esters

Microwave-Assisted Enzymatic Esterification Production of Emollient Esters

Room temperature esterification of high-free fatty acid feedstock into biodiesel

Interfacial Hyperactivation of Candida rugosa Lipase onto Ca2Fe2O5 Nanoparticles: pH and Ionic Strength Fine-Tuning to Modulate Protein-Support Interactions

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Product

Resources

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Interfacial Hyperactivation of Candida rugosa Lipase onto Ca₂Fe₂O₅ Nanoparticles: pH and Ionic Strength Fine-Tuning to Modulate Protein-Support Interactions