Feasibility of enriched mixed cultures obtained by repeated batch transfer in continuous hydrogen fermentation

Sivagurunathan, Periyasamy; Kumar, Gopalakrishnan; Park, Jeong Hoon; Park, Jong‐Hun; Park, Hee Deung; Yoon, Jeong Jun; Kim, Jun Seok

doi:10.1016/j.ijhydene.2015.06.133

Cited by 39 publications

(8 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Several researchers have also studied different microbial communities and their metabolic pathways based on a single type of substrate. For example, Sivagurunathan et al [ 33 ] reported that two Clostridium strains showed different metabolic pathways in different operation modes with galactose as substrate. However, little attention was paid to the relationship between the organic matter composition and the microbial mechanism, or details such as the microbial community and metabolic pathway.…”

Section: Resultsmentioning

confidence: 99%

Novel insight into the relationship between organic substrate composition and volatile fatty acids distribution in acidogenic co-fermentation

Zhang

et al. 2017

Biotechnol Biofuels

View full text Add to dashboard Cite

BackgroundCo-fermentation is an attractive technology for improving volatile fatty acids (VFAs) production by treatment of solid organic wastes. However, it remains unclear how the composition of different organic matters in solid waste influences the VFAs distribution, microbial community structure, and metabolic pathway during acidogenic co-fermentation. In this study, different organic wastes were added into waste activated sludge (WAS) as co-fermentation substrates to explore the impact of organic matter composition on VFAs pattern and the microbiological mechanism .ResultsAcetate was the most dominant VFA produced in all fermentation groups, making up 41.3–57.6% of the total VFAs produced during acidogenic co-fermentation under alkaline condition. With the increased addition of potato peel waste, the concentrations of propionate and valerate decreased dramatically, while ethanol and butyrate concentrations increased. The addition of food waste caused gradual decreases of valerate and propionate, but ethanol increased and butyrate was relatively stable. Some inconsistency was observed between hydrolysis efficiency and acidification efficiency. Our results revealed that starch was mainly responsible for butyrate and ethanol formation, while lipids and protein favored the synthesis of valerate and propionate. Microbial community analysis by high-throughput sequencing showed that Firmicutes had the highest relative abundance at phylum level in all fermentation groups. With 75% potato peel waste or 75% food waste addition to WAS, Bacilli (72.2%) and Clostridia (56.2%) were the dominant respective classes. In fermentation using only potato peel waste, the Bacilli content was 64.1%, while the Clostridia content was 53.6% in the food-only waste fermentation.ConclusionsAcetate was always the dominant product in acidogenic co-fermentation, regardless of the substrate composition. The addition of carbon-rich substrates significantly enhanced butyrate and ethanol accumulation, while protein-rich substrate substantially benefited propionate and valerate generation. Potato peel waste substantially favored the enrichment of Bacilli, while food waste dramatically increased Clostridia content in the sludge.Electronic supplementary materialThe online version of this article (doi:10.1186/s13068-017-0821-1) contains supplementary material, which is available to authorized users.

show abstract

Section: Resultsmentioning

confidence: 99%

Novel insight into the relationship between organic substrate composition and volatile fatty acids distribution in acidogenic co-fermentation

Zhang

et al. 2017

Biotechnol Biofuels

View full text Add to dashboard Cite

show abstract

“…Moreover, changes between continuous and discontinues modes have also been evaluated [30,31]. Final HRT for soluble substrates -such as monosaccharides-are 8-12 h [32], although some improvements have allowed to reduce it to 4 h not without metabolic unbalance risk [15,23].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of feeding strategies in upflow anaerobic sludge bed reactor for hydrogenogenesis at psychrophilic temperature

Rodríguez-Valderrama

Escamilla‐Alvarado

Amezquita-Garcia

et al. 2019

International Journal of Hydrogen Energy

View full text Add to dashboard Cite

The present work evaluated the biohydrogen production from a 0.4 L upflow anaerobic sludge blanket reactor type (UASB) operating at psychrophilic temperature (21 ±2 °C) at different feeding strategies varying hydraulic retention times (HRT) and sucrose concentration in the feeding. First strategy (24h/31c) fed semi-continuously 31 gsucrose L-1 at 24 h HRT; second strategy (12h/19c) fed semi-continuously 19 gsucrose L-1 at 12 h HRT; third strategy (4h/8c) fed continuously 8.3 gsucrose L-1 at 4 h HRT. After 70 days of operation, the UASB accumulated 65.44 L H2. The average HY for the whole operation during the three strategies was 62.6 NmL H2 gsucrose-1 , and average hydrogen content was 69.04%. In general terms, the best operation strategy was 12h/19c since it presented good set of results, the best HY (70.6 NmL H2 gsucrose-1) and a comparable hydrogen production rate (2.6 L (L d)-1) to that obtained in 4h/8c strategy (3.17 L (L d)-1). The average gross energy potential rate from the 12h/19c strategy was 46.21 kJ (L d)-1 , whereas energy heating losses were circumvented due to operation at psychrophilic regime. Indeed, psychrophilic or room temperatures should be broadly regarded as an effective alternative towards net energy gains in biohydrogen production.

show abstract

“…The amounts of hydrogen production for the assays through the modified Gompertz equation fitted with correlation coefficients R 2 over 0.9, which indicated the accuracy and precision of measurements [43], during the operation of the anaerobic reactors (Fig. 3).…”

Section: Kinetics Of Hydrogen Productionmentioning

confidence: 78%

Crude glycerol by transesterification process from used cooking oils: Characterization and potentialities on hydrogen bioproduction

Rodrigues

Santana

Nespeca

et al. 2016

International Journal of Hydrogen Energy

View full text Add to dashboard Cite

This study evaluated the potential bioconversion of crude glycerol from biodiesel production, applying used cooking oil for biohydrogen production by fermentative bacteria consortia. The pretreatment of crude glycerol was made by pH adjustment. Heat treatment of the inocula and initial pH 5.5 were applied to select hydrogen-producing bacteria and inactivate hydrogen-consumers microorganisms. The inocula tested were: (I) granular sludge from the thermophilic UASB reactor used on the treatment of vinasse and (II) granular sludge from the UASB reactor used on the treatment of sanitary sludge for the assays (1) and (2), respectively. The characterization of crude glycerol presented high levels of alkalinity, methanol and soap that may be inhibitory to biologic processes of H 2 production. The assays were carried in anaerobic batch reactors in order to verify the efficiencies of crude glycerol to H 2 generations by the microbial consortia (20%) at 37 C, initial pH 5.5, with 20.0 g COD L À1 glycerol. The cumulative production of hydrogen for the assays (1) and (2) were, respectively, (mmol H 2 L À1) 28.49 ± 1.55 and 19.14 ± 1.67. The subsequent yields were obtained as follows: 2.2 moL H 2 mol À1 glycerol and 1.1 moL H 2 mol À1 glycerol, respectively. The used cooking oil was an efficient waste for bioconversion of crude glycerol to H 2 production.

show abstract

Feasibility of enriched mixed cultures obtained by repeated batch transfer in continuous hydrogen fermentation

Cited by 39 publications

References 49 publications

Novel insight into the relationship between organic substrate composition and volatile fatty acids distribution in acidogenic co-fermentation

Novel insight into the relationship between organic substrate composition and volatile fatty acids distribution in acidogenic co-fermentation

Evaluation of feeding strategies in upflow anaerobic sludge bed reactor for hydrogenogenesis at psychrophilic temperature

Crude glycerol by transesterification process from used cooking oils: Characterization and potentialities on hydrogen bioproduction

Contact Info

Product

Resources

About