Fe3O4 nanoparticles-enhanced SPR sensing for ultrasensitive sandwich bio-assay

Synthesis and characterization of Bodipy functionalized magnetic iron oxide nanoparticles for potential bioimaging applications

“…As a result compound, 6-(4-formylphenoxy)hexanoic acid (2.45 g) was obtained in 58% yield. 1 Bodipy-5 (5) was synthesized by reacting the compound 3 with 3-ethyl-2,4-dimethylpyrolle (4). In an oven dried 250 ml round bottom flask, dichloromethane (DCM) (200 ml) was added and bubbled under nitrogen gas for 20 min.…”

“…The final mixture was purified by column chromatography using CHCl 3 -MeOH (3%) eluent system. 1 FTIR spectra were recorded on a Bruker Tensor 37 FT-IR spectrometer in KBr while a Varian Cary 5000 was used for UV-vis spectrophotometric measurements. 1 H and 13 C NMR spectra were recorded on a Bruker Spectrospin Avance DPX400 Ultrashield spectrometer (400 and 100 MHz respectively) in CDCl 3 and internal standard TMS.…”

“…1 FTIR spectra were recorded on a Bruker Tensor 37 FT-IR spectrometer in KBr while a Varian Cary 5000 was used for UV-vis spectrophotometric measurements. 1 H and 13 C NMR spectra were recorded on a Bruker Spectrospin Avance DPX400 Ultrashield spectrometer (400 and 100 MHz respectively) in CDCl 3 and internal standard TMS. High resolution mass spectra were recorded with Agilent Technologies 6224 TOF LC/MS and 6530 Accurate Mass Q-TOF LC/MS, ionization method -ESI.…”

“…In the last decade, magnetic nanostructures have become a focus of many researches at chemistry-biology interface due to their biomedical applications such as magnetic bio-separation [1], magnetically targeted drug delivery [2], hyperthermia [3], magnetic resonance imaging [4], and magnetofection [5]. These kinds of nanostructures can be easily designed based on metallic, bimetallic, and iron oxide nanoparticles possessing magnetic property [6,7].…”

Nanotechnology-Based Surface Plasmon Resonance Affinity Biosensors forIn VitroDiagnostics

“…Thrombin was captured by immobilizing antithrombin aptamer on SPR gold film with a very low detection limit of 0.017 nM, thus confirming that MNPs are a powerful sandwich element and an excellent amplification reagent for SPR-based sandwich assays. The limit of detection obtained was lower than that obtained for the sandwich assay with AuNPS, but higher than with the use of rolling circle amplification [47]. …”